Skills (52)
Research experience
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Teaching: teach thyself :P
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Jun 2011–
Nov 2011Research: Occupational trainee
Australian National University · Genome Biology · Australian National UniversityComputational Proteomics · Canberra -
Jan 2010–
Dec 2010Research: Molecular Biologist
Swapnojaatra · SwapnojaatraSwapnajaatra work station -
Jun 2009–
Aug 2009Research: Research fellow
Bangladesh Council of Scientific researchIFST -
Aug 2008
Research: Research Associate
University of Dhaka · Biochemistry and Molecular Biology · University of DHakaPlant Biotechnology laboratory · Dhaka -
Jan 2008
Research: University of Dhaka
University of DhakaBangladesh · Dhaka -
Jun 2007–
Aug 2008Research: Research Student
Universiyt of Dhaka · Biochemistry and Molecular Biology · Universiyt of DhakaPlant Biotechnology Laboratory · Dhaka
Education
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Oct 2011
ANU
Gene Technology CourseBangladesh · Canberra -
Aug 2011
ANU
Chemical and Biological Safety courseAustralia · Canberra -
Nov 2009
Manilla Hotel
Rice Annotation Project 6 (RAP6) workshopManila -
Nov 2008
BRRI
GCP training workshop on Marker-assisted Breeding for BangladeshBangladesh · Gazipur -
May 2007–
May 2008University of Dhaka
Biochemistry and Molecular Biology · Masters of ScienceBangladesh · Dhaka -
May 2002–
Jun 2007University of Dhaka
Biochemistry and Molecular Biology · BSc (hons.)Bangladesh · Dhaka
Awards & achievements
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Nov 2009Scholarship: • Participation with accomodation Grant from IRRI and travel grant from USDA to participate in the 6th International Rice Genetics Symposium
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Jun 2009Scholarship: • Khandakar Mukaram Hossain Research fellowship by Bangladesh Council of Scientific and Industrial Research (BCSIR),
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Aug 2006Scholarship: • Rokeya Hall Scholarship for excellent result in BSc, 2005
Other
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LanguagesBangla, English
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Scientific MembershipsGraduate Biochemist Association, Global Network of Bangladeshi Biotechnologist
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Other Interestssurfing, listening music, painting, reading, PLoS, GM crop, Dan Browns... Biggyani shafdar alir moha moha abishkar
Publications (10) View all
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Book: Bongshogotibidyar mulkotha o gene prokoushol
01/2011; Scholars Publication. -
Article: Physiology and gene expression of the rice landrace Horkuch under salt stress
[show abstract] [hide abstract]
ABSTRACT: Good donors in breeding for salt tolerance are a prerequisite for food security under changing climatic conditions. Horkuch, a farmer-popular salt tolerant rice (Oryza sativa L.) variety from the south-west coast of Bangladesh was characterised up to maturity under NaCl stress, together with a modern variety (BRRI dhan41), a sensitive control (BRRI dhan29) and Pokkali, the salt-tolerant benchmark for rice. Horkuch had low reduction in shoot biomass, a low Na :K ratio in !ag leaves, a low percent reduction in yield and good partitioning of Na in the older leaves, and maintained high levels of Ca and Mg in the !ag leaves. In order to understand the physiology at the molecular level, the expression of salt-responsive genes was investigated using microarray analysis. Salt-stressed cDNA of Horkuch seedlings were hybridised with cDNA probes synthesised mainly from database sequences of Arabidopsis thaliana (L.) Heynh. The upregulated genes included transcription factors, signal transducers, metabolic enzymes, reactive oxygen species (ROS) scavengers, osmoprotectants and some speci"c salt-induced transcripts. An increase in expression of photosynthesis-related genes as well ROS scavengers suggested that this could be the reason for the better yield performance of Horkuch. The data therefore indicate Horkuch as a potential donor alternative to Pokkali in breeding programs for salt tolerance.Functional plant Biology. 01/2011; 38:282-292. -
Article: Overexpression of a DEAD box helicase, PDH45, confers salinity tolerance to rice
[show abstract] [hide abstract]
