Samrat Bose |
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MSc integrated biochemical-tec...
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Bangalore University
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Department of Bio-Chemistry
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51.18
Skills (9)
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3 Questions377 Followers
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291 Questions7745 Followers
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34 Questions5019 Followers
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30 Questions1490 Followers
Research experience
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Jan 2010–
Dec 2013Research: Yerevan Physics Institute
Yerevan Physics InstituteYerevan · Armenia -
Jan 1970–
Dec 2013Research: Washington State University
Washington State University · School of Mechanical and Materials EngineeringPullman · USA -
Jan 2012
Research: King's College Hospital NHS
King's College Hospital NHSLondon · United Kingdom -
Jan 2012
Research: Savannah State University
Savannah State UniversitySavannah · USA -
Jan 2011–
Dec 2012Research: Mercer University
Mercer UniversityAtlanta · USA -
Jan 2010–
Dec 2012Research: University of Bergen
University of Bergen · Department of Physics and TechnologyBergen · Norway -
Jan 2002–
Dec 2012Research: Max-Planck-Institut für Festkörperforschung
Max-Planck-Institut für FestkörperforschungStuttgart · Germany -
Jan 2000–
Dec 2012Research: Saha Institute of Nuclear Physics
Saha Institute of Nuclear PhysicsCalcutta · India -
Jan 1993–
Dec 2012Research: IIT Kharagpur
IIT Kharagpur · Department of Materials Science, Department of Electrical EngineeringKharagpur · India -
Jan 1991–
Dec 2012Research: Brock University
Brock University · Department of PhysicsSt. Catharines · Canada -
Jan 2009–
Dec 2011Research: California Institute of Technology
California Institute of TechnologyPasadena · USA -
Jan 2009–
Dec 2011Research: Memorial University Medical Center
Memorial University Medical CenterSavannah · USA -
Jan 2002–
Dec 2011Research: University College London
University College London · Department of Physics and AstronomyLondon · United Kingdom -
Jan 1997–
Dec 2011Research: University of California, San Francisco
University of California, San Francisco · Division of Hospital Medicine, Department of PediatricsSan Francisco · USA -
Jan 2010
Research: Chonbuk National University
Chonbuk National UniversitySeoul · South Korea -
Jan 2010
Research: University of Oslo
University of Oslo · Department of PhysicsOslo · Norway -
Jan 2007–
Dec 2010Research: Anna University, Chennai
Anna University, Chennai · Department of Computer Science and EngineeringChennai · India -
Jan 2006–
Dec 2010Research: Indian Institute of Technology Kanpur
Indian Institute of Technology Kanpur · Department of Physics, Department of Electrical EngineeringKānpur · India -
Jan 2007–
Dec 2008Research: University of Florida
University of Florida · Department of Physiology and Functional GenomicsGainesville · USA -
Jan 2007–
Dec 2008Research: Tata Institute of Fundamental Research
Tata Institute of Fundamental ResearchMumbai · India -
Jan 1974–
Dec 2008Research: University of Calcutta
University of Calcutta · Department of BiochemistryCalcutta · India -
Jan 2007
Research: University of Cambridge
University of CambridgeCambridge · United Kingdom -
Jan 2007
Research: Arizona State University
Arizona State UniversityTempe · USA -
Jan 2004–
Dec 2007Research: Drexel University
Drexel University · Department of PhysicsPhiladelphia · USA -
Jan 2006
Research: Hanyang University
Hanyang UniversityAnsan · South Korea -
Jan 2006
Research: University of California, Irvine
University of California, Irvine · Department of OphthalmologyIrvine · USA -
Jan 2006
Research: University of Birmingham
University of BirminghamBirmingham · United Kingdom -
Jan 2006
Research: University of California, Santa Cruz
University of California, Santa CruzSanta Cruz · USA -
Jan 2004–
Dec 2006Research: Weizmann Institute of Science
Weizmann Institute of Science · Department of Biological RegulationIsrael -
Jan 2004
Research: Medical College Kolkata
Medical College KolkataCalcutta · India -
Jan 2003–
Dec 2004Research: University of Western Australia
