Publications (2) View all
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Article: RP-HPLC Method Development and Validation for the Simultaneous Estimation of Satranidazole and Ofloxacin in Pharmaceutical Dosage Form.
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ABSTRACT: rapid, and accurate reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous determination of ofloxacin (OFL) and satranidazole (SAT) in combination. The separation is carried out using a mobile phase consisting of 10mM phosphate buffer and methanol in the ratio of 50:50. The pH of the mobile phase is adjusted to 3.0 with 10% o-phosphoric acid. The column used is Kromasil-100 C 18 (250 × 4.6 mm, 5 µm). with flow rate of 1.0 mL/min using UV detection at 294nm. The total run time is 5 min and the retention time of OFL and SAT is 2.59 min and 4.0 min respectively. The described method is linear for the assay of OFL and SAT over a concentration range of 10–24 µg/mL and 15–36 µg/mL respectively. Results of the analysis have been validated statistically and by recovery studies. The limit of quantitation for SAT and OFL has been found to be 0.042 µg/mL and 0.085 µg/mL respectively. The results of the studies showed that the proposed RP-HPLC method is simple, rapid, precise, and accurate, which is useful for the routine determination of SAT and OFL in bulk drug and its pharmaceutical dosage form.Journal of chromatographic science 11/2009; 49. · 0.88 Impact Factor -
Article: STEADY-STATE PHARMACOKINETICS OF IMMEDIATE-RELEASE AND CONTROLLED-RELEASE METRONIDAZOLE TABLETS
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ABSTRACT: The objective of the study was to evaluate the plasma levels of metronidazole in Controlled Release (CR) tablets given once daily and comparing it with Immediate Release (IR) metronidazole tablets given thrice daily, in twelve healthy adult male human subjects, dosed under fasting conditions for 5 days till a steady state was reached. On the 6 th A high-performance liquid chromatographic (HPLC) method for the pharmacokinetic analysis of metronidazole was developed to quantify metronidazole in plasma samples. Extraction of analyte and Internal Standard (Tinidazole) from plasma involved protein precipitation with 20% trichloroacetic acid solution in methanol. Chromatographic separation was achieved with Kromasil-100 C day, after dosing, the elimination of the drug was studied. 18 (250 × 4.6 mm) 5 µm column and 50 mM KH2PO4 with 0.1% Triethylamine (pH 6.0 with dil. H3PO4): acetonitrile (85:15, v/v) as the mobile phase with flow rate 1ml/min and UV detection at 320 nm. The calibration curve was linear in the concentration range 2.0 – 80.0 µg/ml. When the pharmacokinetic parameters of metronidazole in the two formulations were calculated and compared statistically using analysis of variance (ANOVA), they were similar, without any statistically significant difference. Pharmacokinetic parameters including AUC (0-t) , AUC (0-∞) , Cmax, Tmax, T1/2 and KelInternational Journal of Pharmacy and Pharmaceutical Sciences 04/2012;
