Publications (6) View all
-
Article: Molecular markers and doubled haploids in European plant breeding programmes
[show abstract] [hide abstract]
ABSTRACT: The breeding companies and laboratories involved in this article cover a wide range of crops grown in the temperate climate zone: small grain cereals, oilseed crops, forage crops, turf, vegetables and potato. Speed and efficiency are becoming increasingly important in variety breeding and doubled haploids (DH) and genetic markers are important biotechnological tools to accelerate materials to market. Collaborative research between universities, research institutions and breeding companies has resulted in the routine use of DH technology and molecular markers in practical breeding of barley, wheat and rapeseed. DH populations have been established not only for barley, wheat and rapeseed, but for rye, oat and triticale, where DH technology is less developed. A driver here is the value of the crop e.g. although wheat is less responsive to DH production the value of the end product makes the effort worthwhile. Simple and rapid DNA extraction methods used in high-throughput marker assisted selection (MAS) systems are essential for routine use of markers. MAS is used both to monitor the presence of genes of interest and also to monitor the genetic background. DH technology in forage, turf and vegetables is still in progress and the practical use of markers in all crops is limited by access to trait linked markers. Collaboration and technology transfer with universities, research institutions and breeding companies is essential for the improvement of both DH protocols in recalcitrant crops and marker technology in all crops.Euphytica 04/2012; 158(3):305-312. · 1.55 Impact Factor -
Article: A high-throughput DNA extraction method for barley seed
[show abstract] [hide abstract]
ABSTRACT: A non-destructive, quick DNA extraction method for barley seed is described. The method is simple and consists of drilling out a sample from the seed, adding sodium hydroxide, heating in a microwave oven and neutralizing with Tris-HCl. The seed DNA extract can be used directly for PCR with extra cycles added to the PCR programme compared to PCR programmes used for leaf extracts. This protocol was developed in particular for a micro satellite marker genetically linked to barley yellow mosaic virus resistance, but it can be applied toother markers of interest for barley breeding. The quick seed extraction protocol makes it possible to handle thousands of samples per day. Extraction of DNA from seed also facilitates transfer of plant material compared to the long-distance transfer of leaf samples.Euphytica 04/2012; 130(2):255-260. · 1.55 Impact Factor -
Article: New DArT markers for oat provide enhanced map coverage and global germplasm characterization.
[show abstract] [hide abstract]
ABSTRACT: Background: Genomic discovery in oat and its application to oat improvement have been hindered by a lack of genetic markers common to different genetic maps, and by the difficulty of conducting whole-genome analysis using high-throughput markers. This study was intended to develop, characterize, and apply a large set of oat genetic markers based on Diversity Array Technology (DArT). Results: Approximately 19,000 genomic clones were isolated from complexity-reduced genomic representations of pooled DNA samples from 60 oat varieties of global origin. These were screened on three discovery arrays, with more than 2000 polymorphic markers being identified for use in this study, and approximately 2700 potentially polymorphic markers being identified for use in future studies. DNA sequence was obtained for 2573 clones and assembled into a non-redundant set of 1770 contigs and singletons. Of these, 705 showed highly significant (Expectation < 10E-10) BLAST similarity to gene sequences in public databases. Based on marker scores in 80 recombinant inbred lines, 1010 new DArT markers were used to saturate and improve the 'Kanota' x 'Ogle' genetic map. DArT markers provided map coverage approximately equivalent to existing markers. After binning markers from similar clones, as well as those with 99% scoring similarity, a set of 1295 non-redundant markers was used to analyze genetic diversity in 182 accessions of cultivated oat of worldwide origin. Results of this analysis confirmed that major clusters of oat diversity are related to spring vs. winter type, and to the presence of major breeding programs within geographical regions. Secondary clusters revealed groups that were often related to known pedigree structure. Conclusion: These markers will provide a solid basis for future efforts in genomic discovery, comparative mapping, and the generation of an oat consensus map. They will also provide new opportunities for directed breeding of superior oat varieties, and guidance in the maintenance of oat genetic diversity.BMC Genomics 01/2009; · 4.07 Impact Factor -
Chapter: Automation of DNA Marker Analysis for Molecular Breeding in Crops
[show abstract] [hide abstract]
ABSTRACT: Plant breeders constantly need to adapt their research to the ever-changing market needs and agricultural practices. To achieve these goals, they need to competently combine different genetically-governed characters in a genotype, this is a complex, time-consuming and labour intensive task. In modern plant breeding, molecular markers are of increasing importance, and it is today undeniable that their application inhold tremendous possibilities to increase plant breeding efficiency. While the methods are more widely adopted, the capacity for high-throughput analyses at low cost becomes crucial for their practical use. To be attractive it is necessary that molecular technology is able to promptly handle sufficiently large amounts of material at reduced costs. Automation of the analysis processes is a way to meet these requirements. In that purpose, the specific needs of molecular applications in practical plant breeding are investigated in this chapter. The particular approach of a plant breeding company to automate them, in order to increase their availability to breeding programs, is described.12/2008: pages 143-161; -
Article: Foreword: Gametic cells and molecular breeding for crop improvement
Euphytica 01/2007; 158(3):273-274. · 1.55 Impact Factor