University of Western Australia · School of Chemistry and BiochemistryPerth · Australia -
Jan 2003
Research: Memorial University of Newfoundland
Memorial University of Newfoundland · Department of BiologySaint John · Canada -
Jan 2002
Research: Max-Planck-Institut für Gravitationsphysik (Albert-Einstein-Institut)
Max-Planck-Institut für Gravitationsphysik (Albert-Einstein-Institut)Potsdam · Germany -
Jan 2001–
Dec 2002Research: University of Oxford
University of OxfordOxford · United Kingdom -
Jan 1997–
Dec 2002Research: Imperial College London
Imperial College London · Section of Statistics, Centre for HaematologyLondon · United Kingdom -
Jan 2001
Research: Harbor-UCLA Medical Center
Harbor-UCLA Medical CenterTorrance · USA -
Jan 2001
Research: Auburn University
Auburn University · Department of Pharmacal SciencesAuburn · USA -
Jan 2001
Research: University of California, Los Angeles
University of California, Los Angeles · Department of Pathology and Laboratory MedicineLos Angeles · USA -
Jan 2001
Research: The Catholic University of America
The Catholic University of AmericaWashington, D. C. · USA -
Jan 1999–
Dec 2001Research: University of Bristol
University of Bristol · School of BiochemistryBristol · United Kingdom -
Jan 1995–
Dec 2001Research: Technische Universität Berlin
Technische Universität Berlin · Department of Theoretical PhysicsBerlin · Germany -
Jan 1991–
Dec 2001Research: Postgraduate Institute of Medical Education and Research
Postgraduate Institute of Medical Education and Research · Department of HepatologyChandīgarh · India -
Jan 1996–
Dec 1999Research: National Heart, Lung, and Blood Institute
National Heart, Lung, and Blood InstituteBethesda · USA -
Jan 1998
Research: Minneapolis Veterans Affairs Hospital
Minneapolis Veterans Affairs HospitalMinneapolis · USA -
Jan 1997–
Dec 1998Research: Columbia University
Columbia University · College of Physicians and SurgeonsNew York City · USA -
Jan 1997
Research: Universität Regensburg
Universität Regensburg · Institut für Biophysik und physikalische BiochemieRegensburg · Germany -
Jan 1997
Research: Bharati Vidyapeeth Deemed University
Bharati Vidyapeeth Deemed University · Department of Electrical EngineeringPune · India -
Jan 1997
Research: Indraprastha Apollo Hospitals
Indraprastha Apollo HospitalsNew Delhi · India -
Jan 1997
Research: Julphar School of Pharmacy
Julphar School of PharmacyDenver · USA -
Jan 1995–
Dec 1997Research: Medical College of Wisconsin
Medical College of Wisconsin · Department of Biochemistry, Department of MedicineMilwaukee · USA -
Jan 1988–
Dec 1997Research: All India Institute of Medical Sciences
All India Institute of Medical Sciences · Department of Anatomy, Department of Pathology, Department of Obstetrics and GynaecologyNew Delhi · India -
Jan 1996
Research: Jadavpur University
Jadavpur University · Department of Electrical EngineeringCalcutta · India -
Jan 1996
Research: Memorial Sloan-Kettering Cancer Center
Memorial Sloan-Kettering Cancer Center · Department of PathologyNew York City · USA -
Jan 1995–
Dec 1996Research: University of California, Davis
University of California, Davis · Department of Electrical and Computer EngineeringDavis · USA -
Jan 1993–
Dec 1996Research: Visva Bharati University
Visva Bharati University · Department of ZoologyBolpur · India -
Jan 1992–
Dec 1996Research: Cornell University
Cornell University · Department of Electrical and Computer EngineeringIthaca · USA -
Jan 1995
Research: New York University USA
New York University USANew York City · USA -
Jan 1995
Research: National Institutes of Health
National Institutes of HealthBethesda · USA -
Jan 1995
Research: University of Pennsylvania
University of Pennsylvania · Department of OphthalmologyPhiladelphia · USA -
Jan 1992–
Dec 1995Research: University of Kent
University of Kent · Biological LaboratoryCanterbury · United Kingdom -
Jan 1992–
Dec 1995Research: AT&T Labs
AT&T LabsAustin · USA -
Jan 1994
Research: Lankenau Institute for Medical Research
Lankenau Institute for Medical ResearchWynnewood · USA -
Jan 1993–
Dec 1994Research: Louisiana State University
Louisiana State University · Department of Computer ScienceBaton Rouge · USA -
Jan 1991–
Dec 1994Research: University of Colorado Denver
University of Colorado DenverDenver · USA -
Jan 1979–
Dec 1992Research: Washington University in St. Louis
Washington University in St. LouisSaint Louis · USA -
