Ouattara Lassina

Publications (7) View all

• Article: Screening Togolese medicinal plants for few pharmacological properties.

  Simplice D Karou, Tchadjobo Tchacondo, Micheline Agassounon Djikpo Tchibozo, Kokou Anani, Lassina Ouattara, Jacques Simpore, Comlan de Souza
  [show abstract] [hide abstract]
  ABSTRACT: Terminalia macroptera Guill. et Perr. (Combretaceae), Sida alba L. (Malvaceae), Prosopis africana Guill et Perr. Taub. (Mimosaceae), Bridelia ferruginea Benth. (Euphorbiaceae), and Vetiveria nigritana Stapf. (Asteraceae) are traditionally used in Togolese folk medicine to treat several diseases including microbial infections. This study aimed to investigate the antimicrobial, antioxidant, and hemolytic properties of the crude extracts of the above-mentioned plants. The antimicrobial and the antioxidant activities were assayed using the NCCLS microdilution method and the DPPH free radical scavenging, respectively. Human A+ red blood cells were used to perform the hemolytic assay. Phenolics were further quantified in the extracts using spectrophotometric methods. Minimal inhibitory concentrations in the range of 230-1800 μg/ml were recorded in the NCCLS broth microdilution for both bacterial and fungal strains with methanol extracts. The DPPH radical scavenging assay yielded interesting antioxidant activities of the extracts of P. africana and T. macroptera (IC(50) values of 0.003 ± 0.00 μg/ml and 0.05 ± 0.03 μg/ml, respectively). These activities were positively correlated with the total phenolic contents and negatively correlated with the proanthocyanidin content of the extracts. The hemolytic assay revealed that great hemolysis occurred with the methanol extracts of T. macroptera, S. longepedunculata, and B. ferruginea. These results support in part the use of the selected plants in the treatment of microbial infections. In addition, the plant showed an interesting antioxidant activity that could be useful in the management of oxidative stress.
  Pharmacognosy Research 04/2012; 4(2):116-22.
• Article: Antimicrobial, antiplasmodial, haemolytic and antioxidant activities of crude extracts from three selected Togolese medicinal plants.

  Simplice D Karou, Tchadjobo Tchacondo, Lassina Ouattara, Kokou Anani, Aly Savadogo, Amegnona Agbonon, Mossaclok Ben Attaia, Comlan de Souza, Mohsen Sakly, Jacques Simpore
  [show abstract] [hide abstract]
  ABSTRACT: To investigate the antioxidant, antimicrobial, antiplasmodial, acute toxicity and haemolytic activities of methanolic extracts of three plants. Phytochemical analysis to determine the phenolic contents was also carried out. The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging, NCCLS broth microdilution and Plasmodium Lactate Dehydrogenase (pLDH) assays were used to determine antioxidant, antimicrobial and antiplasmodial activities, respectively. Haemolysis assay was conducted on A(+) human red blood cells and acute toxicity on male Swiss albino mice. Phenolics were quantitatively determined using spectrophotometric methods. The DPPH assay yielded interesting antioxidant activities of methanolic extract of Parinari curatellifolia (P. curatellifolia) and Entada africana (E. africana) (IC(50) were 0.20±0.01 μg/mL and 0.47±0.01 μg/mL, respectively). This activity was highly correlated with phenolic contents of extracts. The antimicrobial tests displayed minimal inhibitory concentrations (MICs) values ranging from 0.90 to 1.80 mg/mL for Serratia marcescens (S. marcescens) the most susceptible bacterial strain. MIC value was 1.20 mg/mL for susceptible fungal strains including Mucor rouxi (M. rouxi), Fusarium oxyporum (F. oxyporum) and Rhizopus nigricans (R. nigricans). pLDH assay showed moderate antiplasmodial activity of Balanites aegyptiaca (B. aegyptiaca) (IC(50) = 24.56±3.45 μg/mL), however this extract was highly haemolytic and toxic in mice (LD(50) = 625±128 mg/kg). Our results support in part the use of the selected plants in the treatment of microbial infections. In addition the plant showed interesting antioxidant activity that could be useful in the management of oxidative stress.
  Asian Pacific Journal of Tropical Medicine 10/2011; 4(10):808-13. · 0.37 Impact Factor
• Article: In vitro Anti Mycobacterium tuberculosis H37Rv Activity of Lannea acida A. Rich from Burkina Faso

