Sadiq Umar

Publications (17) View all

• Article: Quercetin Prevents Protein Nitration and Glycolytic block of Proliferation in Hydrogen Peroxide insulted cultured Neuronal Precursor Cells (NPCs): Implications on CNS Regeneration.

  Mir Sajad, Jamil Zargan, Mohammad Afzal Zargar, Jyoti Sharma, Sadiq Umar, Rajesh Arora, Haider A Khan
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  ABSTRACT: Survival along with optimal proliferation of neuronal precursors determines the outcomes of the endogenous cellular repair in CNS. Cellular-oxidation based cell death has been described in several neurodegenerative disorders. Therefore, this study was aimed at the identification of the potent targets of oxidative damage to precursors and its effective prevention by a natural flavanoid, Quercetin. Neuronal precursor cells (NPCs), Nestin+ and GFAP (Glial fibrillary acidic protein)+were isolated and cultured from adult rat SVZ (subventricular zone).These cells were challenged with a single dose of H(2)O(2) (50μM) and/or pre-treated with different concentrations of Quercetin. H(2)O(2) severely limited the cellular viability and expansion of the neurospheres. Cellular-oxidation studies revealed reduction in glutathione dependent redox buffering along with depletion of enzymatic cellular antioxidants that might potentiate the nitrite (NO(2)(-)) and superoxide anion (O(2)(-)) mediated peroxynitrite (ONOO(-)) formation and irreversible protein nitration. We identified depleted PK-M2 (M2 isoform of pyruvate kinase) activity and apoptosis of NPCs revealed by the genomic DNA fragmentation and elevated PARP (poly ADP ribose polymerase) activity along with increased Caspase activity initiated by severely depolarised mitochondrial membranes. However, the pre-treatment of Quercetin in a dose response manner prevented these changes and restored the expansion of neurospheres preferably by neutralizing the oxidative conditions and thereby reducing peroxynitrite formation, protein nitration and PK-M2 depletion. Our results unravel the potential interactions of oxidative environment and respiration in the survival and activation of precursors and offer a promise shown by a natural flavanoid in the protective strategy for neuronal precursors of adult brain.
  NeuroToxicology 02/2013; · 3.10 Impact Factor
• Article: Protective effect of rutin in attenuation of collagen-induced arthritis in Wistar rat by inhibiting inflammation and oxidative stress

  Sadiq Umar, Navin Kumar Mishra, Kaushal Pal, Mir Sajad, Neha, Md. Meraj Ansari, Sayeed Ahmad, Chandra K. Katiyar, Haider A. Khan
  Indian Journal of Rheumatology 11/2012;
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  Available from: Sadiq Umar

  Article: NEPHROPROTECTIVE ACTION OF PEUCEDANUM GRANDE AGAINST CADMIUM CHLORIDE INDUCED RENAL TOXICITY IN WISTAR RATS

  Mohammad Aslam, Shiekh Tanveer Ahmad, Rameshver Dayal, Kalim Javid, Sadiq Umar, Asia Asiaf, Sana Nafees, Jalal Uddin Bhat, Arjumand Wani, Mohammad Samim, Surender Singh
  EXCLI Journal 08/2012; 11:444-52. · 1.06 Impact Factor
• Article: Hesperidin inhibits collagen-induced arthritis possibly through suppression of free radical load and reduction in neutrophil activation and infiltration.

  Sadiq Umar, Anubhav Kumar, Mir Sajad, Jamil Zargan, Md Meraj Ansari, Sayeed Ahmad, Chandra Kant Katiyar, Haider A Khan
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  ABSTRACT: Rheumatoid arthritis is a chronic inflammatory disease characterized by the destruction of articular cartilage and bone in a chronic phase. Pathology of rheumatoid arthritis suggests autoimmunity linked to inflammation. In our study, rheumatoid arthritis was induced in Wistar rats by intradermal injections of 100 μl of emulsion containing bovine type II collagen in complete Freund's adjuvant at the base of the tail. Disease developed about 13 ± 1 days after immunization and treatment with hesperidin (HES) at a dose of 160 mg kg(-1) body weight was given after onset of disease daily until 20th day. The effect of treatment in the rats was monitored by clinical scoring, biochemical parameters and histological evaluations in joints. A steady increase in the articular elastase, nitric oxide and lipid peroxidation was observed in joints of arthritic rats as compared to control, whereas a significant decrease in reduced glutathione, superoxide dismutase activity and catalase was observed in collagen-induced arthritis rats as compared to control group. The results from the present work indicate that the treatment with hesperidin was effective in bringing about significant changes on all the parameters studied in collagen-induced arthritis rats. These data confirm that erosive destruction of the joint cartilage in collagen-induced arthritis is due free radicals released by activated neutrophils and produced by other biochemical pathways. In the present study, an attempt has been made to amelioration of the disease process by a natural product. These results suggest that oral administration of HES could be effective for treating human RA patients.
  Rheumatology International 04/2012; · 1.88 Impact Factor
• Article: Modulation of the oxidative stress and inflammatory cytokine response by thymoquinone in the collagen induced arthritis in Wistar rats.

  Sadiq Umar, Jamil Zargan, Khalid Umar, Sayeed Ahmad, Chandra Kant Katiyar, Haider A Khan
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  ABSTRACT: Thymoquinone (TQ) is the major active compound derived from Nigella sativa. Our aim of this work was to evaluate the antioxidant and antiarthritic activity of TQ in Wistar rat by collagen induced arthritis (CIA). TQ was administered at a dose of 5mgkg(-1) body weight once daily for 21days. The effects of treatment in the rats were assessed by biochemical (articular elastase, MPO, LPO, GSH, catalase, SOD and NO), inflammatory mediators (IL-1β, IL-6, TNF-α, IL-10, IFN-γ and PGE(2)) and histological studies in joints. TQ was effective in bringing significant changes on all the parameters (articular elastase, MPO, LPO, GSH, catalase, SOD and NO) studied. Oral administration of TQ resulted in significantly reduced the levels of pro-inflammatory mediators (IL-1β, IL-6, TNF-α, IFN-γ and PGE(2)) and increased level of IL-10. The protective effects of TQ against RA were also evident from the decrease in arthritis scoring and bone histology. In conclusion, the fact that TQ abolished a number of factors known to be involved in RA pathogenesis indicates that the administration of thymoquinone may have potential value in the treatment of inflammatory disease.
  Chemico-biological interactions 03/2012; 197(1):40-6. · 2.46 Impact Factor