Sreenivasan Sasidharan

Publications (90) View all

• Source

  Available from: Vijayarathna Soundararajan

  Article: Evaluation of the effect of Cassia surattensis Burm. f., flower methanolic extract on the growth and morphology of Aspergillus niger

  V. Sumathy, Z. Zakaria, Y. Chen, L.Y. Latha, S.L. Jothy, S. Vijayarathna, S. Sasidharan
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  ABSTRACT: Cassia (C.) surattensis Burm. f. (Leguminosae), a medicinal herb native to tropical equatorial Asia, was commonly used in folk medicine to treat various diseases.The aim of the present study is to investigate the effects of methanolic flower extract of C. surattensis against Aspergillus (A.) niger.Antifungal activity of C. surattensis flower extract was studied by using agar disc diffusion method, broth dilution method, percentage of hyphal growth inhibition and scanning electron microscopy (SEM) observation.The extract exhibited good antifungal activity with zone of inhibition 15 mm and minimum inhibitory concentration (MIC) 6.25 mg/ml. The flower extract exhibited considerable antifungal activity against A. niger with a IC50 of 2.49 mg/ml on the hyphal growth. In scanning electron microscopy (SEM) squashed, collapsed, empty and deformation of hyphae were the major changes observed. Shrunken conidiophores were the obvious alteration on the spores. Morphological alterations observed on A. niger caused by the flower extract could be the contribution of chemical compounds present in the Cassia flower. Phytochemical screening reveals the presence of carbohydrate, tannins, saponins and phenols in the extract. The amount of tannin, total phenolics and flavonoids were estimated to be 55.14 ± 3.11 mg/g, 349.87 ± 5.41 mg/g gallic acid equivalent and 89.64 ± 3.21 mg/g catechin equivalent respectively. C. surattensis flower extract potently inhibited the growth of A. niger and are, therefore, excellent candidates for use as the lead compounds for the development of novel antifungal agents.
  European review for medical and pharmacological sciences 01/2013; · 1.04 Impact Factor
• Source

  Available from: Sreenivasan Sasidharan

  Chapter: Screening Methods in the Study of Fungicidal Property of Medicinal Plants

  S. Sasidharan, L. Yoga Latha, Kwan Yuet Ping, S. Jothy Lachumy
  01/2012; , ISBN: 978-953-307-804-5
• Article: Anti-Candida activity and brine shrimp toxicity assay of Ganoderma boninense.

  K M A Daruliza, L Fernandez, R Jegathambigai, S Sasidharan
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  ABSTRACT: Ganoderma (G.) boninense is a white rot fungus, which can be found in the palm oil tree. Several studies have shown that G. boninense has antimicrobial and antagonistic properties. However, there is limited information reported on antifungal properties especially on Candida (C) albicans. Hence, this study was conducted to determine the anti-Candida activity of G. boninense against C albicans. Crude methanolic extracts of G. boninense was obtained by maceration method with 70% methanol. Anti-Candida test was carried out using disc diffusion assay, broth dilution method, time killing profile and brine shrimp toxicity assay. Anti-Candida activity indicated that the mean zone of inhibition was 12.5 +/- 0.6 mm. The MIC value for C. albicans found to be 3.125 mg/ml. The result from time-killing profile showed that the growth of C albicans was inhibited hence decreases its exponential phase. For brine shrimp toxicity assay, the LC50 value was 3.59 mg/ml which proved that the extract of G. boninense is not toxic.
  European review for medical and pharmacological sciences 01/2012; 16(1):43-8. · 1.04 Impact Factor
• Article: Optimal protein extraction methods from diverse sample types for protein profiling by using Two-Dimensional Electrophoresis (2DE).

  A A Tan, S N Azman, N R Abdul Rani, B C Kua, S Sasidharan, L V Kiew, N Othman, R Noordin, Y Chen
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  ABSTRACT: There is a great diversity of protein samples types and origins, therefore the optimal procedure for each sample type must be determined empirically. In order to obtain a reproducible and complete sample presentation which view as many proteins as possible on the desired 2DE gel, it is critical to perform additional sample preparation steps to improve the quality of the final results, yet without selectively losing the proteins. To address this, we developed a general method that is suitable for diverse sample types based on phenolchloroform extraction method (represented by TRI reagent). This method was found to yield good results when used to analyze human breast cancer cell line (MCF-7), Vibrio cholerae, Cryptocaryon irritans cyst and liver abscess fat tissue. These types represent cell line, bacteria, parasite cyst and pus respectively. For each type of samples, several attempts were made to methodically compare protein isolation methods using TRI-reagent Kit, EasyBlue Kit, PRO-PREP™ Protein Extraction Solution and lysis buffer. The most useful protocol allows the extraction and separation of a wide diversity of protein samples that is reproducible among repeated experiments. Our results demonstrated that the modified TRI-reagent Kit had the highest protein yield as well as the greatest number of total proteins spots count for all type of samples. Distinctive differences in spot patterns were also observed in the 2DE gel of different extraction methods used for each type of sample.
  Tropical biomedicine 12/2011; 28(3):620-9. · 0.62 Impact Factor
• Article: In vitro and in situ antiyeast activity of Gracilaria changii methanol extract against Candida albicans.

  S Sasidharan, I Darah, K Jain
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  ABSTRACT: The Gracilaria (G.) sp are widely used in the traditional medicine in Malaysia. The methanol extract of Gracilaria changii B.M. Xia & I.A. Abbott (Gracilariaciae) was evaluated for antiyeast activity against Candida albicans (Berkhout). The antiyeast activities were studied by using disc diffusion method and broth dilution method. The effect of the extract on the growth profile of the yeast was also examined via time-kill assay. In addition, the in situ antiyeast activity was studied by microscopic observations using scanning electron microscopy (SEM), transmission electron microscopy (TEM) to determine the major alterations in the microstructure of Candida (C.) albicans. The extract showed a favourable antimicrobial activity against C albicans with a minimum inhibitory concentration (MIC) value of 1.56 mg/mL. The main abnormalities noted from the SEM and TEM studies were the internal shrinkage of cell, disorganization within the cell cytoplasm and complete collapse of the yeast cells after 36 h of exposure to the extract. The time-kill assay suggested that the G. changii extract significantly inhibited C. albicans growth and it also exhibited prolonged antiyeast activity against the C albicans. The extract has shown in vitro fungicidal properties against C. albicans and should be investigated for its therapeutic potential.
  European review for medical and pharmacological sciences 09/2011; 15(9):1020-6. · 1.04 Impact Factor