Suraj Priyanka

Publications

• Neuroprotective role of Melatonin against aluminum-induced oxidative stress in the hippocampus of mouse brain

  N. John Sushma, S. Priyanka, K. Jayantha Rao

  Journal of Applied Pharmaceutical Science. 12/2011; 1(10):126-133.

  We evaluated the effect of melatonin (Mel) in male albino mice which received aluminum acetate (Al) for 6 weeks (3.5 mg/kg body weight) (b.w.) i.p. five times per week. Moreover mice received Mel (7mg/kg b.w.i.p. 5 days/week) for 6 weeks. At the end of the treatment hippocampus was removed and proce... [more] We evaluated the effect of melatonin (Mel) in male albino mice which received aluminum acetate (Al) for 6 weeks (3.5 mg/kg body weight) (b.w.) i.p. five times per week. Moreover mice received Mel (7mg/kg b.w.i.p. 5 days/week) for 6 weeks. At the end of the treatment hippocampus was removed and processed to examine the oxidative stress markers. Following Al exposure oxidative stress increased significantly, estimated by increased thiobarbituric acid reactive substances (TBARS) and decrease in the activity of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), reduced glutathione (GSH),glutathione peroxidase (GPx) and glutathione-s-transferase (GST). Al+Mel treatment significantly prevented the aluminum induced decrease in antioxidant enzymes as well as decrease in TBARS. Histopathological evidence in the hippocampi revealed the protective effect of melatonin against Al induced damage in light and transmission electron microscopic (TEM) studies. These results supported that melatonin suppresses the oxidative stress. This may result from the higher efficacy of melatonin in scavenging various free radicals and also because of its ability in stimulating the anti oxidant enzymes.
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  Impact points

  Biosynthesis, characterization and antimicrobial studies of AgNPs extract from bacopa monniera whole plant

  B. Mahitha, B. Deva, Prasad Raju, G. R. Dillip, C. Madhukar Reddy, L. Manoj, S.Priyanka, K.Jayantha Rao, N. John Sushma

  Digest Journal of Nanomaterials and Biostructures. 01/2011; 6:135-142.

  Reliable and eco-friendly process for synthesis of metallic nanoparticles is an important step in the field of the nanotechnology. One of the options to achieve this objective is to use biological systems. Here we report the ethanolic extract of B. monniera to produce silver nanoparticles by reducti... [more] Reliable and eco-friendly process for synthesis of metallic nanoparticles is an important step in the field of the nanotechnology. One of the options to achieve this objective is to use biological systems. Here we report the ethanolic extract of B. monniera to produce silver nanoparticles by reduction of AgNO3. It was observed that the synthesis process was quite rapid and silver nanoparticles were formed with in minutes of silver ion coming in contact with the plant filtrate. UV-visible spectrum of the aqueous medium containing silver ions demonstrated a peak at 436 nm corresponding to the plasmon absorbance of silver nanoparticles. X-Ray diffraction (XRD) analysis reveals the FCC structure of silver nanoparticles. Energy dispersive X-ray analysis (EDX) evidences the presence of silver nanoparticles in the aquatic solution of leaf extract. FTIR analysis of the nanoparticles indicated the presence of proteins, which may be acting as capping agents around the nanoparticles. From transmission electron microscopy (TEM) analysis, the size of the silver nanoparticles was measured (10-30 nm). Further the antimicrobial activity of synthesized particles showed effective inhibitory activity.

• . Melatonin prevents aluminum-induced oxidative damage and cytotoxicity in the medulla oblongata of mouse brain

  S. Priyanka, B. Mahitha, K. Jayantha Rao, N. John Sushma

  Journal of Pharmacy Research. 01/2011; 4:683-686.

  We examined the daily melatonin (Mel) supplementation on oxidative damage and cytotoxicity induced by Aluminum (Al) in medulla oblongata of mice. Al induced pro-oxidant activity and the protective role of melatonin was determined in the medulla oblongata of mice following administration of Al and/or... [more] We examined the daily melatonin (Mel) supplementation on oxidative damage and cytotoxicity induced by Aluminum (Al) in medulla oblongata of mice. Al induced pro-oxidant activity and the protective role of melatonin was determined in the medulla oblongata of mice following administration of Al and/or melatonin. Two groups of male mice were intraperitoneally injected with Al (as Al acetate) or melatonin only, at doses of 3.5 and 7 mg/kg/day, respectively, for 6 weeks. During this period, a third group of animals received Al (3.5 mg/kg/day) plus melatonin (7mg/ kg/day). At the end of the treatment medulla oblongata were isolated and the oxidative stress markers: superoxide dismutase (SOD), catalase (CAT) and thiobarbituric acid-reactive substances (TBA-RS) were carried out. Protective effects of Mel against Al were also observed at microscopic level. Light microscopy of Al administered medulla oblongata showed severe hemorrhagic areas, congested blood vessels, karyopycnosis of nucleus and vacuolated cells which are antagonized by Mel supplementation. Ultra structural studies revealed degenerated nerve fibres and complete cytoarchitectural loss of neurons in medulla oblongata of Al treated which showed improvement following Mel. The results show that the biochemical and ultra structural changes observed in this tissue indicate that Al acts as pro-oxidant agent, while melatonin exerts antioxidant action.