Omaima Mohamed Kandil

Publications (27) View all

• Article: cDNA microarray analysis of gene expression in parthenotes and in vitro produced buffalo embryos.

  A S Abdoon, N Ghanem, O M Kandil, A Gad, K Schellander, D Tesfaye
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  ABSTRACT: The retarded development of parthenote embryo could be due to aberrant epigenetic imprinting, which may disrupt many aspects and lead to conceptus demise. The present work was conducted to: 1) compare the development of in vitro produced (IVP) and parthenogenetically developed (P) buffalo embryos from the 2-cell to blastocyst stage; 2) investigate the global gene expression profile and search for new candidate transcripts differing between IVP and P buffalo blastocyst using cDNA microarray analysis (validated by Real Time PCR); 3) follow the particular expression patterns of PLAC8 and OCT4 genes at five different stages of preimplantation development by Real Time PCR; and 4) study the expression of CDX2 at the blastcocyst stage. Cleavage rate was higher (P < 0.05) in P than IVP buffalo embryos, while, progression to blastocyst and number of cells per blastocyst were lower (P < 0.05) in P than IVP blastocysts. Microarray analysis indicate that 56 differentially expressed genes between the two groups, of which 51 genes (91.07%) were up-regulated, and five genes were downregulated in IVP blastocyst, using 1.4 fold-changes as a cutoff. Differentially expressed genes are related to translation, nucleic acid synthesis, cell adhesion and placentation. Validation of candidate genes revealed that the transcript abundance of PTGS2, RPS27A, TM2D3, PPA1, AlOX15, RPLO and PLAC8 were downregulated (7/8) in parthenote blastocyst compared to the IVP blastocyst. PLAC8 gene expression was higher (P < 0.05) at 2-cell, morula and blastocyst stages in IVP embryos compared with parthenote embryos. The OCT4 gene expression was higher (P < 0.05) in 2-cell, 4-cell, 8-cell and blastocysts produced in vitro. In conclusion, the retarded development of parthenogenetic buffalo embryos could be due to downregulation of genes related to translation, nucleic acid synthesis, cell adhesion, and placental development. The low expression of PLAC8 and OCT4 during the different stages of development may be responsible, in part, to the failure of development of parthenote buffalo embryos.
  Theriogenology 04/2012; 77(6):1240-51. · 1.96 Impact Factor
• Article: 110 EXPRESSION AND CLONING OF OESTRADIOL RECEPTOR α AND PROGESTERONE RECEPTORS AND INTERFERONE STIMULATED GENE 15 IN ENDOMETRIUM AND CORPUS LUTEUM OF PREGNANT CAMEL.

  A S Abdoon, O M Kandil, H Kleim, D Schams, B Berisha, C M Zeng
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  ABSTRACT: Despite their economic and cultural importance, dromedary camel is considered as a slow breeding animal, because of the higher incidence of early embryonic death. The present study was designed to investigate: 1) Expression and cloning of progesterone receptors (PR) and oestradiol receptor α (ERα) in CL and endometrium of pregnant camel; 2) Detection of interferon stimulated gene 15 (ISG15) in corpus luteum (CL) and endometrium of pregnant dromedary camels. For PR and ERα, RNA was extracted from CL and endometrium of dromedary camels during early (1 to 3 months), mid (4 to 9 months), and late stage (10 to 13 months) of pregnancy. Messenger RNA expression of PR and ERα was performed using RT-qPCR. Detection of ISG15 was performed using immunohistochemistry and Western blot analysis. In CL, both PR and ERα±showed the same pattern with significantly high (P<0.01) expression during early stage compared to mid or late stages of pregnancy. The lowest (P<0.01) expression was detected during the late stage of pregnancy compared with the mid stage. There was no difference inmRNA expression for PR and ERα in endometrium of during the different stages of pregnancy in dromedary camels. ISG15 conjugated protein showed no expression in CL or endometrium of pregnant dromedary camels either by immunohistochemistry or Western blot. In conclusion, PR and ERα potentially play a role in regulating luteal function in CL during pregnancy in dromedary camels, further work is necessary to study the mechanism of pregnancy recognition in dromedary camels.
  Reproduction Fertility and Development 01/2011; 23(1):160. · 2.11 Impact Factor
• Article: cDNA Microarray analysis of gene expression in parthenote and in vitro produced bufalo embryos

  Abdoon, A.S.S, N. Ghanem, O.M. Kandil, Schellander K, A. Gad, D. Tesfye
  Accepted in theriogenology journal, November,2011. 01/2011;
• Article: Mitochondrial distribution, ATP-GSH contents, calcium [Ca2+] oscillation during in vitro maturation of dromedary camel oocytes.

  Ahmed Sabry S Abdoon, Omaima M Kandil, Shen-Ming Zeng, Maosheng Cui
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  ABSTRACT: Dromedary camel oocytes have the ability to spontaneous parthenogenetic activation and development in vivo and in vitro. The present study was conducted to investigate changes in mitochondrial distribution, adenosine triphosphate (ATP), and glutathione (GSH) contents and [Ca(2+)] oscillation during in vitro maturation and spontaneous parthenogentic activation of dromedary camel oocytes. Dromedary camel cumulus-oocyte complexes (COCs) were matured in TCM199 medium supplemented with 10% FCS + 10 μg/mL FSH + 10 IU hCG + 10 IU eCG + 10 ng/mL EGF and 50 μg/mL gentamycine. Maturation was performed at 38.5 °C under 5% CO(2) in humidified air for 40 h. After maturation and removal of cumulus cells, oocytes were classified into: immature cultured (Group 1); metaphase II (M II, Group 2); and spontaneously parthenogenetically activated (with 2 polar bodies, Group 3); cleaved embryos (Group 4); and immature oocytes served as a control (Group 5). Cytoplasmic mitochondrial distribution, ATP-GSH contents, calcium [Ca(2+)] oscillation were determined. Results indicated that M II and spontaneously parthenogenetically activated oocytes represent 37.53% and 32.67% of the cultured oocytes, respectively, and 3.3% cleaved and developed to 2-16-cell stage embryos. Mitochondrial distribution, ATP-GSH contents and [Ca(2+)] oscillation were significantly (P < 0.01) differ between immature and matured dromedary camel oocytes. Mitochondrial distribution showed clustering form in matured oocytes without polar body. High polarized mitochondrial distribution (HPM) was detected in M II and spontaneously parthenogenetically activated oocytes, and the intensity of MitoTracker Red was higher in spontaneously parthenogenetically activated than M II. ATP-GSH contents and the duration of [Ca(2+)] oscillation were significantly (P < 0.01) higher in spontaneously parthenogenetically activated than M II oocytes or that matured without polar body. In conclusion, the higher incidence of spontaneously parthenogenetically activated in vitro matured dromedary camel oocytes could be attributed to the high polarized mitochondrial distribution associated with significantly higher ATP-GSH contents and duration of [Ca(2+)] oscillation.
  Theriogenology 08/2011; 76(7):1207-14. · 1.96 Impact Factor
• Article: Effect of Direct Exposure to Mancozeb Fungicide on the Developmental Competnce of Buffalo OocytesOocytes In vitro

  Abdoon A.S.S, Kandil O.M., Sabraa R.M.and Said A.A.
  Global Veterinaria 7 (3): 242-248, 2011. 01/2011; 7:242-248.