Publications (3) View all
-
Article: Performance of the GenoType(R) MTBDRplus assay directly on sputum specimens from Brazilian patients with tuberculosis treatment failure or relapse.
Raquel de Abreu Maschmann, Fernanda Sá Spies, Luciana de Souza Nunes, Andrezza Woloski Ribeiro, Taís Raquel Marcon Machado, Arnaldo Zaha, Maria Lucia Rosa Rossetti[show abstract] [hide abstract]
ABSTRACT: Rapid identification of drug resistance in clinical isolates of Mycobacterium tuberculosis is important to adequate treatment of tuberculosis. The aim of this work was evaluate the performance of GenoType® MDRTBplus directly from sputum of patients who had treatment failure or relapse in an outpatient routine setting in southern Brazil.Journal of clinical microbiology 03/2013; · 4.16 Impact Factor -
SourceAvailable from: Rosa Dea Sperhacke
Article: Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay.
Raquel de A Maschmann, Mirela Verza, Marcia Sn Silva, Rosa Dea Sperhacke, Marta O Ribeiro, Philip Noel Suffys, Harrison Magdinier Gomes, Enrico Tortoli, Fiorella Marcelli, Arnaldo Zaha, Maria Lucia R Rossetti[show abstract] [hide abstract]
ABSTRACT: We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were 92.3% and 98.1%, respectively. When compared with sequencing, the sensitivity and specificity of the CRDH were 90.6% and 100%, respectively. To evaluate the performance of the assay directly in clinical specimens, 30 samples from tuberculosis patients were used. For these samples, the results of the CRDH were 100% consistent with the results of the AST and sequencing. These results indicate that the rate of concordance of the CRDH is high when compared to conventional methods and sequencing data. The CRDH can be successfully applied when a rapid test is required for the identification of RIF resistance in M. tuberculosis.Memórias do Instituto Oswaldo Cruz 03/2011; 106(2):139-45. · 2.15 Impact Factor -
Article: In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis.
Mirela Verza, Raquel de Abreu Maschmann, Márcia Susana Nunes Silva, Elis Regina Dalla Costa, Marta Osório Ribeiro, Franciele Rosso, Philip Noel Suffys, Enrico Tortoli, Fiorella Marcelli, Arnaldo Zaha, Maria Lucia Rosa Rossetti[show abstract] [hide abstract]
ABSTRACT: Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC-->ACC (Ser-->Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.Memórias do Instituto Oswaldo Cruz 08/2009; 104(5):710-4. · 2.15 Impact Factor