Publications (101) View all
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Dataset: ACTA ANTI ACT OF DIFFERENT PARTS ANONA
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Article: Dentatin isolated from Clausena excavata induces apoptosis in MCF-7 cells through the intrinsic pathway with involvement of NF-kB signalling and G0/G1 cell cycle arrest: a bioassay-guided approach.
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ABSTRACT: ETHNOPHARMACOLOGICAL RELEVANCE: Clausena excavata Burm. f. has been used in folk medicines in eastern Thailand for the treatment of cancer. MATERIALS AND METHODS: To investigate the apoptosis mechanism, we isolated dentatin (DTN) from this plant using a bioassay-guided approach. DTN-induced cytotoxicity was observed with the MTT assay. Acridine orange/propidium iodide staining was used to detect cells in early apoptosis and high content screening (HCS) to observe nuclear condensation, cell permeability, mitochondrial membrane potential (MMP) and cytochrome c release. Apoptosis was confirmed with a clonogenic assay, DNA laddering and caspase 3/7 and 9 assays. Reactive oxygen species (ROS) formation, Bcl-2 and Bax expression, and cell cycle arrest were also investigated. The involvement of nuclear factor-kappa B (NF-kB) was analysed with the HCS assay. RESULTS: A significant increase in chromatin condensation in the cell nucleus was observed by fluorescence analysis. Apoptosis was confirmed by the reduced number of colonies in the clonogenic assay and the increased number of cellular DNA breaks in treated cells observed as a DNA ladder. Treatment of MCF-7 cells with DTN encouraged apoptosis with cell death-transducing signals that reduced MMP by down-regulation of Bcl-2 and up-regulation of Bax, triggering cytochrome c release from the mitochondria to the cytosol. The released cytochrome c triggered the activation of caspase 9 followed by the executioner caspase 3/7. DTN treatment significantly arrested MCF-7 cells at the G0/G1 phase (p<0.05) and ROS was significantly elevated. Moreover, DTN significantly blocked the induced translocation of NF-kB from cytoplasm to nucleus. CONCLUSION: Together, the results demonstrated that the DTN isolated from C. excavata inhibited the proliferation of MCF-7 cells, leading to cell cycle arrest and programmed cell death, which was confirmed to occur through the mitochondrial pathway with involvement of the NF-kB signalling pathway.Journal of ethnopharmacology 11/2012; · 2.32 Impact Factor -
Dataset: Anticancer and Antimicrobial Activities of Zerumbone from the Rhizomes of Zingiber zerumbut
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Dataset: Anticancer and Antimicrobial Activities of Zerumbone from the Rhizomes of Zingiber zerumbut
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Article: In vivo Antioxidative and Hepatoprotective Effects of Palm Date Fruits (Phoenix dactylifera)
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ABSTRACT: Palm date fruits have been used extensively for the traditional cure of liver illnesses and malaria in the Arab Peninsula. Therefore, the aim of the present study was to evaluate for the first time the in vivo hepatoprotective and the antioxidative effects of different varieties of palm date fruits in relation to their total phenolic contents and total flavonoids. This study was carried out using the CCl4-induced liver injuries in rabbits for two different periods: 4 and 20 h after CCl4-subcutaneous injection and oral administration of palm date syrups (5 g kg-1 b.wt.). The antioxidative and hepatoprotective effects of palm date syrups during the first and second experimental periods (4 and 20 h) were very clear, since results of plasma ALT and AST and TBARS (from liver homogenate) of the control were significantly higher than those obtained from animals treated with syrups. The present findings do strongly recommend to increase the consumption of palm date fruits, especially in cases of liver diseases and for the prevention of other serious diseases such as cardiovascular and cancers.International Journal of Pharmacology 03/2012; 8(3-1811-7775):185-191. · 1.50 Impact Factor
