Publications

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: (Full text is available at http://www.manu.edu.mk/prilozi). Examination of the composition of saliva in patients with diabetes may be useful for understanding why oral manifestations occur and how they should be treated. The purpose of this study was to determine the degree of severity of xerostomia, salivary concentrations of glucose and urea in patients with insulin-dependent diabetes, and to determine the correlation between xerostomia and salivary glucose levels. For the realization of this goal, the study included 60 patients of both sexes aged 30-70 years. The sample was divided into two groups. The first, experimental, group consisted of 30 patients who had insulin-dependent diabetes mellitus. The control group consisted of 30 subjects who were not suffering from diabetes. To determine the degree of severity of xerostomia among all respondents a questionnaire recommended by Carda was used. From all patients in both the control and experimental group, total saliva was collected for 10 minutes for biochemical analysis in accordance with the recommendations of Navazesh. Salivary glucose was determined by using the enzymatic method with a hexokinase (mmol/l), and salivary urea by using the kinetic method with urease and glutamate dehydrogenase (mmol/l). Varying degrees of xerostomia were noticed in 80% of the experimental group and only 10% of the control group. In diabetics, we found significantly higher levels of urea (2.36 mmol/l) and glucose (0.022 mmol/l) in the saliva compared with the values of these parameters (1.48 mmol/l, 0017 mmol/l) in the control group. Based on these results, we concluded that diabetes is a disease that causes xerostomia and there is a significant correlation between the degree of xerostomia and the salivary level of glucose. Key words: diabetes, saliva, urea, glucose.
    Prilozi / Makedonska akademija na naukite i umetnostite, Oddelenie za biološki i medicinski nauki = Contributions / Macedonian Academy of Sciences and Arts, Section of Biological and Medical Sciences 12/2012; 33(2):219-29.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study sought to evaluate the effect of low-level laser treatment combined with scaling and root planing (SRP) on gingival tissue levels of TNF-alpha in subjects with periodontal disease. Eighty gingival papilla biopsy samples were obtained from 60 patients diagnosed with chronic advanced periodontitis; randomly assigned to three treatment groups (n = 20), as well as 20 subjects with no periodontal disease (group A). Group B received SRP on a single quadrant/day for four consecutive days. On day 5, all quadrants were rescaled. Groups C and D received the same treatment as group B plus laser application with the low-level diode laser (630-670 nm, 1.875 J/cm(2)) for five and ten consecutive days, respectively. Papilla biopsies were obtained from subjects and evaluated by ELISA for levels of TNF-alpha. The values in the control group were 5.2 ± 3.21 pg/mg and baseline values for the examined groups were 46.01 ± 16.69. Significantly decreased level of TNF-alpha for groups C and D was found after treatment, while group B demonstrated reduction of TNF-alpha of 31.34%. The results of this study show suppression of TNF-alpha in gingival tissue after low-level laser treatment as adjunct to SRP. Data may suggest beneficial anti-inflammatory effects of the laser treatment when used as adjunctive periodontal treatment.
    Lasers in Medical Science 03/2011; 27(2):377-81. · 2.40 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study is to compare the effectiveness of low-level laser irradiation to traditional topical fluoride treatment for treatment choices of dentinal hypersensitivity following scaling and root planing. The experimental group (15 patients) was treated with low-energy-level diode laser at each site of dentinal hypersensitivity following scaling and root planning. The control group (15 patients) received topical fluoride treatment (protective varnish for desensitization). All the patients were treated at baseline visit, and then at day 2 and 4 after the initial treatment; the pain was subjectively assessed by the patients as strong, medium, medium low, low, or no pain. Total absence of the dental hypersensitivity was reported in 26.66% of the examined group even after the second visit, compared to the control group where complete resolution of the hypersensitivity was not present after the second visit in any of the treated cases. Complete absence of pain was achieved in 86.6% of patients treated with laser and only in 26.6% in the fluoride treated group, after the third visit. Based on our findings, we conclude that low-energy biostimulative laser treatment can be successfully used for treatment of dental hypersensitivity following scaling and root planing.
