Santhoshkumar Thirunavukkarasu

Publications (23) View all

• Article: Larvicidal activity of green synthesized silver nanoparticles using bark aqueous extract of Ficus racemosa against Culex quinquefasciatus and Culex gelidus

  Kanayairam Velayutham, Abdul Abdul Rahuman, Govindasamy Rajakumar, Selvaraj Mohana Roopan, Gandhi Elango, Chinnaperumal Kamaraj, Sampath Marimuthu, Thirunavukkarasu Santhoshkumar, Moorthy Iyappan, Chinnadurai Siva
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  ABSTRACT: To investigate the larvicidal activity of synthesized silver nanoparticles (Ag NPs) utilizing aqueous bark extract of Ficus racemosa (F. racemosa) was tested against fourth instar larvae of filariasis vector, Culex quinquefasciatus (Cx. quinquefasciatus) and japanese encephalitis vectors, Culex gelidus (Cx. gelidus).
  Asian Pacific Journal of Tropical Medicine 02/2013; 6(2):95. · 0.37 Impact Factor
• Article: Larvicidal activity of green synthesized silver nanoparticles using bark aqueous extract of Ficus racemosa against Culex quinquefasciatus and Culex gelidus.

  Kanayairam Velayutham, Abdul Abdul Rahuman, Govindasamy Rajakumar, Selvaraj Mohana Roopan, Gandhi Elango, Chinnaperumal Kamaraj, Sampath Marimuthu, Thirunavukkarasu Santhoshkumar, Moorthy Iyappan, Chinnadurai Siva
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  ABSTRACT: To investigate the larvicidal activity of synthesized silver nanoparticles (Ag NPs) utilizing aqueous bark extract of Ficus racemosa (F. racemosa) was tested against fourth instar larvae of filariasis vector, Culex quinquefasciatus (Cx. quinquefasciatus) and japanese encephalitis vectors, Culex gelidus (Cx. gelidus). The synthesized Ag NPs was characterized by UV-vis spectrum, X-ray diffraction (XRD), Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR). The larvicidal activities were assessed for 24 h against the larvae of Cx. quinquefasciatus and Cx. gelidus with varying concentrations of aqueous bark extract of F. racemosa and synthesized Ag NPs. LC(50) and r(2) values were calculated. The maximum efficacy was observed in crude aqueous extract of F. racemosa against the larvae of Cx. quinquefasciatus and Cx. gelidus (LC(50)=67.72 and 63.70 mg/L; r(2)=0.995 and 0.985) and the synthesized Ag NPs (LC(50)=12.00 and 11.21 mg/L; r(2)=0.997 and 0.990), respectively. Synthesized Ag NPs showed the XRD peaks at 2 θ values of 27.61, 29.60, 35.48, 43.48 and 79.68 were identified as (210), (121), (220), (200) and (311) reflections, respectively. The FTIR spectra of Ag NPs exhibited prominent peaks at 3 425, 2 878, 1 627 and 1 382 in the region 500-3 000 cm(-1). The peaks correspond to the presence of a stretching vibration of (NH) C=O group. SEM analysis showed shape in cylindrical, uniform and rod with the average size of 250.60 nm. The biosynthesis of silver nanoparticles using bark aqueous extract of F. racemosa and its larvicidal activity against the larvae of disease spreading vectors. The maximum larvicidal efficacy was observed in the synthesized Ag NPs.
  Asian Pacific Journal of Tropical Medicine 02/2013; 6(2):95-101. · 0.37 Impact Factor
• Source

  Available from: Vishnu Kirthi

  Article: Novel and simple approach using synthesized nickel nanoparticles to control blood-sucking parasites.

  Govindasamy Rajakumar, Abdul Abdul Rahuman, Kanayairam Velayutham, Jeyaraman Ramyadevi, Kadarkaraithangam Jeyasubramanian, Arumugam Marikani, Gandhi Elango, Chinnaperumal Kamaraj, Thirunavukkarasu Santhoshkumar, Sampath Marimuthu, Abdul Abduz Zahir, Asokan Bagavan, Chidambaram Jayaseelan, Arivarasan Vishnu Kirthi, Moorthy Iyappan, Chinnadurai Siva
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  ABSTRACT: The present study was on assessment of the anti-parasitic activities of nickel nanoparticles (Ni NPs) against the larvae of cattle ticks Rhipicephalus (Boophilus) microplus and Hyalomma anatolicum (a.) anatolicum (Acari: Ixodidae), fourth instar larvae of Anopheles subpictus, Culex quinquefasciatus and Culex gelidus (Diptera: Culicidae). The metallic Ni NPs were synthesized by polyol process from Ni-hydrazine as precursor and Tween 80 as both the medium and the stabilizing reagent. The synthesized Ni NPs were characterized by Fourier transform infrared (FTIR) spectroscopy analysis which indicated the presence of Ni NPs. Synthesized Ni NPs showed the X-ray diffraction (XRD) peaks at 42.76°, 53.40°, and 76.44°, identified as 111, 220, and 200 reflections, respectively. Scanning electron microscopy (SEM) analysis of the synthesized Ni NPs clearly showed that the Ni NPs were spherical in shape with an average size of 150nm. The Ni NPs showed maximum activity against the larvae of R. (B.) microplus, H. a. anatolicum, A. subpictus, C. quinquefasciatus and C. gelidus with LC(50) values of 10.17, 10.81, 4.93, 5.56 and 4.94mg/L; r(2) values of 0.990, 0.993, 0.992, 0.950 and 0.988 and the efficacy of Ni-hydrazine complexes showed the LC(50) values of 20.35, 22.72, 8.29, 9.69 and 7.83mg/L; r(2) values of 0.988, 0.986, 0.989, 0.944 and 0.978, respectively. The findings revealed that synthesized Ni NPs possess excellent larvicidal parasitic activity. To the best of our knowledge, this is the first report on larvicidal activity of blood feeding parasites using synthesized Ni NPs.
  Veterinary Parasitology 09/2012; · 2.58 Impact Factor
• Article: Evaluation of stem aqueous extract and synthesized silver nanoparticles using Cissus quadrangularis against Hippobosca maculata and Rhipicephalus (Boophilus) microplus.

