Rouhollah Nourmohammadi

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• Plasma thyroid hormone concentrations and pH values of some GI-tract segments of broilers fed on different dietary citric acid and microbial phytase levels

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Saraee, A. Arab

  American Journal of Animal and Veterinary Sciences. 6:1-6.

  Problem statement: An experiment was conducted to study the effect of microbial phytase supplementation and citric acid on thyroid activity, relative weight of lymphoid organs and pH values of someProblem statement: An experiment was conducted to study the effect of microbial phytase supplementation and citric acid on thyroid activity, relative weight of lymphoid organs and pH values of some GI-tract segments in broiler chickens fed corn-soybean meal based diets. Approach: The data was analyzed using a randomized complete block design (RCBD) with factorial arrangement of 3×3, three levels of citric acids (0, 3 and 6%) and three levels of phytase (0, 500 and 1000 IU kg-1). There were three replicates (with ten chicks in each replicate) for each treatment. A total of 270 Ross 308 broiler chicks were used. Results: Addition of citric acid to diets caused significant decrease in pH values of crop, gizzard, duodenum, jejunum and ileum (p<0.05) and caused significant increase (p<0.01) in plasma triiodothyronine (T3) concentration, T3:T4 ratio and relative weight of bursa and thymus, but had no significant effect on thyroxin (T4) concentrations. Microbial phytase significantly increased relative weight of thymus (p<0.01), but had no significant effects on thyroid gland activity, relative weight of bursa and values of pH in different parts of the GI-tract. Conclusion: Broiler chicks fed on acidifiers diets had better immune response resistance that lead to immunological advances. Also, decreasing pH in GI-tract by CA caused a beneficial effect in the inhibition of intestinal bacteria competition.

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• Effect of dietary citric acid and microbial phytase on broiler chicken performance and some carcass parameters

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Farhangfar

  AgEng 2010 Conference;

  A trial was conducted to determine the effects of dietary citric acid and microbial phytase on broiler chicken performance and some carcass parameters. The dietary treatments consisted of untreatedA trial was conducted to determine the effects of dietary citric acid and microbial phytase on broiler chicken performance and some carcass parameters. The dietary treatments consisted of untreated control feed. Treated feeds with the microbial enzyme phytase and citric acid were formulated using three levels of 0, 500 and 1000 U/Kg of microbial phytase and three levels of 0, 3 and %6 citric acid using 270 male Ross 308 broiler chickens were placed in an experimental design consisting of nine dietary treatments with three replicate cages of 10 broilers per cage. The dietary treatments consisted of untreated control feed, three microbial phytase-supplemented diets (0, 500 and 1000 U/kg), three citric acid feed (0, 3 and %6), 500 U/kg phytase + 3% citric acid, 1000 U/kg phytase + 3% citric acid, 500 U/kg phytase + 6% citric acid and 1000 U/kg phytase + 6% citric acid diets prepared on corn and soybean basis. The chickens were maintained on these dietary treatments from 7 to 42 d of age with feed and water made available for ad libitum consumption. Feed intake, body weight gain, final body weight, feed conversion ratio, live body weight, carcass weight and some relative internal organs weight were determined. There was a significant (P< 0.01) effect of citric acid diets in, feed intake, body weight gain and final body weight in the broilers fed the citric acid feeds but there was no significant effect in feed conversion ratio. Also, carcass weight was significantly affected by citric acid in the diet (P< 0.01) but relative internal organs weight was not significantly affected by citric acid of the diet. The microbial phytase treated diets significantly improved body weight and body weight gain (P< 0.05) but had no significance effect in feed intake and feed conversion ratio. At the same time, the microbial phytase diets significantly improved relative neck weight but relative carcass and related carcass factors were not significantly changed by affected by the microbial phytase diets. The results also indicated that there is an interaction between microbial phytase and citric acid and citric acid has a synergetic effect on phytase state of action in the body.

• Effect of citric acid and microbial phytase on growth performance and some carcass traits of broilers

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Farhangfar

  The fourth congress on animal science;

  A total of 270 Ross 308 male broilers from 7 to 42 days old were used in a 3×3 factorial arrangement design with three levels of citric acids (0, 3 and 6%) and three levels of phytase enzyme (0, 500A total of 270 Ross 308 male broilers from 7 to 42 days old were used in a 3×3 factorial arrangement design with three levels of citric acids (0, 3 and 6%) and three levels of phytase enzyme (0, 500 and 1000 enzyme IU/kg) on a randomized complete block design, with 9 experimental diets, 3 replicates and 10 birds per each replicate. The diets were formulated based upon corn-soybean meal. Feed intake (FI), weight gain (WG), feed conversion ratio (FCR), carcass yield and relative carcass weight factors were measured and analyzed throughout the experimental period. The diets containing citric acid had a significant effect (P<0.01) on feed intake, weight gain and carcass yield and had no significant effect on FCR and carcass factors. Weight gain and relative neck weight were significantly (P<0.05) affected by using microbial phytase enzyme, but had no significance effect on feed intake, feed conversion ratio and other carcass factors. Adding citric acid to diets containing phytase enzyme improved performance and carcass traits, therefore, the results showing that citric acid probably has a synergetic effect on phytase efficiency in broilers.

