R. S. Chauhan

Ph. D
TRP & OIC Medicinal and Aromatic Plants
Uttarakhand University of Hort... · Medicinal & Aromatic Plants

Publications

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    Dataset: 3
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    ABSTRACT: Dactylorhiza hatagirea (D. Don) Soo (Orchidaceae) is an important endangered medicinal herb, distributed in subalpine to alpine regions of the Himalayas. Its tubers are important constituents of many medicines and health tonics. Overexploitation for medicinal uses has decreased availability in natural habitats and this species has been enlisted as endangered, making conservation and cultivation studies necessary. Variability studies may serve as an important tool for effective conservation and for a crop improvement program. Therefore, natural populations of D. hatagirea were analyzed for variability on the basis of morphological, biochemical, and isoenzyme patterns. The studied populations were grouped into two clusters. Existing variability among different populations opens up new areas for conservation and perspectives for a genetic improvement program for D. hatagirea.
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    R.S. Chauhan, M.C. Nautiyal, A. Tava, R. Cecotti
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    ABSTRACT: Heracleum candicans Wall. (Apiaceae) is an important endangered aromatic species, exploited commercially with a destructive harvesting system from its natural habitat. We cultivated a wild strain of H. candicans, harvested leaves for extraction of essential oil and analyzed them. Twenty-eight compounds were identified from essential oil using gas chromatography–flame ionization detection (GC–FID) and GC–mass spectrometry (GC–MS) analysis. These compounds are very useful for the pharmaceutical, flavor and fragrance industries. Harvesting of the leaves for the production of essential oil is a sustainable method compared with uprooting plants. Being a perennial species, leaves can be harvested every year, as the aerial parts of the plant regenerate easily and farmers need not worry about planting every year.
    Journal of Essential Oil Research 01/2014; 26(2). · 0.55 Impact Factor
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    ABSTRACT: Dactylorhiza hatagirea (D. Don) Soo (Orchidaceae) is an important endangered medicinal herb, distributed in subalpine to alpine regions of the Himalayas. Its tubers are important constituents of many medicines and health tonics. Overexploitation for medicinal uses has decreased availability in natural habitats and this species has been enlisted as endangered, making conservation and cultivation studies necessary. Variability studies may serve as an important tool for effective conservation and for a crop improvement program. Therefore, natural populations of D. hatagirea were analyzed for variability on the basis of morphological, biochemical, and isoenzyme patterns. The studied populations were grouped into two clusters. Existing variability among different populations opens up new areas for conservation and perspectives for a genetic improvement program for D. hatagirea.
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    R.S. Chauhan, M.C. Nautiyal, Aldo Tava
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    ABSTRACT: Uttarakhand has been declared ‘Herbal State’, but herbal wealth of this state could not be utilized properly due to lack of chemical characterization of most of the vegetational wealth. Mentha spicata L. (spearmint) was collected from naturally growing population of Uttarakhand (3800 ft). GC-MS analysis reveals details of 94.0 % of essential oil. Carvone was major part (57.1 %) whereas other components were limonene (10.1 %), cis-dihydrocarvone (3.2 %), dihydrocarveol (3.2 %).
    Journal of essential oil-bearing plants JEOP 03/2013; 13(3):353-356. · 0.29 Impact Factor
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    V. K. Purohit, Y.M. Bahuguna, D. Tewari, A. Tewari, H. C. Andola, J. S. Negi and R.S. Chauhan
    Current science 01/2013; 104(2):161-162. · 0.91 Impact Factor
  • Chemistry of Natural Compounds 01/2012; 48,(5):901-902. · 0.60 Impact Factor
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    Current science 01/2012; 103 (3), 251-252. · 0.91 Impact Factor
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    ABSTRACT: The essential oil from the underground parts of Selinum tenuifoliumWall. (Apiaceae) was extracted by hydrodistillation using a Clevenger-type apparatus, and analyzed by GC/FID and GC/MS. Nine constituents, representing 97.7% of the total oil, were identified, five of which belong to the class of polyacetylenes. The structures of the compounds 1 – 5 were elucidated by using IR, MS, and 1H- and 13C-NMR data after purification by column chromatography. The major constituent detected was nona- 3,5-diyne (1; 85.6% of the total volatiles), followed by nona-3,5-diyn-2-one (2), nona-4,6-diyn-3-one (3), nona-3,5-diyn-2-ol (4), and nona-4,6-diyn-3-ol (5), accounting for 3.0, 2.5, 2.2, and 3.1% of the total volatiles, respectively. The latter four polyacetylenes, 2 – 5, were never reported in plants so far, and, therefore should be regarded as novel compounds.
