Article: Recent advances in high resolution scanning electrochemical microscopy of living cells - A review.[show abstract] [hide abstract]
ABSTRACT: This review discusses advances in the field of high resolution scanning electrochemical microscopy (HR-SECM) and scanning ion conductance microscopy (SICM) to study living cells. Relevant references from the advent of this technique in the late 1980s to most recent contributions in 2012 are presented with special discussion on high resolution images. A clear progress especially within the last 5 years can be seen in the field of HR-SECM. Furthermore, we also concentrate on the intrinsic properties of SECM imaging techniques e.g. different modes of image acquisition, their advantages and disadvantages in imaging living cells and strategies for further enhancement of image resolution, etc. Some of the recent advances of SECM in nanoimaging have also been discussed which may have potential applications in high resolution imaging of cellular processes.Analytica chimica acta 05/2013; 775:1-13. · 4.31 Impact Factor
Article: Monitoring passive transport of redox mediators across a confluent cell monolayer with single-cell resolution by means of scanning electrochemical microscopyAnalytical Methods. 01/2012; 4:623-629.
Article: High-resolution imaging of nanostructured Si / SiO2 substrates and cell monolayers using scanning electrochemical microscopyElectroanalysis 01/2011; 23(1):196-200. · 2.87 Impact Factor
Article: Simultaneous imaging and chemical attack of a single living cell within a confluent cell monolayer by means of scanning electrochemical microscopy.[show abstract] [hide abstract]
ABSTRACT: Epithelial cell monolayers from rat kidney were imaged by scanning electrochemical microscopy (SECM) with sub-micrometer resolution in both lateral and vertical direction. Platinum disk ultra-microelectrodes (UMEs) with effective electrode radii between 200 and 600 nm were operated in the constant-height mode. The quality of the recorded SECM images compare favorably with those of phase contrast and confocal laser scanning microscopy. Besides the acquisition of SECM images, the UME was used to selectively attack a single living cell within the monolayer ensemble. Hydroxide ions were locally generated in the vicinity of a single target cell by the UME. The increase in pH induced cell necrosis that was subsequently imaged by SECM. It could be clearly demonstrated that the single target cell was selectively affected, whereas the adjacent reference cells remained unchanged.Analytical Chemistry 01/2011; 83(1):169-74. · 5.86 Impact Factor