Jan 1991
Research: Sriram Chandra Bhanj Medical College & Hospital
Sriram Chandra Bhanj Medical College & HospitalCuttack · India -
Jan 1991
Research: Queen's University Belfast
Queen's University BelfastBelfast · United Kingdom -
Jan 1969–
Dec 1991Research: Chittaranjan National Cancer Institute
Chittaranjan National Cancer InstituteCalcutta · India -
Jan 1988
Research: Johns Hopkins University
Johns Hopkins University · Department of PediatricsBaltimore · USA -
Jan 1988
Research: Lady Hardinge Medical College
Lady Hardinge Medical CollegeNew Delhi · India -
Jan 1979
Research: Indiana University Bloomington
Indiana University Bloomington · Department of ChemistryBloomington · USA -
Jan 1977–
Dec 1978Research: Govt. Model Science College, Jabalpur
Govt. Model Science College, JabalpurJabalpur · India -
Jan 1974
Research: National Institute of Mental Health (NIMH)
National Institute of Mental Health (NIMH)Bethesda · USA
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May 2006
Research: PREPARING DNA TEMPLATE NANO GOLD PARTICLE
IIT-DELHI · NANOTECHNOLOGY · IIT-DELHIDELHI
Education
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Jun 2007–
Jul 2009Bangalore University
BIO-CHEMICAL ENGINEERING · MASTER DEGREEIndia · BANGALORE
Other
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LanguagesENGLISH,.....HINDI,,,BENGALI,,,SANSKRIT
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Scientific MembershipsNASA,IAEA,DOAE
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Other InterestsPLAYING CRICKET AND VOLLEYBALL...AND CHESS(INDOOR)
Questions and Answers (12) View all
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Answer added in Nanobiotechnology4 Solubility of egg albuminBy Rajeev Sharma · Roorkee Engineering & Management Technology InstituteSamrat Bose · Bangalore UniversityInfluence of different pH values (3.0, 4.6, 6.0, 8.0 and 9.0) and salt concentrations (0.05, 0.1, 0.2, 0.35 and 0.5 mol/l) for three types of salt (Na... [more]Influence of different pH values (3.0, 4.6, 6.0, 8.0 and 9.0) and salt concentrations (0.05, 0.1, 0.2, 0.35 and 0.5 mol/l) for three types of salt (NaCl, Na2SO4 and (NH4)2SO4) on the solubility of egg white protein and density of egg white, at room temperature (25 °C) was studied. The results showed that the solubility of egg white protein was influenced by pH as well as concentration and type of salt present in the medium. Protein solubility increased with increase in pH, with higher solubility showed at pH 9.0 and lower solubility at pH 4.6. This behavior was verified for all the salts analysed. At acid pH (pH 3.0), it was observed the tendency of solubility elevation on the increase of the saline concentration, due to the salting-in effect. The density increased with increase in the salt concentration.Following
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Answer added in Biotechnology29 Do proteins precipitate during dialysis?By Shivakiran Makam · Defence Research and Development OrganisationSamrat Bose · Bangalore UniversityProtein precipitation during dialysis against phosphate or Tris buffers (PBS or TBS) can be often caused by: too low salt concentration (NaCl, KCl... [more]Protein precipitation during dialysis against phosphate or Tris buffers (PBS or TBS) can be often caused by: too low salt concentration (NaCl, KCl) too high protein concentration sudden pH changes pH of dialysis buffer close to PI of a protein Therefore: use at least 1000 fold excess of dialysis buffer perform procedure at 4c(at least 3 hours, followed by a buffer change) use at least 350 mM salt in the dialysis buffer optional: 50 % glycerol Precipitated proteins are very difficult to refold. If possible purify your protein again and apply other conditions for dialysisFollowing
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Answer added in Transformation (Genetics)4 Topo cloningBy Claudia Anthony · University of MalayaSamrat Bose · Bangalore UniversityCheck via through invitrogen process... Catalytic process should be in ur favor,,because the kinetic complexity increases in in ampicillin....or use a... [more]Check via through invitrogen process... Catalytic process should be in ur favor,,because the kinetic complexity increases in in ampicillin....or use another effective anti-biotic....Following
