Research experience
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Aug 2011–
Jul 2012Research: phenotypic and genotypic characterisation of vancomycin resistance enterococci
NIPER · genetic laboratory GMCH · NIPERGUWAHATIANTIBIOTIC RESISTANCE
Education
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Jun 2010–
Jun 2012National Institute of Pharmaceutical Education and Research
M.S.(PHARM) BIOTECHNOLOGYIndia · Guwahati
Other
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LanguagesEnglish, Hindi, Marathi, Gujrati
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Other Interests"Wings of fire " by Abdul kalam.
Questions and Answers (31) View all
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Answer added in Antibiotics12 Plasmid isolation of gram positive bacteria by heat lysis using lysosymeBy Swapnil Ranotkar · National Institute of Pharmaceutical Education and ResearchSwapnil Ranotkar · National Institute of Pharmaceutical Education and ResearchI am using the lysosyme at the conc. Of 5mg/ml and incubated it for 1 hr heat at 100 c for 15 minute.I am using the lysosyme at the conc. Of 5mg/ml and incubated it for 1 hr heat at 100 c for 15 minute.Following
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Answer added in Antibiotics12 Plasmid isolation of gram positive bacteria by heat lysis using lysosymeBy Swapnil Ranotkar · National Institute of Pharmaceutical Education and ResearchSwapnil Ranotkar · National Institute of Pharmaceutical Education and ResearchThanks for all the kind reply .Thanks for all the kind reply .Following
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Question asked in Antibiotics12 Plasmid isolation of gram positive bacteria by heat lysis using lysosymeI am working on antimicrobial resistance . I tried the isolation of the bacteria plasmid using a lysis method but i'm unable to recover the plasmid by... [more]I am working on antimicrobial resistance . I tried the isolation of the bacteria plasmid using a lysis method but i'm unable to recover the plasmid by heat lysis. I use the lysosyme for the breakage of the cell wall but it doesn't work. What do you think is the most likely reason; should I first use another lysosome, change the concentration or use another detergent on the side?By Swapnil Ranotkar · National Institute of Pharmaceutical Education and ResearchFollowing
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Answer added in DNA Isolation17 Plasmid isolation.By Swapnil Ranotkar · National Institute of Pharmaceutical Education and ResearchSwapnil Ranotkar · National Institute of Pharmaceutical Education and Researchdoes boiling method work for the gram positive bacteria because i'm working on Enterococcidoes boiling method work for the gram positive bacteria because i'm working on EnterococciFollowing