Suman Kapila

Publications (36) View all

• Article: Effect of thermal processing of cow and buffalo milk on the allergenic response to caseins and whey proteins in mice.

  Umesh Kumar Shandilya, Rajeev Kapila, Raies M Haq, Suman Kapila, Vk Kansal
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  ABSTRACT: BACKGROUND: Heat treatment is the most common method for reducing pathogen load, but it remains controversial in reducing the incidence of hyperimmune reactions. The aim of this study was to compare the allergenicity of caseins (CSN) and whey proteins (WP) of thermally processed cow and buffalo milk in a mouse model. Swiss albino mice were sensitised by intraperitoneal injections (administered in three doses at weekly intervals) of CSN or WP from cow or buffalo milk for the evaluation of humoral response and splenocyte stimulation index. RESULTS: After 3 weeks of intraperitoneal stimulation of mice with milk proteins, the sterilised milk protein group displayed significantly lowered (P ≤ 0.05) serum IgG and IgE levels, while considerably increased cow milk protein-specific responses (IgE) were shown by proteins of pasteurised milk compared with those of raw milk. The stimulation index of splenocytes induced by CSN or WP of boiled and sterilised milk was also lower (P ≤ 0.05) than that of raw milk of both cow and buffalo. CONCLUSION: The experiment showed that boiling and sterilisation of cow and buffalo milk clearly affect the allergenicity by decreasing the humoral and cell-mediated responses in mice. All results indicated that CSN and WP of sterilised milk are less allergenic than those of raw milk in mice. © 2013 Society of Chemical Industry.
  Journal of the Science of Food and Agriculture 01/2013; · 1.44 Impact Factor
• Article: Effect of micronutrient supplementation around calving on the plasma cortisol levels of Murrah buffaloes and Sahiwal and Karan Fries cows.

  Ajay Kumar Dang, Manu Jamwal, Mandheer Kaur, Shiv Prasad Kimothi, Shashi Pal, Kalyan De, Mohsin M Pathan, Dilip K Swain, Sushil K Mohapatra, Suman Kapila, Rajeev Kapila, Harjit Kaur, Ashok K Mohanty, Bukkaraya S Prakash
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  ABSTRACT: Micronutrients when fed around peripartum may reduce the stress induced by cortisol. With this objective, 24 Sahiwal (SW) and 24 Karan Fries (KF) cows and 24 Murrah (Mu) buffaloes were taken and divided into four groups of six each. Vitamin E (VE), zinc (Zn) and copper were supplemented from 30 days pre- to 30 days postcalving in groups 1, 2 and 3. Animals without supplementation served as control. Blood sampling was done on days 30, 15, 7 and 3 precalving; at calving; and on days 3, 7, 15 and 30 postcalving. Plasma cortisol levels were measured by ELISA. Supplementation of VE and Zn significantly (P < 0.05) reduced plasma cortisol levels at calving and at 30 days postcalving. KF cows exhibited best results with VE, whereas SW cows and Mu buffaloes responded best to Zn. Copper increased the peripartum cortisol levels. Reduction in cortisol levels may help in keeping the animal healthy and stronger to fight immuno-suppression generally observed around the period of peripartum.
  Tropical Animal Health and Production 11/2012; · 1.12 Impact Factor
• Article: Resistance of Microencapsulated Lactobacillus acidophilus LA1 to Processing Treatments and Simulated Gut Conditions

  Latha Sabikhi, R. Babu, D. K. Thompkinson, Suman Kapila
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  ABSTRACT: This investigation reports the effect of microencapsulation using sodium alginate and starch on the tolerance of probiotic Lactobacillus acidophilus LA1 to selected processing conditions and simulated gastrointestinal environments. The organism survived better in the protected form at high temperatures (72, 85, and 90°C) and at high salt concentrations (1%, 1.5%, and 2%). The free cells were completely destroyed at 90°C whereas the microencapsulated cells reduced by 4.14 log cycles. The log cycle reduction was 5.67 and 2.30, respectively, in free and protected cells when incubated for 3h with 2% (w/v) NaCl. Homogenization did not affect the viability of the cells but led to the disruption of the protective encapsulating material around the cells. Microencapsulation provided better protection at simulated conditions of gastric pH (1.0, 1.5, and 2.0) and at high bile salt concentrations (1.0%, 1.5%, and 2.0%). The free and protected cells registered 5.47 and 2.16 log cycle reduction, respectively, after 3-h incubation at 2% bile salt (w/v). The release of the microencapsulated organisms in simulated colonic pH required 2.5h. These studies demonstrated that microencapsulation of probiotic L. acidophilus LA1 in sodium alginate is an effective technique of protection against extreme processing conditions and under simulated gastrointestinal environment. KeywordsMicroencapsulation-Probiotic-Lactobacilli-Survival-Processing
  Food and Bioprocess Technology 04/2012; 3(4):586-593. · 3.70 Impact Factor
• Conference Proceeding: Activity of blood leukocytes around parturition in high producing crossbred cows

  Mandira Chaudhury, Joydip Mukherjee, Suman Kapila, A.K. Mohanty, A.K. Dang
  International Conference On Frontiers in Reproductive Biotechnology, NDRI, Karnal; 01/2011
• Article: Anti-allergic effects of probiotic Dahi through modulation of the gut immune system.

  Shalini Jain, Hariom Yadav, P R Sinha, Suman Kapila, Yasuhiro Naito, Francesco Marotta
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  ABSTRACT: The alarming increase in allergy in the last few decades demands the development of new anti-allergic prevention strategies, and consumption of functional foods (i.e. probiotic Dahi, which has already been proven to enhance immunity by modulation of the gut mucosal immune system) may be one of them. In the present study, we evaluated anti-allergic effects of a Dahi (yogurt) containing probiotic Lactobacillus acidophilus, L. casei and normal Dahi culture Lactococcus lactis biovar diacetylactis (named probiotic Dahi) on ovalbumin induced allergy in mice. Allergy was induced by injecting (i.p.) ovalbumin at 0 and 14 days. Animals were fed with standard diet (control), milk, control Dahi or probiotic Dahi for 21 days. Total and ovalbumin-specific IgE, cytokines and lymphocyte proliferation index were examined after 7, 14 and 21 days. Feeding of probiotic Dahi completely suppressed the elevation of total and ovalbumin-specific IgE in the serum of ovalbumin-injected mice. Similarly, splenocytes collected from mice fed with probiotic Dahi entirely lost the total and ovalbumin-specific IgE production property during in-vitro culture. Production of T helper (Th)-1 cell-specific cytokines, i.e. interferon -γ and interleukin (IL)-2, increased, while Th2-specific cytokines, i.e. IL-4 and IL-6, decreased in the supernatant of cultured splenocytes collected from mice fed with probiotic Dahi compared to the other groups. Moreover, ovalbumin-stimulated lymphocyte proliferation was strongly suppressed by feeding of probiotic Dahi in comparison to milk and control Dahi. Results of the present study indicate that probiotic Dahi suppressed ovalbumin-induced allergic consequences characterized by decreasing levels of total and ovalbumin-specific IgE and lymphocyte proliferation and skewed ovalbumin-induced Th2-specific immune response towards Th1-specific response.
  The Turkish journal of gastroenterology: the official journal of Turkish Society of Gastroenterology 09/2010; 21(3):244-50. · 0.47 Impact Factor