    Helvetica Chimica Acta 01/2012; 95:780-787. · 1.38 Impact Factor
  • R.S. Chauhan and Prabhu Dutt
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    ABSTRACT: Swertia anguistifolia Ham. is an important medicinal plant of family Gentianaceae, distributed in subtropical to temperate Himalaya. The species is adulterant of S. chirayita and useful in ethnomedicinal system. Mature plants were collected at flowering stage, air dried, crushed to make extracts. Samples were investigated for the presence of biologically active molecules. Samples of S. anguistifolia collected from four different populations’ revealed presence of mangiferin and amaroswerin whereas amarogentin were completely absent. Concentration of mangiferin and amaroswerin varied from population to population.
    Phytomedicine 01/2012; 4 (2), 94-97:94-97. · 2.97 Impact Factor
  • R.S. Chauhan and Prabhu Dutt
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    ABSTRACT: Medicinal properties of mangiferin, amaroswerin and amarogentin from genus Swertia are well documented. Collection of S. chirayita is strictly prohibited due to critically endangered status. Therefore, new source of these medicinal compounds is necessary for regular supply in market to combat various diseases. To identify new source of mangiferin, amaroswerin and amarogentin several plant species were screened. S. alternifolia showed presence of C-glucoxanthone mangiferin along with bitter secoiridoid glucosides (amaroswerin and amarogentin). As S. chirayita had been banned for collection due to rarity in nature, S. alternifolia can be used as an alternate source of mangiferin, amaroswerin and amarogentin for medicinal use.
    Journal of Natural Products 01/2012; 2, 104-109:104-109. · 3.29 Impact Factor
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    ABSTRACT: Morina longifolia Wall. ex DC. is an important aromatic plant that is also well-known in ethnomedicine in the Himalayan region. The aerial parts of the plant were collected during the flowering stage and steam distilled in order to characterize the essential oil composition. Thirty-three compounds constituting 94.2% of the total volatiles were identified using gas chromatography–flame ionization detection (GC–FID) and GC–mass spectrometry (GC–MS). The major constituent of the volatile fraction was β-myrcene (42.5%), whereas other important constituents were bicyclogermacrene (8.9%), germacrene D (6.7%) and limonene (6.3%). The characterization of the essential oil might help out to establish new potential applications for this species, both as herbal supplement and as fragrance agent.
    Journal of Essential Oil Research 01/2012; 24 (5), 461-463(5):461-463. · 0.55 Impact Factor
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    ABSTRACT: Selinum candollii DC. (Apiaceae) is an important perennial medicinal and aromatic plant species (MAPs) grows in humus rich mountain slopes. Underground parts were collected from Tungnath (12,000 ft asl), extracted for essential oil and analyzed by GC-FID and GC-MS. Chemical composition of essential oil described with possible uses of constituents for food, flavor and pharmaceutical sector.
    Journal of essential oil-bearing plants JEOP 01/2012; 15(6):864-867. · 0.29 Impact Factor
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    Journal of Plant Breeding and Crop Science. 12/2011; 3(9):190-194.
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    ABSTRACT: Nardostachys jatamansi DC. is an endangered medicinal and aromatic plant species. Considering the status and importance, there is an urgent need of its conservation, cultivation and crop improvement. Variability studies may serve as an important tool for effective conservation and crop improvement program. Therefore, germplasm was collected from different natural population and transplanted in Tungnath. Different morphometric traits along with essential oil content (%) were used for variability analysis. Cluster 3 (accession NJ5 and NJ6) showed best performance among most of the studied characters. Therefore, germplasm should be collected from Tungnath and the Valley of flowers population for large scale cultivation.
