Publications (2) View all
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Article: In vivo and in vitro anti-inflammatory potential of pentahydroxy-pregn-14-ol, 20-one-β-d-thevetopyranoside in rats.
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ABSTRACT: Polyhydroxy pregnane glycoside (PPG), a steroidal glycoside was isolated from Wattakaka volubilis Linn. (Stap.f.). PPG was evaluated for in vivo and in vitro anti-inflammatory activity using acute inflammation and chronic model of inflammation in rats and LPS-induced RAW 264.7 macrophage cells. PPG seemed to be responsible for the anti-inflammatory activity in the studied models. PPG at dose level of both 5 and 10mg/kg significantly reduced the edema induced by the carrageenan in acute model of inflammation. It also showed significant anti-proliferative effect (dry pellet weight basis) in chronic model of inflammation. Cellular content of granuloma was measured by assaying activity of N-acetyl glucosaminidase (NAG) and total nucleic acid content. PPG at 5 and 10mg/kg significantly suppressed the cellular infiltration measured by total nucleic acid content. In contrast, NAG activity decreased over a period of 10 days resulting in inhibition of granuloma weight gain. PPG had a more effective response than the reference drug diclofenac sodium in both the models of inflammation. Wattakaka volubilis steroidal glycoside mixture (WVSM) and PPG (1-50μM) significantly inhibited the COX-2 and iNOS enzymes resulting in low levels of PGE2 and NO in LPS-induced RAW 264.7 macrophage cells. Hence the study supports the traditional use of Wattakaka volubilis and its constituent PPG in treatment of inflammatory disorders.Phytomedicine: international journal of phytotherapy and phytopharmacology 04/2013; · 2.17 Impact Factor -
Article: Neuroprotective effects of polyhydroxy pregnane glycosides isolated from Wattakaka volubilis (L.f.) Stapf. after middle cerebral artery occlusion and reperfusion in rats.
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ABSTRACT: Wattakaka volubilis (L.f) (Stapf.) is well known for its rich steroid pregnane contents and this plant is known for its potential use as alternative biological activities. However, little is known about its neuroprotective effect in ischemia-reperfusion (I/R)-induced cerebral injury. Hence, the present study was carried out to investigate the effect of Wattakaka volubilis(L.f) (Stapf.) sapogenin glycosides mixture (WVSM) and polyhydroxy pregnane glycoside (PPG) isolated from it on neuroprotection and the potential mechanisms in the rat I/R model induced by middle cerebral artery occlusion (MCAO). The rats were pretreated with WVSM (50mg/kg) and PPG (5 and 10mg/kg) (p.o.) once a day for 5 days before MCAO. Neurological deficit score (NDS), rotarod performance, brain water content, 2,3,5-triphenyltetrazolium chloride (TTC) staining and indices of oxidative stress [superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO) and nitric oxide synthase (NOS)] were measured at 24h after ischemia. The results indicated that pretreatment with WVSM and PPG (10mg/kg) significantly diminished the volume of infarction and brain water content, and improved NDS. Moreover, WVSM and PPG (10mg/kg) markedly reversed the level of MDA, NO, NOS and SOD to their normal state in serum or cerebral ischemic section. These findings demonstrate that WVSM and PPG (10mg/kg) exhibit neuroprotective effect through its anti-oxidative action in MCAO rats.Brain research 04/2013; · 2.46 Impact Factor
