Peerasak Srinives

Publications (46) View all

• Article: Quantitative trait locus mapping reveals conservation of major and minor loci for powdery mildew resistance in four sources of resistance in mungbean [Vigna radiata (L.) Wilczek]

  Sompong Chankaew, Prakit Somta, Takehisa Isemura, Norihiko Tomooka, Akito Kaga, Duncan A. Vaughan, Peerasak Srinives
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  ABSTRACT: Powdery mildew (PM) is a common and serious disease of mungbean [Vigna radiata (L.) Wilczek]. A few quantitative trait loci (QTL) for PM resistance in mungbean have been reported. The objective of this study was to locate QTL for PM resistance in two resistant accessions V4718 and RUM5. Simple sequence repeat markers were analyzed in an F2 population from a cross between Kamphaeng Saen 1 (KPS1; susceptible to PM) and V4718 (resistant to PM), and in F2 and BC1F1 populations from a cross between Chai Nat 60 (CN60; susceptible to PM) and RUM5 (resistant to PM). Progenies of 134 F2:3 and F2:4 lines derived from KPS1 × V4718, and 190 F2:3 and 74 BC1F1:2 lines derived from CN60 × RUM5 and CN60 × (CN60 × RUM5), respectively, were evaluated for response to PM under field conditions. Multiple interval mapping identified a major QTL on linkage group (LG) 9 and two minor QTL on LG4 for the resistance in V4718, and detected two major QTL on LG6 and LG9 and one minor QTL on LG4 for the resistance in RUM5. Comparative linkage analysis of the QTL for PM resistance in this study and in previous reports suggests that the resistance QTL on LG9 in V4718, RUM5, ATF3640 and VC6468-11-1A are the same locus or linked. One QTL on LG4 is the same in three sources (V4718, RUM5 and VC1210A). Another QTL on LG6 is the same in two sources (RUM5 and VC6468-11-1A). In addition, one QTL in V4718 on LG4 appears to be a new resistance locus. These different resistance loci will be useful for breeding durably PM-resistant mungbean cultivars.
  Molecular Breeding 04/2013; · 2.85 Impact Factor
• Source

  Available from: Prakit Somta

  Article: A single recessive gene controls fragrance in cucumber (Cucumis sativus L.)

  Pornkamon Pramnoi, Prakit Somta, Sompong Chankaew, Ruangchai Juwattanasomran, Peerasak Srinives
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  ABSTRACT: None
  Journal of Genetics 04/2013; 92(1):147-149. · 1.09 Impact Factor
• Source

  Available from: Tanapon Chaisan

  Article: Development of Tetraploid Plants from an Interspecific Hybrid between Mungbean (Vigna radiata) and Rice Bean (Vigna umbellata)

  Tanapon Chaisan, Prakit Somta, Peerasak Srinives, Sontichai Chanprame, Rangsarid Kaveeta, Surapong Dumrongkittikule
  Journa of Crop Science and Biotechnology. 03/2013;
• Article: Mapping QTL conferring resistance to iron deficiency chlorosis in mungbean [Vigna radiata (L.) Wilczek]

  Prayoon Prathet, Prakit Somta, Peerasak Srinives
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  ABSTRACT: Foliar chlorosis caused by iron deficiency of plants grown on calcareous soil results in substantial crop yield loss and is an important in crop production problem. The objective of this study was to identify the quantitative trait locus (QTL) controlling resistance to iron deficiency chlorosis (IDC) in mungbean. An RIL population of 122 F8 lines developed from the cross between a susceptible cultivar, “Kamphaeng Saen 2” and a resistant line, “NM10-12” was genetically analyzed with simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. The population was evaluated for IDC resistance in an iron deficient field by visually scoring and SPAD measurements in 2010 and 2011. Segregation of the visual scores and SPAD values of the RILs in both years suggested quantitative inheritance of the resistance to IDC. Visual score and SPAD value from each year and combined data were used for QTL analysis. Single marker analysis revealed that 12 DNA markers from 3 linkage groups (LG) associated with the resistance. Four SSR and two AFLP markers on LG 3 associated with the resistance in all cases. Composite interval mapping identified two QTLs, qIDC3.1 and qIDC2.1, controlling the resistance. qIDC3.1 on LG 3 was identified from visual scores and SPAD values to account for 12.12% and 41.67% of the total variation depending on traits measured and years. qIDC2.1 on LG 2 was detected only from visual score data in 2010 and explained 45.66% of the total variation. The qIDC3.1 was the same as qIR which was the major QTL previously reported for IDC in mungbean grown in hydroponic conditions. The SSR markers CEDG084 and CEDC031 flanked and closely linked to the qIDC are useful for marker-assisted selection for mungbean resistance to IDC.
  Field Crops Research 10/2012; 137(1):230-236. · 2.47 Impact Factor
• Source

  Available from: Alisa Kongjaimun

  Article: QTL mapping of pod tenderness and total soluble solid in yardlong bean [Vigna unguiculata (L.) Walp. subsp. unguiculata cv.-gr. sesquipedalis]

  Alisa Kongjaimun, bullet Prakit, Somta @bullet, Norihiko Tomooka, bullet Akito, Kaga @bullet, Duncan A Vaughan, Peerasak Srinives
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  ABSTRACT: Yardlong bean [Vigna unguiculata ssp. unguiculata cv.-gr. sesquipedalis] is an important vegetable legume, particularly in Asia. Tenderness and sweetness of fresh pods are the key factors in deciding the commercial acceptance of yardlong bean. We report here for the first time quantitative trait loci (QTL) mapping of these traits from crosses between the yardlong bean accession JP81610 and wild cowpea (V. unguiculata ssp. unguiculata var. spontanea) acces-sion JP89083. Two SSR-based linkage maps developed from BC 1 F 1 [(JP81610 9 JP89083) 9 JP81610] and F 2 (JP81610 9 JP89083) populations were used for QTL analysis of pod tenderness and total soluble solid (TSS) content. Composite interval mapping (CIM) identified three QTLs for pod tenderness with pheno-typic variance explained (PVE) of 5.6–50 % and alleles from JP81610 increased the tenderness. CIM detected two QTLs for pod TSS with PVE of 7 and 9 %, and alleles from JP89083 increased TSS. Locations of these QTLs were compared with those of QTLs controlling domestication-related traits identified in the same populations. All QTLs for pod tenderness co-localized with QTLs for pod length. QTLs for pod TSS co-located with QTLs for pod dehiscence and/or pod length. The implications of these QTLs in breeding new yardlong bean and cowpea cultivars are discussed.
  Euphytica 08/2012; 189:217-223. · 1.55 Impact Factor