Publications (12) View all
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Article: Identification of Mycobacterium avium genotypes with distinctive traits by combination of IS1245-based restriction fragment length polymorphism and restriction analysis of hsp65.
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ABSTRACT: One-hundred eight Mycobacterium avium isolates from pigs, humans, birds, and bovines were typed by the IS1245-based restriction fragment length polymorphism (RFLP) method and PCR-restriction enzyme analysis (PRA) of hsp65. Nine clusters of isolates showing more than 80% similarity in their RFLP profiles were detected. The largest cluster (cluster B) included 32 of 79 pig isolates (40.5%), 3 of 25 human isolates (12%), and 1 of 2 bovine isolates, comprising 33% of all isolates. The second largest cluster (cluster A) included 18 pig isolates (22.8%) and 6 human isolates (24%). Six smaller clusters included six pig isolates (clusters C and D), four and two human isolates (clusters E and F, respectively), two pig isolates (cluster I), and two pig isolates plus one bovine isolate and the avian purified protein derivative strain (cluster H). Cluster G represented the "bird-type" profile and included the bird isolate in this series, one pig isolate, plus reference strain R13. PRA revealed four allelic variants. Seventy-seven isolates were identified as M. avium PRA variant I, 24 were identified as M. avium PRA variant II, 6 were identified as M. avium PRA variant III, and 1 was identified as M. avium PRA variant IV. Except for three isolates from cluster B, each of the RFLP clusters was associated with a single PRA pattern. Isolates with unique (nonclustered) RFLP profiles were distributed between PRA variants I and II, and there was one unique isolate of PRA variant IV. These observations are consistent with divergent evolution within M. avium, resulting in the emergence of distinct lineages with particular competence to infect animals and humans.Journal of Clinical Microbiology 02/2003; 41(1):44-9. · 4.15 Impact Factor -
Article: Avaliação da virulência em hamsters (Mesocricetus auratus) de estirpes de Mycobacterium avium presentes na população de suínos do sul do Brasil
Braz. J. Vet. Res. Anim. Sci. 01/2002; 39(4):202-207. -
Article: Identificação dos fatores de risco associados à ocorrência de micobacterioses no sul do Brasil. Estudo caso-controle.
Arq Instit Biol. 01/2001; 68(2):19-22. -
Article: Time series evaluation of traumatic lesions and airsacculitis at one poultry abattoir in the state of São Paulo, Brazil (1996-2005).
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ABSTRACT: Ten year official condemnation records of one officially inspected poultry abattoir in state of São Paulo, Brazil, were analyzed. Seasonal and cyclical trends were analyzed in relation to traumatic lesions and airsacculitis, which were the most relevant official condemnation causes. Time series analysis of the records, seasonal indexes and moving averages was used to describe the adherence to the mathematical model and to offer preventive management strategies for the slaughterhouse industry. Although cause-effect relationships were not defined, some insight was given into the causal mechanisms that generated the series.Preventive Veterinary Medicine 03/2010; 94(3-4):231-9. · 2.05 Impact Factor -
Article: [Tuberculosis lymphadenitis in slaughtered swine from the State of São Paulo, Brazil: microscopic histopathology and demonstration of mycobacteria].
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ABSTRACT: As the occurrence of tuberculosis lymphadenitis in swine constitutes a public health risk, especially in immunosuppressed individuals, the distribution of tuberculoid lesions and the presence of microbacteria in lymph nodes and hepatic and muscular tissue in swine for slaughter, in the State of S. Paulo, SP (Brazil), in the period from 1993 to 1994, were studied. MATERIAL E METHOD: Tuberculous lesions in 60 carcasses of swine, slaughtered at abattoirs during the period 1993-1994, were studied. When macroscopic lesions were observed, a representative sampling of lymph nodes, hepatic and muscular tissues (masseter and diaphragm) were examined using histopathological and microbacterial isolation techniques. The macroscopic lesions were found predominantly in mesenteric lymph nodes. The MAI Complex (M.avium-intracellulare) was isolated mainly in the carcass group that showed lesions and was not found in the control group. Microorganisms of the MAI Complex were not isolated from hepatic and muscular tissues. No clear relationship between the type of mycobacteria isolated and the macroscopic lesions observed during the carcass inspection was found.Revista de Saúde Pública 09/1997; 31(4):391-7. · 1.33 Impact Factor
