Skills (10)
Research experience
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Jan 1997
Research: Universidade Federal do Rio de Janeiro
Universidade Federal do Rio de Janeiro · Departamento de VirologiaRio de Janeiro · Brazil
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Teaching: Clinical Virology.
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Teaching: Clinical Immunology
Other
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LanguagesEnglish, Portuguese.
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Scientific MembershipsSociedade Brasileira de Patologia Clinica, Sociedade Brasileira de Imuno-hematologia, International AIDS Society.
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Other InterestsAIDS, Hematology, JAMA, Science, Nature, Clinical Virolgy
Questions and Answers (20) View all
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Answer added in cDNA35 I'm having trouble with RT-PCR. What's the best method to quantify cDNA?Hi, how is the calibration of your micropipetes? Sometimes the problem is there or the way you are pippeting. You can find some answers to your questi... [more]
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Answer added in Molecular Diagnostics10 Why are my real time PCR amplification curves jagged regardless of using a SYBR green or TaqMan test?It seems that you have a problem of sample concentration. Have you a concentration experiment curve for samples and primers?
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Answer added in PCR34 Any recommendations for achieving an optimal standard curve using purified PCR product?All the comments above are valid, but in case you don't want to clone you can obtain a product using a High Fidelity Taq and then purifying it, this c... [more]
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Answer added in PCR26 Searching for a new real time PCR Machine - which one?I do not recommend Rotor Gene, its supplies are expensive specially the tubes for it. I also wouldn't buy something from Bio-Rad, as here in Brazil we... [more]
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Answer added in Cancer Biology13 MicroRNA 200 family in thyroid cancerHi Dr. Dhananjayan, in this case I think is important to verify if your primers and probes are good, specially if the primers are not forming dimmers.... [more]
Publications (1) View all
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Article: Rickettsiae-infected ticks in an endemic area of spotted fever in the State of Minas Gerais, Brazil
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ABSTRACT: A study on tick-borne rickettsiosis was developed in the county of Santa Cruz do Escalvado, State of Minas Gerais, Brazil, where a clinical case of the disease, confirmed by necropsy, had been reported. Of the 1,254 ticks collected, 1,061 belonged to the Amblyomma genus, 57 to the Rhipicephalus sanguineus species, 81 to Boophilus microplus, and 46 to Anocentor nitens. The hemolymph test associated with Gimenez staining showed that 18 of the 221 A. cajennense specimens, 1 of the 16 R. sanguineus, 1 of the 22 B. microplus, 3 of the A. nitens, and 1 of the A. ovale contained rickettsia-like microorganisms. Only 3 A. cajennense ticks were positive under direct immunofluorescence. A. cajennense was the only species found on humans.Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 92 Num 4. 01/1997;