    International Journal of Plant Breeding and Genetics 01/2011; 3 (9), 190-194.
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    ABSTRACT: Hypericum perforatum L. (Hypericaceae) is the most commercially important species within the genus Hypericum. A wild strain was cultivated at Pothiwasa (2200 m), Uttarakhand, India. Aerial parts were collected (the upper two-thirds) during the flowering phenophase and used to extract the essential oil by means of a Clevenger-type apparatus. Forty compounds constituting 91.0% of the total volatile oil were identified using GC-FID and GC/MS analysis. The major constituent of the essential oil was germacrene D (22.1%), whereas other important constituents were found to be β-caryophyllene (11.3%), α-pinene (8.6%), α-cadinol (4.4%), β-pinene (3.8%), 2-methyl-octane (3.7%), terpinen-4-ol (3.3%), caryophyllene oxide (3.3%), α-muurolol (2.9%) and spathulenol (2.8%). The chemical composition of the oil varied qualitatively and quantitatively as compared to previous investigations. The peculiarity of the oil composition from the sample investigated in this paper may be attributed to environmental factors, such as soil nutrient status and growth environment, as well as to the genetic features of the cultivated strain
    Journal of Essential Oil Research 01/2011; 23(3):20-25. · 0.55 Impact Factor
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    ABSTRACT: Artemisia dracunculus L. (Tarragon) is an important species of Asteraceae. Plants were collected at flowering stage from naturally growing population of Shansha (Himachal Pradesh), North-West Himalaya, India. Air dried shoots (room temperature 25–35 °C) were used to extract essential oil using Clevenger-type apparatus for 3 h and analyzed. Twenty-four compounds were identified using GC-FID and GC–MS analysis. Major constituent of essential oil was capillene (58.38%), whereas other constituents were Z-β-ocimene (8.63%), β-phellandrene (7.03%), terpinenolene (5.87%), camphene (4.16%), spathulenol (2.02%), β-pinene (1.02%), etc. Chemical composition of essential oil is described and compared with earlier studies. The population is categorized as chemotype of A. dracunculus. As capillene is major constituents, this chemotype may be useful for industrial exploitation as well as chemotaxonomic characterization.
    Industrial Crops and Products. 05/2010;
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    ABSTRACT: Artemisia dracunculus L. (Tarragon) is an important species of Asteraceae. Plants were collected at flowering stage from naturally growing population of Shansha (Himachal Pradesh), North-West Himalaya, India. Air dried shoots (room temperature 25–35 ◦C) were used to extract essential oil using Clevengertype apparatus for 3 h and analyzed. Twenty-four compounds were identified using GC-FID and GC–MS analysis. Major constituent of essential oil was capillene (58.38%), whereas other constituents were Z-�- ocimene (8.63%), �-phellandrene (7.03%), terpinenolene (5.87%), camphene (4.16%), spathulenol (2.02%), �-pinene (1.02%), etc. Chemical composition of essential oil is described and compared with earlier studies. The population is categorized as chemotype of A. dracunculus. As capillene is major constituents, this chemotype may be useful for industrial exploitation as well as chemotaxonomic characterization.
    Industrial Crops and Products 01/2010; 31 (2010)(31 (2010) 546–549):546–549. · 2.47 Impact Factor
  • R. S.Chauhan
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    ABSTRACT: Traditional cereal crop production is uneconomic, and diversification in cropping system is necessary in mountain regions. A pilot project was introduced on medicinal and aromatic plants (MAPs) cultivation with semi-processing and market strategy. Fast growing MAPs with high economic potential were given priority. A cooperative society was organized for quality control and proper marketing. Farmers got additional income with sale of MAPs that could improve socioeconomy. In the depressing scenario of the deteriorating farming condition, emerging experience of MAPs farming give hope for better living standard of mountain peoples. Cultivation of economically viable MAPs can improve socioeconomy of farmers.
    Journal of Sustainable Agriculture 01/2010; 34:647–658. · 0.74 Impact Factor

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