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Answer added in Biotechnology29 Do proteins precipitate during dialysis?By Shivakiran Makam · Defence Research and Development OrganisationSamrat Bose · Bangalore UniversityDo one thing ...elute the .Protein in 20mM Tris, pH 7.9, 500 mm Na-Cl and 1M imidazole. Another protein, size 32kD is eluted in same buffer but with... [more]Do one thing ...elute the .Protein in 20mM Tris, pH 7.9, 500 mm Na-Cl and 1M imidazole. Another protein, size 32kD is eluted in same buffer but with 250 mM imidazole...if the ionic strength of electrolyte should be low....check the kinetic behavior of electrolyte.......check the pH level,,,,calibration should be there.......Following
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Answer added in Methods3 Experience/knowledge of sequencing FFPE-derived DNA?By Johanna Sandgren · Karolinska InstituteSamrat Bose · Bangalore UniversityAny WGA method to be applied to formalin-fixed DNA must be able to amplify relatively short lengths of nucleic acid stretches, ~1kb or less. Four WGA ... [more]Any WGA method to be applied to formalin-fixed DNA must be able to amplify relatively short lengths of nucleic acid stretches, ~1kb or less. Four WGA methods have been described and are in common use – they include linker adapter PCR (LAP)8-10, primer extension preamplification PCR (PEP)11, degenerate oligonucleotide PCR (DOP)12,13 and multiple strand displacement (MSD).14,15 While all have been successful in varying degrees when used with FFPE DNA, MSD has suffered in that it relies on a highly processive polymerase and high quality DNA chains to create long amplicons (>10kb). Because FFPE DNA is often comprised of short pieces of DNA with amplification terminating lesions, this type of input DNA has been amplified with best representation by using LAP and DOP WGA .10,13,16 GenomePlex® uses a modified DOP amplification to effect whole genome amplification, and the method has been successfully applied to formalin-fixed samples.16 There has been some confusion as to the mechanism of this system, which was first described in a 2004 Sigma-Aldrich Life Science Quarterly article.17 GenomePlex uses a mild fragmentation step to randomly break up long DNA, which facilitates subsequent steps. Priming is accomplished using an oligo with known 5’ ends but degenerate 3’- ends, and the multicopy DNA sample is reproduced in a series of overlapping single stranded DNAs. These single stranded products are subsequently primed with the same oligo to create double stranded DNA with fixed 5’- ends. The resulting library is amplified using PCR,Following
Publications (1066) View all
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Article: Acute sporadic non‐A, non‐B viral hepatitis of adults in India—Epidemiological and immunological studies
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ABSTRACT: On the basis of tests for specific IgM class antibodies in the serum, infections by hepatitis A virus, hepatitis B virus and hepatitis non-A, non-B virus accounted for 7%, 37.8% and 54.2% respectively of 286 cases of acute sporadic viral hepatitis in adults from three different regions of India. Epstein-Barr virus and cytomegalo virus were insignificant (0.5–1%) aetiological agents. Approximately one-quarter of all non-A, non-B (NANB) cases were additionally HBsAg carriers; there were significant differences in regional frequencies. NANB hepatitis commonly affected individuals in the mid-fourth decade and occurred throughout the year with small peaks in different seasons. No antigen-antibody reaction could be demonstrated in various immunological tests including radioimmunobinding using acute and convalescent phase sera. Neither were virus-like particles visualized in acute phase sera on electron and immuno-electron microscopy. However, rhesus monkeys inoculated with acute phase serum from a case of NANB hepatitis developed distinct hepatocellular changes at 43–55 days after inoculation, which had some features of similarity with experimental NANB hepatitis of other simian species. Sporadic NANB hepatitis is a major health problem throughout India and intensive study is needed on its epidemiology and aetiology.Journal of Gastroenterology and Hepatology 03/2008; 2(4):333 - 345. · 2.87 Impact Factor -
Article: Malabsorption in thyroid dysfunctions.
