Srikanth Aluru

Publications

• Expression,Purification and Quantification of Neuraminidase gene of Influenza

  Dr.Archana Giri, Srikanth Aluru

  Dissertation work. 01/2010;

  Avian Influenza outbreaks among poultries are occur world wide from time to time with increasingGlobal pandemics. The strategies to prevent and test for avian influenza are becoming increasingly more important. One strain of avian influenza, H5N1, has already crossed the species border into humans. ... [more] Avian Influenza outbreaks among poultries are occur world wide from time to time with increasingGlobal pandemics. The strategies to prevent and test for avian influenza are becoming increasingly more important. One strain of avian influenza, H5N1, has already crossed the species border into humans. We propose a method for developing a subunit vaccine and influenza tests usingBacteria as the chassis, respectively. This system is easily amendable for targeting the two mainAntigens presented on the exterior of the influenza virus: Hemagglutinin and Neuraminidase. Thepurification and quantificationof Neuraminidase protein in this study will provide useful information further avian influenza vaccine & Diagnostics development. Recombinant envelop protein of H5N1, 55KDa of rNA1, was successfully produced in prokaryotic system by using pRSET/A expression vector, and the expression was optimized by IPTG, this shows the ideal condition to obtain high yield of rNA1 protein. The result of purification showed that the expressed protein purified form of rNA1.The quantification analysis through calorimetric assay revealed yield of recombinant protein rNA1 protein respectively. This experimental procedurecompletely shows production of recombinant AIV/NA protein in bacteriachassis especially in E coli for high yields at low cost & lesser time effectively.

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• A poster of my Dissertation Work

  Dr. Archana Giri, Srikanth Aluru

  Jawaharlal nehru technological university -Research Bank. 01/2010;