Siğnem ONEY

Publications

• Chromosome counts and karyomorphology of some species of Artemisia L. (Asteraceae) from Turkey

  Selma TABUR, Şemsettin CİVELEK, Siğnem ÖNEY, Şadiye Betül YILMAZ ERGÜN, Murat KURŞAT, İsmail TÜRKOĞLU

  Turkish journal of Botany. 01/2012;

  Chromosome numbers and karyotypes of some species belonging to the genus Artemisia L. (Asteraceae) were investigated, and chromosome counts of 12 species, 1 of which was counted for the fi rst time, are presented with a thorough compilation of previously published data. All of the species studied be... [more] Chromosome numbers and karyotypes of some species belonging to the genus Artemisia L. (Asteraceae) were investigated, and chromosome counts of 12 species, 1 of which was counted for the fi rst time, are presented with a thorough compilation of previously published data. All of the species studied belong to the subgenus Artemisia Less. Chromosome numbers of 6 taxa confi rmed the previous data. Additionally, karyotypes of 8 species were reported. Karyotype analyses of 6 Artemisia species are reported here for the fi rst time. Th e basic chromosome numbers in the species studied were x = 8 and x = 9, with ploidy levels ranging from 2x to 4x and 6x. B chromosomes were also present in Artemisia incana (L.) Druce as 2n = 2x = 16 + 2B.

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• Genetic Characterization of New Brachypodium distachyon Populations from Diverse Geographic Regions in Turkey

  Metin TUNA, İlker NİZAM, Siğnem ÖNEY, Gülsemin Savaş, John Vogel

  First European Brachypodium Workshop; 10/2011

  The small annual grass Brachypodium distachyon is rapidly emerging as a powerful model system for temperate grasses due to its biological, physical and genomic characteristics. The natural range of B. distachyon largely overlaps the ancestral range of cultivated small grains and Turkey lies in the c... [more] The small annual grass Brachypodium distachyon is rapidly emerging as a powerful model system for temperate grasses due to its biological, physical and genomic characteristics. The natural range of B. distachyon largely overlaps the ancestral range of cultivated small grains and Turkey lies in the center of that geographical area thus granting a great amount of genetic variability. The objective of this study was to establish and genetically characterize a large B. distachyon germplasm collection originating from diverse geographical regions in Turkey. Seeds from approximately 20 individual plants were collected from each of the 118 locations throughout Turkey. Flow cytometry was used to determine the ploidy of representatives from each location. Seventy five of the populations contained only diploid plants, 35 of the populations contained only polyploid plants and 8 populations contained a mix of diploid and polyploid plants. To examine the genetic diversity of this new collection, a representative subset of 185 diploid individuals was genotyped using SSR markers. Our preliminary results show considerable genetic diversity both between and within populations. We also compared the SSR profiles of the new lines to previously examined lines. Significantly, some of the new accessions were contained in new clades indicating that we have not sampled Turkish B. distachyon diversity to saturation. Future plans for this collection include phenotypic characterization with an emphasis on biotic and abiotic stress. We also plan to develop recombinant inbred lines from divergent lines.

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• Chromosome numbers and karyotypes of some taxa of genus Artemisia (Asteraceae, Anthemideae) subgenus Dracunculus (Bess.) Rydb

  TABUR1, SELMA, ŞEMSETTIN CIVELEK, SIĞNEM ÖNEY, ŞADIYE BETÜL YILMAZand MURAT KURŞAT

  CARYOLOGIA. 01/2011; 64:335- 342.

  Chromosome numbers and karyotypes of four taxa of genus Artemisia subgenus Dracunculus were investigated. Chromosome numbers of three varieties (var. campestris, var. marschalliana and var. araratica) of Artemisia campestris were found as 2n=4x=36. In the present work, chromosome number of A. campes... [more] Chromosome numbers and karyotypes of four taxa of genus Artemisia subgenus Dracunculus were investigated. Chromosome numbers of three varieties (var. campestris, var. marschalliana and var. araratica) of Artemisia campestris were found as 2n=4x=36. In the present work, chromosome number of A. campestris var. araratica and detailed karyotypes of A. campestris var. marschalliana and A. scoparia are presented for the fi rst time. The chromosome complement of A. campestris var. marschalliana (2n=4x=36) consists of 28 median and eight submedian chromosomes. That of A. scoparia (2n=2x=16) consists of 16 median- centromeric chromosomes. We believe that these karyological data will enhance the karyological knowledge of subgenus Dracunculus and will prove to be an important source of information for new researches relating to genus.