• Effect of citric acid and microbial phytase on serum enzyme activities and plasma minerals retention in broiler chicks

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Farhangfar

  South african journal of animal science.

• Effect of citric acid and microbial phytase supplementation on ileal digestibility of crude protein, apparent metabolizable energy, Ca and total P of broilers

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Farhangfar

  The fourth congress on animal science;

  An experiment was conducted to study the effect of microbial phytase supplementation and citric acid on ileal digestibility of some nutrients. A total of 270 Ross 308 male broilers were used in a 3×3An experiment was conducted to study the effect of microbial phytase supplementation and citric acid on ileal digestibility of some nutrients. A total of 270 Ross 308 male broilers were used in a 3×3 factorial arrangement design with three levels of citric acids (0, 3 and 6%) and three levels of phytase enzyme (0, 500 and 1000 enzyme IU/kg) on a randomized complete block design, with 9 experimental diets, 3 replicates and 10 birds per each replicate. The experimental diets were formulated based upon corn-soybean meal. Ileal digestibility of crude protein, apparent metabolizable energy, total P and Ca of broiler chicken were determined on day 35. The results showed that there was a significant (P<0.01) improvement effect of diet containing 3% citric acid on ileal digestibility of crude protein, apparent metabolizable energy and total P (P<0.01), but had no significant effect on digestibility of Ca. Also, diets having 6% citric acid significantly reduced ileal digestibility of crude protein, apparent metabolizable energy and total P (P<0.01) compared to diets having no acid or 3% citric acid (P<0.01). All different levels of microbial phytase improved nutrients digestibility of diets (P<0.01). The results also strongly showed that there is an interaction between citric acid and microbial phytase and acid has a synergetic effect on phytase efficiency in improvement of nutrients digestibility.

• Effect of Citric Acid and Microbial Phytase Supplementation on Ileal Protein Digestibility and Performance of Broilers

  Authors: Rouhollah Nourmohammadi

  Degree: M. Sc.

  Supervisor: Dr. Seyed Mohammad Hosseini

• Effect of dietary citric acid and microbial phytase on broiler chicken illume protein digestibility, metabolizable energy, CA and total P

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Farhangfar

  AgEng 2010 Conference;

  A trial was conducted to determine the effects of dietary citric acid and microbial phytase on broiler chicken ileal protein digestibility, metabolizable energy, Ca and total P of broiler chicken.A trial was conducted to determine the effects of dietary citric acid and microbial phytase on broiler chicken ileal protein digestibility, metabolizable energy, Ca and total P of broiler chicken. The dietary treatments consisted of untreated control feed. Treated feeds with the microbial enzyme phytase and citric acid were formulated using three levels of 0, 500 and 1000 U/Kg of microbial phytase and three levels of 0, 3 and %6 citric acid using 270 male Ross 308 broiler chickens were placed in an experimental design consisting of nine dietary treatments with three replicate cages of 10 broilers per cage. The dietary treatments consisted of untreated control feed, three microbial phytase-supplemented diets (0, 500 and 1000 U/kg), three citric acid feed (0, 3 and %6), 500 U/kg phytase + 3% citric acid, 1000 U/kg phytase + 3% citric acid, 500 U/kg phytase + 6% citric acid and 1000 U/kg phytase + 6% citric acid diets prepared on corn and soybean basis. The chickens were maintained on these dietary treatments from 7 to 42 d of age with feed and water made available for ad libitum consumption. Ileal protein digestibility, metabolizable energy, Ca and total P of broiler chicken on day 35 were determined. The result showed that there was a significant (P < 0.01) improvement effect of diet containing 3% citric acid in, protein digestibility, metabolizable energy and total P (P< 0.01) but had no significant effect in digestibility of Ca. Also, dietd having 6% citric acid significantly (P < 0.01) reduced, protein digestibility, metabolizable energy and total P (P< 0.01) compared to diets having no acid or 3% acid (P < 0.01). All different levels of microbial phytase improved nutrients digestibility of diets (P< 0.01). The results also strongly showed that there is an interaction between microbial phytase and citric acid and acid has a synergetic effect on phytase state of action in the body.

