Sivasankar Shanmuganathan

Questions and Answers (8) View all

• Answer added in Cancer Biology
  24 Is it possible to determine a protein as a cancer biomarker using only cell lines?
  By Dipon Das · KIIT University
  Sivasankar Shanmuganathan · Sankara Nethralaya

  The serum proteome is so complex and has 10^12 proteins and most of proteins are common to all disease conditions. Hence you need to go for primary ce... [more]

  The serum proteome is so complex and has 10^12 proteins and most of proteins are common to all disease conditions. Hence you need to go for primary cell culture of tumor tissues, identify some proteins, and evaluate the same from serum of patients Vs Controls for validation, using LC-MS/MS. And you need to have large sample size of patients and controls for the study. regards siva
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• Answer added in Cell Culture
  6 Stale medium for neutralization of trypsin
  By Clayton Axiak · University of Malta
  Sivasankar Shanmuganathan · Sankara Nethralaya

  i agree with darius. you can use 1-5% FBS or FCS containing fresh media, not 10 or 20% FBS containing media to save FBS. 

  i agree with darius. you can use 1-5% FBS or FCS containing fresh media, not 10 or 20% FBS containing media to save FBS.
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• Answer added in Microscopy
  11 Is it necessary to permeabilize the cells to study E-caherin by IF?
  By Sivasankar Shanmuganathan · Sankara Nethralaya
  Sivasankar Shanmuganathan · Sankara Nethralaya

  hi georg, ya i found the same using uniprot. i am thought of using 0.1% tir-x 100. and will try methanol fixation and 4%PAF fixation. thanks for the a... [more]

  hi georg, ya i found the same using uniprot. i am thought of using 0.1% tir-x 100. and will try methanol fixation and 4%PAF fixation. thanks for the ans, and you confirmed me that am in right path. thanks
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• Answer added in Microscopy
  11 Is it necessary to permeabilize the cells to study E-caherin by IF?
  By Sivasankar Shanmuganathan · Sankara Nethralaya
  Sivasankar Shanmuganathan · Sankara Nethralaya

  hi guys, thanks for the reply. I want to study the integrity of cells junction, so membrane staining is enough for me. Am using rabbit mAb, generated... [more]

  hi guys, thanks for the reply. I want to study the integrity of cells junction, so membrane staining is enough for me. Am using rabbit mAb, generated against synthetic peptide corresponding to sequence of Pro780 of human E-cadherin. I dont know where it is present? i.e on the surface or on cytoplasmic side.
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• Question asked in Microscopy
  11 Is it necessary to permeabilize the cells to study E-caherin by IF?
  IF for E-Cadherin 
  IF for E-Cadherin
  By Sivasankar Shanmuganathan · Sankara Nethralaya
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