    Lasers in Medical Science 07/2009; 25(5):647-50. · 2.40 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study assessed the effects of low-level laser treatment in combination with scaling and root planing (SRP) in patients with periodontitis. Sixty subjects with chronic advanced periodontitis were assigned randomly to three treatment groups (n = 20) after collecting gingival clinical parameters. Group A received SRP on a single quadrant per day for four consecutive days; on the fifth day, all quadrants were rescaled. Group B received the same treatment as Group A, followed by laser application for five days. Group C received the same treatment as Group B but the laser treatment was administered for a total of 10 days. For Groups B and C, a low-level diode laser (630 to 670 nm) was used. The plaque index, gingival index, and sulcular bleeding index were recorded for all groups. For all clinical parameters, all three groups reported statistically significant differences (p < 0.005) compared to baseline data. Compared to Group A, Groups B and C showed statistically significant improvement for all clinical parameters. These findings suggest that a low-level diode laser can have a beneficial effect for treating inflammatory chronic advanced periodontitis.
    General dentistry 01/2009; 57(5):510-3.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Laser therapy is a standard therapeutic procedure, with unambiguous indications and contraindications. Among the reasons for this are: positive clinical experiences, scientifically verified objective changes in tissue equilibrium caused by laser, and above all, better understanding of the mechanisms of laser effects. Clinical and experimental experience shows that laser therapy has its greatest effects on cells/tissue/organs affected by a generally deteriorated condition with the ph value lower than normal. Biostimulation has been demonstrated with doses of laser emission from as low as 0,001 J/cm to 10 J/cm , with cytostatic effect above 240 J/cm . There were no macroscopic and microscopic damages to the tissue when recommended doses were used, which confirmed the safety of laser therapy. There are many indications for the laser use in oral pathology and periodontology, such as cheilitis exfoliativa sicca, gingivitis, gingivitis desquamativa, and periodontitis. Taking into consideration the effects of the laser treatment, such as activation of microcirculation, production of new capillaries and thickening of the capillary net, anti-inflammatory, analgesic effect, stimulated growth and regeneration of cells, and accelerated bone consolidation, following the need for incorporating non-invasive methods for minimizing the pain and discomfort either during or after dental treatment, we consider that the use of biostimulation laser therapy is an excellent treatment option.
    Acta Facultatis Medicae Naissensis 01/2006;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Focal infection can be defined as infections occurring in different locations of the human body and which are caused by microorganisms (or their products) inhabiting the oral cavity. The oral cavity is the site of many infectious and inflammatory disease , which has recently been associated with systemic diseases. Dental procedures (tooth extraction, endodontic treatment, root polishing etc), including periodontal therapy, can provoke the entrance of oral microorganisms of dental plaque into the blood flow and lymphatic system. This bacteriemia is short-term but in the conditions of weakened defense mechanisms, the risk of these bacteria is increased. In these cases, bacterial attacks on tissues and organs, after a while, lead to their multiplications and the origin of oral focal infections. Some diseases, such as infective endocarditis, infections of head and neck, respiratory infections, diseases of gastrointestinal tract, skin diseases, bone diseases and premature birth, occur as the consequence of the transmission of microbes fromdental foci. Many recent researches also list a periodontal disease as a risk factor. Chronic periodontitis is probably the most prevalent and potentially has the strongest epidemiological and plausible mechanistic associations with these systemic diseases.
    Acta Facultatis Medicae Naissensis. 01/2006;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Oral lichen planus (OLP) is an oral lesion with an enigmatic etiology. To explore the possibility of psycho-somatization, we evaluated the psychological personality profiles of OLP patients. Twenty patients with reticular; 20 with erosive form of OLP, and 25 controls were tested with the psychological Minnesota Multiphasic Personality Inventory (MMPI)-202 test. Eight clinical scales (hypochondriasis, depression, hysteria, psychopathic deviate, paranoia, psychasthenia, schizophrenia, and hypomania) as well as cortisol level, CD3, CD4, CD8, and CD16 markers by group were compared. Psychosomatization was evaluated by the use of internalization ratio (IR) Index. A characteristic MMPI profile was noted in the OLP groups with high IR index value. Significant differences among the groups were detected for cortisol, CD4, CD8, and CD16 counts. Mean values for hypochondriasis, depression, and hysteria were all significantly different with significantly higher mean scores for both reticular and erosive OLP subjects compared with controls. Prolonged emotive stress in many OLP patients may lead to psychosomatization and may contribute to the initiation and clinical expression of this oral disorder. Clinical significance: If additional research involving a larger and more diverse sample of patients confirms these findings, clinical trials will be needed to determine whether adjunctive psychological intervention provides a benefit in treating patients with OLP.
    Journal Of Clinical Periodontology 11/2005; 32(10):1034-40. · 3.69 Impact Factor

2 Following View all

22 Followers View all