  Ouattara L, Koudou J, Karou D.S, Giaco L, Capelli G, Simpore J, Fraziano M, Colizzi V, Traore A.S
  [show abstract] [hide abstract]
  ABSTRACT: The cytotoxic and anti-Mycobacterium tuberculosis H37Rv activities of hydro-alcoholic extract of Lannea acida A. Rich (Anacardiaceae) were assessed. The cytoxicity evaluation was carried out on THP1 monocytoid cell line (after 24 h at 1; 5 and 10 μg mL<SUP>-1</SUP>) and showed only a slight modification of lactate dehydrogenase (LDH) release. The rate of monocytes in different stages of mitosis had been amended in absence and presence of extract as follows: Go/ G1 58.83 - 59.83%; synthesis 21.95 - 18.64%; mitosis 16.67 - 15.77%; necrosis 2.65 - 5.64 %. The percentage of inhibition of Mycobacterium tuberculosis proliferation was respectively 77.6 and 36.8% at 1.2 and 0.6 mg mL<SUP>-1</SUP> of extract. This is an interesting experimental study on antimicrobial and immune-stimulating properties of Lannea acida ethanol-water (70%v/v) extract which may contain potential antibacterial and immune-stimulating agents for clinical use.
  Pakistan Journal of Biological Sciences. 01/2011;
• Source

  Available from: Zongo Cheikna

  Article: Antioxidant and Antibacterial Activities of Three Species of Lannea from Burkina Faso

  Ouattara L, Koudou J, Zongo C, Barro N, Savadogo A, Bassole I.H.N, Ouattara A.S, Alfred S. Traore
  [show abstract] [hide abstract]
  ABSTRACT: The main goal of this study was to determine the phenol content, the antibacterial and the antioxidant activities of the three species of Lannea largely use in traditional medicine in Burkina Faso. The total phenolic and flavonoid contents of hydro alcoholic extract (70%V/V ethanol/distilled water) from the barks of Lannea acida , Lannea microcarpa and Lannea velutina (Anacardiaceae) were determined by the method of Folin Ciocalteu and AlCl<SUB>3</SUB> by spectrophotometry. These extracts were tested for their antioxidant and antibacterial activities. Antioxidant activity was determined by the method of DPPH and compared with quercetin. Antibacterial activity was performed by disk diffusion and broth microdilution essays against nine reference bacterial strains including gram-positive and gram-negative bacteria. L.acida exhibited the highest total phenolic contents (40.55±0.26 g GAE/100 g) which correlated with better antioxidant activity (IC<SUB>50</SUB> = 345.72±7.76 ?g mL<SUP>-1</SUP>). Furthermore the highest content of total flavonoids (11.02±0.04 g QE/100 g) and the largest anti bacterial spectrum (7.82 ?g mL<SUP>-1</SUP>?MIC? 62.5 ?g mL<SUP>-1</SUP>) were recorded with L.velutina . These results show that the barks of L . acida and L.velutina could be used respectively as a potential natural antioxidant and antibacterial agent.
  Journal of Applied Sciences. 01/2011;
• Article: Polyphenols Content, Antioxidant and Antimicrobial Activities of Ampelocissus grantii (Baker) Planch. (Vitaceae): A Medicinal Plant from Burkina Faso

  Zongo C, Savadogo A, Ouattara L, Bassole I.H.N, Ouattara C.A.T, Ouattara A.S, Barro N, Koudou J, Traore A.S
  [show abstract] [hide abstract]
  ABSTRACT: The main goal of this study was to determine the phenol content, the antimicrobial and the antioxidant activities of acetone and water extracts from the rhizomes of Ampelocissus grantii (Baker) Planch (Vitaceae). The polyphenol content of extracts was determined colorimetrically using Folin-Ciocalteu method. The antioxidant activity of the same extracts was also screened using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The antimicrobial activity was performed by disc diffusion and broth microdilution assays against 9 reference bacterial strains including gram-negative and gram-positive bacteria and 1 fungal strain. The acetone extract exhibited the higher level of Total Polyphenol Contents (TPC) which correlated with strong antioxidant activity (IC<SUB>50 </SUB>= 10.87 μg mL<SUP>-1</SUP> and AAI = 5.6) and antimicrobial activity. The polyphenol content of the acetone extract was 124.26 gallic acid equivalent/100 g (GAE/100 g), that of water extract was 74.25 GAE/100 g. The largest diameter of inhibition zone (DIZ) (15 mm) and the lowest Minimal Inhibitory Concentration (MIC) value (0.625 mg mL<SUP>-1</SUP>) were recorded with Enterococcus faecalis 10907 CIP and Bacillus subtilus ATCC 21332. This study is a scientific base on the use of this plant in traditional health cure system.
  International Journal of Pharmacology. 01/2010;