  Thirunavukkarasu Santhoshkumar, Abdul Abdul Rahuman, Asokan Bagavan, Sampath Marimuthu, Chidambaram Jayaseelan, Arivarasan Vishnu Kirthi, Chinnaperumal Kamaraj, Govindasamy Rajakumar, Abdul Abduz Zahir, Gandhi Elango, Kanayairam Velayutham, Moorthy Iyappan, Chinnadurai Siva, Loganathan Karthik, Kokati Venkata Bhaskara Rao
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  ABSTRACT: The present study was to determine the efficacies of anti-parasitic activities of synthesized silver nanoparticles (Ag NPs) using stem aqueous extract of Cissus quadrangularis against the adult of hematophagous fly, Hippobosca maculata (Diptera: Hippoboscidae), and the larvae of cattle tick, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Contact toxicity method was followed to determine the potential of parasitic activity. Twelve milliliters of stem aqueous extract of C. quadrangularis was treated with 88ml of 1mM silver nitrate (AgNO(3)) solution at room temperature for 30min and the resulting solution was yellow-brown color indicating the formation extracellular synthesis of Ag NPs. The synthesized Ag NPs were characterized with UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscope (FESEM) and energy dispersive X-ray (EDX) spectroscopy. The synthesized Ag NPs were recorded by UV-visible spectrum at 420nm and XRD patterns showed the nanoparticles crystalline in nature. FTIR analysis confirmed that the bioreduction of Ag((+)) ions to Ag NPs were due to the reduction by capping material of plant extract. FESEM image of Ag NPs showed spherical and oval in shape. By using the Bragg's Law and Scherrer's constant, the average mean size of synthesized Ag NPs was 42.46nm. The spot EDX analysis showed the complete chemical composition of the synthesized Ag NPs. The mortality obtained by the synthesized Ag NPs from the C. quadrangularis was more effective than the aqueous extract of C. quadrangularis and AgNO(3) solution (1mM). The adulticidal activity was observed in the aqueous extract, AgNO(3) solution and synthesized Ag NPs against the adult of H. maculata with LC(50) values of 37.08, 40.35 and 6.30mg/L; LC(90) values of 175.46, 192.17 and 18.14mg/L and r(2) values of 0.970, 0.992 and 0.969, respectively. The maximum efficacy showed in the aqueous extract, AgNO(3) solution and synthesized Ag NPs against the larvae of R. (B.) microplus with LC(50) values of 50.00, 21.72 and 7.61mg/L; LC(90) values of 205.12, 82.99 and 22.68mg/L and r(2) values of 0.968, 0.945and 0.994, respectively. The present study is the first report on antiparasitic activity of the experimental plant extract and synthesized Ag NPs. This is an ideal eco-friendly and inexpensive approach for the control of H. maculata and R. (B.) microplus.
  Experimental Parasitology 06/2012; 132(2):156-65. · 2.12 Impact Factor
• Source

  Available from: Dinkar Sahal

  Article: Antimalarial activities of medicinal plants traditionally used in the villages of Dharmapuri regions of South India.

  Chinnaperumal Kamaraj, Naveen Kumar Kaushik, Abdul Abdul Rahuman, Dinesh Mohanakrishnan, Asokan Bagavan, Gandhi Elango, Abdul Abduz Zahir, Thirunavukkarasu Santhoshkumar, Sampath Marimuthu, Chidambaram Jayaseelan, Arivarasan Vishnu Kirthi, Govindasamy Rajakumar, Kanayairam Velayutham, Dinkar Sahal
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  ABSTRACT: An ethnopharmacological investigation of medicinal plants traditionally used to treat diseases associated with fevers in Dharmapuri region of South India was undertaken. Twenty four plants were identified and evaluated for their in vitro activity against Plasmodium falciparum and assessed for cytotoxicity against HeLa cell line. This antimalarial in vitro study was planned to correlate and validate the traditional usage of medicinal plants against malaria. An ethnobotanical survey was made in Dharmapuri region, Tamil Nadu, India to identify plants used in traditional medicine against fevers. Selected plants were extracted with ethyl acetate and methanol and evaluated for antimalarial activity against erythrocytic stages of chloroquine (CQ)-sensitive 3D7 and CQ-resistant INDO strains of Plasmodium falciparum in culture using the fluorescence-based SYBR Green I assay. Cytotoxicity was determined against HeLa cells using MTT assay. Promising antiplasmodial activity was found in Aegle marmelos [leaf methanol extract (ME) (IC(50)=7 μg/mL] and good activities were found in Lantana camara [leaf ethyl acetate extract (EAE) IC(50)=19 μg/mL], Leucas aspera (flower EAE IC(50)=12.5 μg/mL), Momordica charantia (leaf EAE IC(50)=17.5 μg/mL), Phyllanthus amarus (leaf ME IC(50)=15 μg/mL) and Piper nigrum (seed EAE IC(50)=12.5 μg/mL). The leaf ME of Aegle marmelos which showed the highest activity against Plasmodium falciparum elicited low cytotoxicity (therapeutic index>13). These results provide validation for the traditional usage of some medicinal plants against malaria in Dharmapuri region, Tamil Nadu, India.
  Journal of ethnopharmacology 03/2012; 141(3):796-802. · 2.32 Impact Factor