Research experience
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May 2008
Research: Uranium uptake studies
NFMC · Atomic energy · NFMCNFMC
Publications (1) View all
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Article: Journal : Bioresource Technology Siderophore mediated uranium sequestration by marine cyanobacterium Synechococcus elongatus BDU 130911 Vijayaraghavan Rashmi, Mohandass ShylajaNaciyar, Ramamoorthy Rajalakshmi, Stanley.F.D’ Souza, Dharmar Prabaharan, Lakshmanan Uma
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ABSTRACT: Four different marine cyanobacterial morphotypes were tested for their efficacy to produce siderophores in Fe minus [Fe(-)], Fe minus Uranium dosed [(Fe(-)U(+)], and Fe dosed Uranium dosed [(Fe(+)U(+)] media. Of the four organisms tested, Synechococcus elongatus BDU 130911 produced the highest amount of siderophore of 58μgmg-1dryweight. The results clearly indicate that uranium induces siderophore production in marine cyanobacteria even in the presence of iron [(Fe(+)U(+)] condition. The type of siderophore revealed by FeCl3, Tetrazolium and Atkin’s tests is a hydroxamate; and thin layer chromatogram also authenticates our finding. Uranium siderophore complexation was confirmed through modified Chrome Azurol S (CAS) assay as well as based on residual uranium presence. In silico docking studies further validate siderophore complexation with uranium.Bioresource Technology 12/2012; · 4.98 Impact Factor