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ABSTRACT: Studies of intestinal absorptive functions in 11 hyperthyroid and 14 hypothyroid patients showed that steatorrhoea was common in hyperthyroid patients and rare in hypothyroid patients. D-xylose malabsorption was present in both the groups of thyroid disorders being more common in hypothyroid one. Occasionally vitamin B12 malabsorption was found in 2 hypothyroid patients. Jejunal mucosal changes were almost unremarkable. Both fat and carbohydrate malabsorption in both groups of patients appeared to be casually related to the thyroid dysfunction.Journal of the Indian Medical Association 08/1991; 89(7):195-7. -
Article: Early onset duodenal ulcer with special reference to serum alpha-1-antitrypsin activity.
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ABSTRACT: Thirteen patients of duodenal ulcer, below 20 years of age, ten age and sex matched controls and 17 first probands were studied to find out (i) the special characteristics of this duodenal ulcer subtype and (ii) the serum alpha-1-antitrypsin (alpha-1-AT) activity in these patients and their first probands. Basal and maximal acid output, and serum level of alpha-1-AT were measured, and serum agar gel electrophoresis done, in the above subjects. Special characteristics like positive family history, and high basal (p less than or equal to 0.01) and maximal (p less than or equal to 0.001) acid output were noted in these patients. Serum alpha-1-AT was found to be deficient in nine patients. Five first probands also had the heterozygous level of the enzyme deficiency. Thus, patients of early onset duodenal ulcer seem to have special characteristics with alpha-1-antitrypsin deficiency in them as well as in their first probands.The Journal of the Association of Physicians of India 03/1991; 39(2):182-4. -
Article: A clinico-radiological reappraisal of intestinal tuberculosis--changing profile?
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ABSTRACT: Intestinal tuberculosis is still common in developing countries. In 186 patients with intestinal tuberculosis, clinical features, radiological findings and complications were carefully recorded and compared with those from earlier studies with a view to study any possible changes after the liberal use of antitubercular drugs. Sixty two percent of the patients in the present series had had prior exposure to antitubercular drugs. The incidence of systemic symptoms like fever and anorexia, alternating diarrhoea and constipation, peritoneal and lymph node involvements and associated pulmonary lesions were less frequently observed. On the other hand, an indolent and complicated course with intestinal obstruction (47%) and lower gastrointestinal bleeding (5.5%) and frequent colonic involvement (19%) often necessitating surgical intervention appeared to have become more frequent than reported in earlier series. Awareness of these changes in the clinical profile of intestinal tuberculosis should be helpful in the diagnosis and management of the condition.Gastroenterologia Japonica 03/1986; 21(1):17-22. -
Article: Peritoneoscopy in undiagnosed ascites.
The Journal of the Association of Physicians of India 09/1985; 33(8):527-8.