Sameer Sathaye

Inos Technologies, Ann Arbor · Corporate R&D

Rheological characterization of physically crosslinked peptide‐ and protein‐based hydrogels is widely reported in the literature. In this review, we focus on solid injectable hydrogels, which are commonly referred to as ‘shear‐thinning and rehealing’ materials. This class of what sometimes also are called ‘yield‐stress’ materials holds exciting pro...

MAX1 β-hairpin peptide (VKVKVKVK-VDPPT-KVKVKVKV-NH2) has been shown to form nanofibrils with a cross-section of two folded peptides collapsed together with a valine-rich core and polymerized into a fibril primarily through hydrogen bonding1-7. These nanofibrils form hydrogel networks through fibril entanglements as well as fibril branching8. New β-...

There is intense interest in developing novel methods for the sustained delivery of low levels of clinical therapeutics. MAX8 is a peptide-based beta-hairpin hydrogel that has unique shear thinning properties that allow for immediate rehealing after the removal of shear forces, making MAX8 an excellent candidate for injectable drug delivery at a lo...

Polymers such as poly(N-vinyl-2-pyrrolidone) (PVP) have been used to prepare hydrogels for wound dressing applications but are not inherently bioactive. For enhanced healing, the release of physically admixed therapeutics from hydrogels has been evaluated, but with limited control over drug release profiles. To overcome these limitations, PVP was b...

Polymers such as poly(N-vinyl-2-pyrrolidone) (PVP) have been used to prepare hydrogels for wound dressing applications but are not inherently bioactive. For enhanced healing, PVP was blended with salicylic acid-based poly(anhydride-esters) (SAPAE) and shown to exhibit hydrogel properties upon swelling. In vitro release studies demonstrated that the...

Hydrophobic collapse of amphiphilic β-hairpin peptides (e.g. MAX1 VKVKVKVKV^DPPTKVKVKVKV-NH2) into fibrils and their hierarchical assembly into branched, hydrogel networks has been extensively studied. A physically crosslinked hydrogel network is formed due to fibrillar entanglement and branched defects in hydrophobic collapse during fibril formati...

We studied diffusion profiles of varying hydrophobicity drugs in a beta-hairpin peptide hydrogel solid that is shear thinning, injectable, and immediate reheals after shear. These rheological properties result from its entangled and branched fibrillar nanostructures, formed from intrmolecular folding and consequent intermolecular assembly of the pe...

Hydrogels are quickly becoming an important biomaterial that can be used for the safe, localized injection of cancer drugs, the injection of stem cells into areas of interest or other biological applications. Our peptides can be self-assembled in a syringe where they form a gel, sheared by injection and, once in the body, immediately reform a local...

Hydrophobic collapse of amphiphilic -hairpin peptides (e.g. MAX1 VKVKVKVKV^DPPTKVKVKVKV-NH2) into fibrils and their hierarchical assembly into branched, hydrogel networks has been extensively studied. A physically crosslinked hydrogel network is formed due to fibrillar entanglement and branched defects in hydrophobic collapse during fibril formatio...

Nanoscale fibril self-assembly in (VK)4VDPPT (KV)4-NH2 peptides is initiated by intramolecular peptide folding into a beta-hairpin conformation. Once folded, the peptides undergo intermolecular bilayer formation due to hydrophobic collapse of hydrocarbon valine side chains, all located on one face of respective folded beta-hairpins. Subsequent inte...

Hybrid Gels constitute a novel class of polymeric materials developed with an aim of combining and/or enhancing the diverse and complementary properties of their individual constituent networks. Self-assembling peptide hydrogels formed from aqueous solutions of beta-hairpin forming peptides have been extensively reported. These hydrogels are intere...