ABSTRACT: To improve the salinity tolerance of rice, a DEAD-box helicase gene isolated from pea with a CaMV35S promoter was transformed into the Bangladeshi rice variety Binnatoa through Agrobacterium- mediated transformation. The transgenic seedlings showed significantly higher chlorophyll content, but decreased root length compared to wild type (WT) under normal physiological conditions. Their status was confirmed by polymerase chain reaction (PCR), semi-quantitative reverse-transcription PCR and Southern blot hybridization for positive integration of the transgene. The T2 progenies from three independent transformation events were characterized for salinity tolerance both at seedling and reproductive stages. Compared to the WT plants, the average decrease in chlorophyll content and dry weight of seedling leaves was lower by 20 and 12% respectively at 12 deciSiemens per meter (dS/m) NaCl stress in hydroponics. A higher leaf K?/Na? ratio of 0.346 was maintained by the transgenic lines compared to the WT ratio of 0.157, which indicated induced ion homeostasis. At the reproductive stage, transgenic rice plants expressing PDH45 showed better fertility and produced higher grain yield by 16% compared to WT plants under continuous stress of 6 dS/m from 30 days till maturity. One of the transformed lines, PDH45-P3, outperformed the others, and replicated data in reproductive stage soil stress of 12 dS/m NaCl showed its enhanced fertility and yield by 46 and 29% over WT, respectively.Molecular Breeding 01/2011; online. · 2.85 Impact Factor -
Article: Microsatellite marker diversity and sequence polymorphism in the red gene locus of indigenous rice populations of Bangladesh
[show abstract] [hide abstract]
ABSTRACT: Bangladesh is home to diverse rice germplasm, including red rice, many of which are preferred for cultivation by farmers over high-yielding rice varieties due to their special characteristics, color, taste, and nutritional value. Red color of seed pericarp is unusual among modern cultivated varieties, though it is a common characteristic in wild relatives of rice, making the trait an important parameter when studying the domestication and evolution of crop plants. Diversity analysis using microsatellite markers and sequence variation of the red rice loci of the indigenous rice population in Bangladesh was therefore performed. Microsatellite fingerprinting could successfully cluster cultivars according to their specific phenotypic characteristics such as stickiness or aroma, irrespective of their pericarp color, and locate a set of unique identifiers. Sequence analysis of a portion of the bHLH transcription factor gene Rc, which controls the red pigment, confirmed the occurrence of the 14-bp deletion in white rice accessions which has been reported previously. The analysis included a group of rice cultivars which are known by the same name but produce two different colored seeds and are indistinguishable morphologically unless dehusked. Statistical and internal transcribed spacer region sequence diversity analyses established that such cultivars, although genetically different, had very low diversity, suggesting a close evolutionary relationship between them. Red and white seeds from cultivars with the same name were planted individually over two generations and dehusked to check for loss/gain of pigmentation. Red-seeded plants produced some dirty-white seeds in addition to red ones in the first generation, the proportion of which increased when planted in the successive generation. This loss in pigment was probably due to faulty transcription, since no deletion in the respective region of the genome of the dirty-white seeds was noted.Plant Systematics and Evolution. 01/2011; 296:157-165. -
Article: Physiology and gene expression of the Rice (Oryza sativa L.) landrace Horkuch under salt stress
[show abstract] [hide abstract]
ABSTRACT: Good donors in breeding for salt tolerance are a prerequisite for food security under changing climatic conditions. Horkuch, a farmer-popular salt tolerant rice variety from the southwest coast of Bangladesh was characterized, together with a modern variety BRRI dhan41, a sensitive check BRRI dhan29, and Pokkali, the salt tolerant benchmark for rice, up to maturity under NaCl stress. Horkuch had low reduction in shoot biomass, low Na/K ratio in flag leaves, low percent reduction in yield, good partitioning of Na in the older leaves and maintained high levels of Ca and Mg in the flag leaves. In order to understand the physiology at the molecular level, the expression of salt responsive genes was investigated using microarray analysis. Salt stressed cDNA of Horkuch seedlings were hybridized with cDNA probes synthesized mainly from database sequences of A. thaliana. The upregulated genes included transcription factors, signal transducers, metabolic enzymes, reactive oxygen species scavengers, osmoprotectants as well as some specific salt-induced transcripts. Increase in expression of photosynthesis related genes as well ROS scavengers suggested that this could be the reason for the better yield performance of Horkuch. The data therefore indicate Horkuch as a potential donor alternative to Pokkali in breeding programs for salt tolerance.Functional Plant Biology. 01/2011;