• Study on the stress effect of adding citric acid to broiler diet on production performance

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Farhangfar

  The first national conference of environmental stresses in agricultural science;

  270 Ross broiler chicks in a completely randomized design from 7-42 days old with 3 replication having 10 birds in each replicate, in a 3×3 factorial were used. The treatments included three levels270 Ross broiler chicks in a completely randomized design from 7-42 days old with 3 replication having 10 birds in each replicate, in a 3×3 factorial were used. The treatments included three levels of citric acids (0, 3 and %6) and three levels of phytase (0, 500 and 1000 enzyme unit). The results showed that addition of citric acid to diets having phytase significance effects (p<0.0001) on gaining weight and feed intake during the period of the experiment but had no significance effect on feed conversion ratio. Chicks having %6 citric acid in their diets had a significantly lower gaining weight and feed intake comparing to those having %3 citric acid in their diets (p<0.0001) but addition of %3 citric acid just numerically improved feed conversion ratio. Also interaction between citric acid and phytase was observed throughout the experimental period.

• Effect of Citric Acid and Microbial Phytase Enzyme on Ileal Digestibility of Some Nutrients in Broiler Chicks Fed Corn-Soybean Meal Diets

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Farhangfar

  Journal of Poultry Science.

• Effect of dietary acidification on some blood parameters and weekly performance of broiler chickens

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Farhangfar

  Journal of Animal and Veterinary Advances.

  A total number of 90 Ross 308 broiler chickens were randomly divided into three treatments with three replicates of 10 chicks each. The first treatment having 0% citric acid was served as control andA total number of 90 Ross 308 broiler chickens were randomly divided into three treatments with three replicates of 10 chicks each. The first treatment having 0% citric acid was served as control and fed corn-soybean meal base diet. While, the other two treatments were received the basal diet supplemented with citric acid at different levels of 3 and 6%, respectively. The experiment was lasted for 42 days. Some blood parameters (metabolites of serum, enzyme activities and plasma mineral concentrations), gastrointestinal tract (GI-tract) morphology and weekly performance were measured. The results indicated that diets including citric acid had significant effect (P<0.01) on weekly feed intake, body weight gain and body weight but there was no significant improvement on feed conversion ratio during any experimental weeks. Also, citric acid had significant effect on carcass yield (P<0.01) and relative heart weight (P<0.05) but had no significant effect on relative weights of liver and abdominal fat. The effect of citric acid was significant on GI-tract morphology, cholesterol concentration, serum alkaline phosphatase and lactate dehydrogenase activities (P<0.05) but did not significantly affect urea, triglycerides, total protein concentrations and serum alanine aminotransferase and aspartate aminotransferase activities. Dietary effect of citric acid was significantly shown on plasma P and Fe concentrations (P<0.05) but not shown on plasma Ca, Mg and Zn concentrations. The results showed that addition of 3% citric acid significantly improved weekly growth performance but adding 6% citric acid had a negative effect on weekly performance factors of broiler chicks.

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• Influence of citric acid and microbial phytase on growth performance and carcass characteristics of broiler chickens

  Authors: R. Nourmohammadi, S.M. Hosseini, H. Farhangfar

  American Journal of Animal and Veterinary Sciences. 5:282-288.

  Abstract: Problem statement: The aim of this study was to investigated the effects of adding citric acid and microbial phytase supplementation (Natuphos®) on growth performance and carcassAbstract: Problem statement: The aim of this study was to investigated the effects of adding citric acid and microbial phytase supplementation (Natuphos®) on growth performance and carcass characteristics of broiler chickens fed corn soybean meal base diets. Approach: The experiment included nine treatments with 10 birds in each replicate using a 3×3 factorial design for two main factors of citric acid (0, 3 and 6%) and three phytase enzyme (0, 500 and 1000 IU kg1). The diets were formulated based upon corn-soybean meal 7 to 21 and 22 to 42 day periods. Results: Using different levels of citric acid in diets had no effect on internal organs (except relative heart weight), whereas, diets containing 6% citric acid decreased feed intake, body weight gain and carcass yield (p<0.05) and improved feed conversion ratio and organs relative weight. Also, microbial phytase caused increase in feed intake, weight gain and relative neck weight (p<0.05). Conclusion: Depression of performance was differently affected by citric acid levels. Also, there was an additive effect between microbial phytase and citric acid.

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