sri karthika Shanmugam

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• Answer added in Transfection
  12 I'm looking for some tips on improving co-transfection of 293 T cells with 3 plasmids (one GFP).
  By sri karthika Shanmugam · Stowers Institute for Medical Research
  sri karthika Shanmugam · Stowers Institute for Medical Research

  Thank you very much all! 

  Thank you very much all!
  Following
• Question asked in Transfection
  12 I'm looking for some tips on improving co-transfection of 293 T cells with 3 plasmids (one GFP).
  I have been using the Calcium phosphate method, but I couldn't get any expression. Could purity of DNA be a problem? Can I use lipofactamine 2000 for... [more]
  I have been using the Calcium phosphate method, but I couldn't get any expression. Could purity of DNA be a problem? Can I use lipofactamine 2000 for better results?
  By sri karthika Shanmugam · Stowers Institute for Medical Research
  Following