Rodriguez-Mateos Ana

Publications (24) View all

• Article: Absorption, metabolism and vascular effects of alkalised cocoa

  M. J. Oruna-Concha, A. Rodriguez-Mateos, C. Kwik-Uribe, D. S. Mottram, G. R. Gibson, J. P. E. Spencer
  Proceedings of The Nutrition Society 01/2011; 70(OCE4-70):E224. · 2.77 Impact Factor
• Article: The citrus flavanone naringenin inhibits inflammatory signalling in glial cells and protects against neuroinflammatory injury.

  Katerina Vafeiadou, David Vauzour, Hung Yi Lee, Ana Rodriguez-Mateos, Robert J Williams, Jeremy P E Spencer
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  ABSTRACT: Neuroinflammation plays an integral role in the progression of neurodegeneration. In this study we investigated the anti-inflammatory effects of different classes of flavonoids (flavanones, flavanols and anthocyanidins) in primary mixed glial cells. We found that the flavanones naringenin and hesperetin and the flavanols (+)-catechin and (-)-epicatechin, but not the anthocyanidins cyanidin and pelargonidin, attenuated LPS/IFN-gamma-induced TNF-alpha production in glial cells. Naringenin also inhibited LPS/IFN-gamma-induced iNOS expression and nitric oxide production in glial cells, thus showing the strongest anti-inflammatory activity among all flavonoids tested. Moreover, naringenin protected against inflammatory-induced neuronal death in a primary neuronal-glial co-culture system. Naringenin also inhibited LPS/IFN-gamma-induced p38 mitogen-activated protein kinase (MAPK) phosphorylation and downstream signal transducer and activator of transcription-1 (STAT-1) in LPS/IFN-gamma stimulated primary mixed glial cells. Taken together, our results suggest that naringenin may produce an anti-inflammatory effect in LPS/IFN-gamma stimulated glial cells that may be due to its interaction with p38 signalling cascades and the STAT-1 transcription factor.
  Archives of Biochemistry and Biophysics 05/2009; 484(1):100-9. · 2.93 Impact Factor
• Article: The RISCK study: impact of the amount and type of dietary fat and carbohydrate on the plasma phospholipid-fatty acid status of free-living individuals

  Julie Lovegrove, Ana Rodriguez Mateos, Gary Frost, Bruce Griffin, Tom Sanders, Carmel Moore
  Proceedings of The Nutrition Society 01/2009; 67(OCE8):E312. · 2.77 Impact Factor
• Article: Daily consumption of an aqueous green tea extract supplement does not impair liver function or alter cardiovascular disease risk biomarkers in healthy men.

  Jan Frank, Trevor W George, John K Lodge, Ana M Rodriguez-Mateos, Jeremy P E Spencer, Anne Marie Minihane, Gerald Rimbach
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  ABSTRACT: Regular consumption of green tea polyphenols (GTP) is thought to reduce the risk of cardiovascular disease (CVD) but has also been associated with liver toxicity. The present trial aimed to assess the safety and potential CVD health beneficial effects of daily GTP consumption. We conducted a placebo-controlled parallel study to evaluate the chronic effects of GTP on liver function and CVD risk biomarkers in healthy men. Volunteers (treatment: n = 17, BMI 26.7 +/- 3.3 kg/m(2), age 41 +/- 9 y; placebo, n = 16, BMI 25.4 +/- 3.3 kg/m(2), age 40 +/- 10 y) consumed for 3 wk 6 capsules per day (2 before each principal meal) containing green tea extracts (equivalent to 714 mg/d GTP) or placebo. At the beginning and end of the intervention period, we collected blood samples from fasting subjects and measured vascular tone using Laser Doppler Iontophoresis. Biomarkers of liver function and CVD risk (including blood pressure, plasma lipids, and asymmetric dimethylarginine) were unaffected by GTP consumption. After treatment, the ratio of total:HDL cholesterol was significantly reduced in participants taking GTP capsules compared with baseline. Endothelial-dependent and -independent vascular reactivity did not significantly differ between treatments. In conclusion, the present data suggests that the daily consumption of high doses of GTP by healthy men for 3 wk is safe but without effects on CVD risk biomarkers other than the total:HDL cholesterol ratio.
  Journal of Nutrition 01/2009; 139(1):58-62. · 3.92 Impact Factor
• Source

  Available from: Miriam M. Cortese-Krott

  Article: A multilevel analytical approach for detection and visualization of intracellular NO production and nitrosation events using diaminofluoresceins.

  Miriam M Cortese-Krott, Ana Rodriguez-Mateos, Gunter Kuhnle, Geoff Brown, Martin Feelisch, Malte Kelm
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  ABSTRACT: Diaminofluoresceins are widely used probes for detection and intracellular localization of NO formation in cultured/isolated cells and intact tissues. The fluorinated derivative 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM) has gained increasing popularity in recent years because of its improved NO sensitivity, pH stability, and resistance to photobleaching compared to the first-generation compound, DAF-2. Detection of NO production by either reagent relies on conversion of the parent compound into a fluorescent triazole, DAF-FM-T and DAF-2-T, respectively. Although this reaction is specific for NO and/or reactive nitrosating species, it is also affected by the presence of oxidants/antioxidants. Moreover, the reaction with other molecules can lead to the formation of fluorescent products other than the expected triazole. Thus additional controls and structural confirmation of the reaction products are essential. Using human red blood cells as an exemplary cellular system we here describe robust protocols for the analysis of intracellular DAF-FM-T formation using an array of fluorescence-based methods (laser-scanning fluorescence microscopy, flow cytometry, and fluorimetry) and analytical separation techniques (reversed-phase HPLC and LC-MS/MS). When used in combination, these assays afford unequivocal identification of the fluorescent signal as being derived from NO and are applicable to most other cellular systems without or with only minor modifications.
  Free radical biology & medicine 09/2012; · 5.42 Impact Factor