Publications (54) View all
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Article: Injection of human mesenchymal stem cells improves healing of scarred vocal folds: Analysis using a xenograft model
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ABSTRACT: Objectives/Hypothesis:The aims were to analyze if improved histological and viscoelastic properties seen after injection of human mesenchymal stem cells (hMSCs) in scarred vocal folds (VFs) of rabbits are sustainable and if the injected hMSCs survive 3 months in the VFs.Study Design:Experimental xenograft model.Methods:Eighteen VFs of 11 New Zealand white rabbits were scarred by a bilateral localized resection. After 3 months the animals were sacrificed. Twelve VFs were dissected and stained for histology, lamina propria thickness, and relative collagen type I analyses. The hMSCs survival was analyzed using a human DNA-specific reference probe, that is, fluorescence in situ hybridization staining. Viscoelasticity, measured as the dynamic viscosity and elastic modulus, was analyzed in a parallel-plate rheometer for 10 VFs.Results:The dynamic viscosity and elastic modulus of hMSC-treated VFs were similar to that of normal controls and significantly improved compared to untreated controls (P < .05). A reduction in lamina propria thickness and relative collagen type 1 content were also shown for the hMSC-treated VFs compared to the untreated VFs (P < .05). The histological pictures corresponded well to the viscoelastic results. No hMSCs survived.Conclusions:Human mesenchymal stem cells injected into a scarred vocal fold of rabbit enhance healing of the vocal fold with reduced lamina propria thickness and collagen type I content and restore the viscoelastic function. Laryngoscope, 2010The Laryngoscope 06/2010; 120(7):1370 - 1375. · 1.75 Impact Factor -
Article: Effects of botulinum toxin treatment for adductor spasmodic dysphonia on voice and social function
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ABSTRACT: Thirty-nine patients with adductor spasmodic dysphonia currently under treatment with botulinum toxin estimated their vocal and social function before and during treatment in percentage of normal. Subjective symptoms and side effects were registered. All patients had positive effects. Mean improvement was 45 per cent for voice function and 47 per cent for social function. Eleven patients (28 per cent) claimed they had a completely normal voice for a short period between injections. Thirty-three patients (85 per cent) reported a considerably breathy voice quality lasting several weeks after injection on one or more occasions. Sixteen patients had experienced slight deglutition problems. In spite of these side effects, the results confirm that the overall efficacy of treatment of adductor spasmodic dysphonia with botulinum toxin is very high.07/2009; 22(3):129-134. -
Article: A comparison between manual and automatic flow inverse filtering for patients with spindle-shaped glottis during phonation
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ABSTRACT: Twenty-eight patients showing spindle-shaped glottal insufficiency with or without sulcus vocalis at laryngoscopic examination were examined with videostroboscopy, flow inverse filtering and audio recordings of voice. Inverse filtering was performed both manually and with an automatic method. No major differences were found between the inverse filtering methods regarding glottal flow or estimations of glottal closure and voice quality from flow data. Several flow parameters correlated well with perceived breathiness in the voice. The sulcus patients had significantly higher flow open quotient (OQ 20%), higher mean flow and higher fundamental frequency during phonation than the non-sulcus patients. Male sulcus patients also had higher estimated minimum area values than the male non-sulcus patients. The presence (or absence) of a hump in the closed phase of the glottograms was related to the presence (or absence) of a mucosal wave for the patients studied.07/2009; 19(3):117-134. -
Article: Vocal fold length measurements with computed tomography
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ABSTRACT: Computed tomography (CT) was used for vocal fold length measurements in a pilot study on five normal subjects. Repeated phonations at a stable pitch showed a maximal length variation of 3% between measurements. An increase in voice intensity for fixed phonation pitch resulted in a minor shortening of the vocal fold length. This shortening was, however, within the measurement error. The length of the membranous part of the vocal folds determined from CT examination appears to be a suitable distance for calibrations if absolute glottal area or lengths are to be measured from video recorded laryngeal examinations with flexible or rigid endoscopes. The method requires that the subject or patient is able to produce repeated phonations matching a given pitch.07/2009; 18(2-3):57-63. -
Article: Elasticity measurements in scarred rabbit vocal folds using air pulse stimulation.
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ABSTRACT: This study aimed to compare vocal fold elasticity data from a new method for non-invasive analysis by stimulations of the mucosa with short air pulses. The depth of the mucosal deflections is measured with laser pulses by means of a special algorithm. Ten scarred New Zealand rabbit vocal folds and four normal rabbit folds were measured directly after sacrifice. The elastic data were compared to histological sections from the scarred vocal folds analysed by a pathologist. The results showed significantly lower elasticity (higher stiffness) values for the more scarred vocal folds as compared to samples with minor damage (P=0.03). It is concluded that the air pulse stimulation method is a promising tool for non-invasive quantification of vocal fold scarring.Logopedics, phoniatrics, vocology 06/2009; 34(4):190-5.