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• DNA Content and Ploidy Determination of Brachypodium distachyon Populations Collected from Diverse Geographical Regions of Turkey by Flow Cytometer

  M. Tuna, Nizam, İ., S. Öney, G. Savaş, E.E Teykin

  9. The Plant Genomics Europian Meeting (Plant GEM), Istanbul/Turkiye; 01/2011

  Brachypodium is a small wild plant species emerging as a powerful model system for temperate grasses due to its biological, pysical and genomic chracteristics (rapid generation time, small genome, diploid ploidy level, small stature, simple growth requirements and self fertility).Brachypodium distac... [more] Brachypodium is a small wild plant species emerging as a powerful model system for temperate grasses due to its biological, pysical and genomic chracteristics (rapid generation time, small genome, diploid ploidy level, small stature, simple growth requirements and self fertility).Brachypodium distachyon is native to Mediterrianean region and it grows naturally in many regions of Turkey. However, the species morphologically similar diploid, tetraploid and hexaploid ecotips. Therefore, ploidy of Brachypodium distachyon plants needs to be determined before start using them in research projects. The objective of this study was to establish a Brachypodium distachyon germplasm collection with a wide natural variation by collecting seeds from diverse geographical regions of Turkey and distinguish diploid plants from polploid ones. Flow cytometer was used to determine DNA content of the plants. Mean DNA contents were correlated to ploidy level with root tip chromosome counts on a few selected plants whose DNA content indicated that they represented different groups. On the basis of DNA content, mean DNA content was 0.70 pg 2C-1 for diploids and 1.30 pg 2C-1 for tetraploids. Approximately 75 % of the plants determined to be diploid while 25 % of the plants determined to be tetraploid. In a few locations, diploids and tetraploids were observed in the same sampling area. Tetraploid plants were observed in mainly near the sea or hot and dry areas while diploids were observed in relatively more humid and fertile areas.

• ABA-Stimulators Antagonism on Mitotic Activity and Chromosomal Aberrations

  S. Tabur, S. Oney

  International Life Sciences Students' Conference, Kyiv/Ukraine; 08/2009

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• Effects of Some Artificial Fertilizers on Mitotic Index and Chromosome Behaviours in Vicia hybrida L. (Fabaceae).

  S. Tabur, S. Öney

  Journal Of Agricultural Research. 03/2009; 47:1-9.

  This study was conducted in Suleyman Demiral University, Arts and Sciences Faculty, Department of Biology, Isparta, Turkey during 2008 to determine whether ammonium sulphate (NH4)2SO4 and potassium sulphate (K2SO4) are genetically harmful or not. Ammonium sulphate and potassium sulphate were used as... [more] This study was conducted in Suleyman Demiral University, Arts and Sciences Faculty, Department of Biology, Isparta, Turkey during 2008 to determine whether ammonium sulphate (NH4)2SO4 and potassium sulphate (K2SO4) are genetically harmful or not. Ammonium sulphate and potassium sulphate were used as artificial fertilizer in various concentrations (1, 10, 50, 100, 250, 500 and 1000 μM) to see their effect on mitotic index and chromosome behaviour in Vicia hybrida. Levels of 1, 10 and 50 μM of both chemicals led to a significant increase in mitotic index. For example, mitotic index in root- tips of seeds germinated in distilled water was 0.16 while it was 0.15 at 1 μM, 0.20 at 10 μM and 0.25 at 50 μM in seeds germinated in media with (NH4)2SO4. In addition, mitotic index of seeds germinated in media with K2SO4 was 0.16 at seeds control, 0.17 at 1 μM, 0.18 at 10 μM and 0.23 at 50 μM. High concentrations caused gradual decreases in mitotic index. Various chromosome abnormalities were also observed in high concentrations.

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• Effects Of Chemıcal Fertılızers On Mıtotıc Index, Cell Cycle And Chromosome Behavıors In Vicia Faba L. Cv. Eresen- 87 Seeds Germınated Under Temperature Stress

  Siğnem ÖNEY

  01/2009

  Degree: M.Sc.

  Supervisor: Assist. Prof. Dr. Selma TABUR