Article: Seasonal variability of intestinal helminths and Schistosoma haematobium in a rural area of the Sahel in Mali.H Niangaly, A A Djimde, B Traore, C P O Sangare, D Guindo, D Konate, M Diakite, N Diallo, O Maïga-Ascofare, N Sogoba, A Dabo, O K Doumbo[show abstract] [hide abstract]
ABSTRACT: Objective. The objective of this study was to determine the prevalence of intestinal helminths and Schistosoma haematobium before and after the rainy season in Pongonon, Mali. Methods. Volunteers aged one year and above were included. The Kato-Katz method was used to detect eggs and cysts in stool samples, and Wattman filtration to detect S. haematobium eggs in urine samples. Two cross-sectional surveys were conducted in July and November 2007. Results. In July (beginning of the rainy season), 304 volunteers were included; 278 were seen again in November (at the end of the rainy season). We found more intestinal helminths at the end of the rainy season (8.3%) compared to the beginning of the season (2.9%) (P = 0.01). There was no infection with S. haematobium in July but 7.6% in November (P < 0.001). The prevalence of intestinal helminths in children and adults was similar (P > 0.05), but the prevalence of infection with S. haematobium was higher in children aged 6 to 16 years (17/153) than in adults (2/74) (P = 0.02). Conclusion. Infections with helminth and S. Haematobium were both more prevalent at the end of the rainy season. Adults were infected as well as children and may constitute potential reservoirs of parasites. Effective control of these parasitic infections requires mass drug administration programs that take place during the seasons of high parasite egg excretion and that also include adult populations in some areas.Medecine et sante tropicales. 01/2013;
Article: Quinine Treatment Selects pfnhe1 ms47601 Polymorphism in Malian Patients with Falciparum Malaria.A Kone, J Mu, H Maiga, A A Beavogui, O Yattara, I Sagara, M M Tekete, O B Traore, A Dara, S Dama, N Diallo, A Kodio, A Traoré, A Björkman, J P Gil, O K Doumbo, T E Wellems, A A Djimde[show abstract] [hide abstract]
ABSTRACT: Background. The mechanism of Plasmodium falciparum resistance to quinine is not known. In vitro QTL mapping suggests involvement of a predicted P. falciparum sodiumhydrogen exchanger (pfnhe1) on chromosome 13.Methods. We conducted prospective quinine efficacy studies in two villages, Kollé and Faladié, Mali. Cases of clinical malaria requiring intravenous therapy were treated with standard doses of quinine and followed for 28 days. Treatment outcomes were classified using modified World Health Organization protocols. Molecular markers of parasite polymorphisms were used to distinguish recrudescent parasites from new infections. Prevalence of pfnhe1 ms47601 in infections before vs. after quinine treatment was determined by directsequencing.Results. Overall, 163 patients were enrolled and successfully followed. Without molecular correction, the mean ACPR was 50.3% (n = 163). After PCR correction to account for new infections, corrected ACPR was 100%. Prevalence of ms47601increased significantly from 26.2% (n = 107) before quinine treatment to 46.3% (n = 54) after therapy (P = 0.01). In a control sulfadoxinepyrimethamine study, prevalence of ms47601 was similar before and after treatment.Conclusions. This study supports a role for pfnhe1 in decreased susceptibility of P. falciparum to quinine in the field.The Journal of Infectious Diseases 11/2012; · 6.41 Impact Factor
A K Kone, P Delaunay, A A Djimdé, M A Thera, P D Giudice, D Coulibaly, K Traoré, S M Goita, A Abathina, A Izri, P Marty, O K Doumbo[show abstract] [hide abstract]
ABSTRACT: The epidemiology of the cutaneous leishmaniasis (CL) with Leishmania major is poorly documented in Mali. Following reports of CL in the tourist areas of the Dogon country (Bandiagara Escarpment), a joint French and Malian bio-clinical team conducted a field study from 16 to 27 January, 2010. The population of 5 villages has been examined by a dermato-infectiologist and cases were selected by visual inspection of skin lesions. Smears and biopsies (from the lesions) and venous blood were obtained from suspected cases of CL. Diagnosis was performed by light microscopy, in vitro cultures, serology and molecular biology. Fifty patients with skin lesions have been examined. Twenty-one have been suspected as CL. At least one sample was obtained from 18 patients. The lesions were predominantly old, more or less scarring and secondary infected. A skin smear was performed for 15 patients, a skin biopsy for 14 patients: smears and cultures were all negative. The PCR (Leishmania spp.) made on 14 biopsies was positive for 12 patients (86%). The low amount of amplified DNA obtained did not allow the sequencing and identification of the species of Leishmania. Western blot (WB) serology was positive in 11 cases out of 12 (92%). This investigation showed the presence of cutaneous leishmaniasis in Bandiagara. A further investigation is required during transmission period (September-October) to confirm the presence of Leishmania major epidemic in Dogon country.Bulletin de la Société de pathologie exotique 02/2012; 105(1):8-15.
D K Minta, A Dolo, M Dembele, A S Kaya, A T Sidibe, I Coulibaly, I I Maiga, M Diallo, A M Traore, M Y Maiga, O K Doumbo, H A Traore, E Pichard, D Chabasse[show abstract] [hide abstract]
ABSTRACT: Cryptococcal meningitis is the most common fatal central nervous system infection in AIDS patients in Sub-Saharan Africa. The purpose of this prospective study conducted from March 2003 to February 2004 in the internal medicine and infectious diseases departments of the Point G University Hospital Center was to investigate the clinical, prognostic and epidemiological profile of Cryptococcus neoformans infection in patients hospitalized for brain and meningeale infection (BMI). Diagnosis of neuromeningeal cryptococcosis (NMC) was based on positive identification of Cryptococcus by direct exam of the cebrospinal fluid (CSF) after India ink staining and/or culture on Sabouraud medium without actidione. During the study period, a total of 569 patients were hospitalized including 235 (41.3%) with HIV infection. Overall C. neoformans was identified in 14 patients. Median patient age was 39 +/- 8 years. There was a male preponderance with a sex ratio of 1.8 (9 men/5 women). Patients with BMI were HIV-positive in 85.7% of cases (n=12) and HIV-negative in 14.3% (n=2). The overall and HIV-specific prevalence of BMI was 2.5% and 5.1% respectively. The CD4 lymphocyte count was between I and 49 cells/mm3 in 64.3% of cases. The main clinical symptoms were cephalea in 85.7% of cases, altered consciousness in 50% and nausea/vomiting in 35.7%. Neurological manifestations (hemiparesis and cranial nerve deficit) were noted in 14.3%. HIV infection is the main purveyor of NMC in Mali. The actual incidence of cryptococcosis is unclear due to the poor sensitivity of diagnostic techniques. This study highlights diagnostic difficulties related to clinical polymorphism and poor technical facilities. Agglutination testing of blood and CSF is recommended, but mortality remains.Médecine tropicale: revue du Corps de santé colonial 12/2011; 71(6):591-5.
K E Lyke, M A Fernández-Viňa, K Cao, J Hollenbach, D Coulibaly, A K Kone, A Guindo, L A Burdett, R J Hartzman, A R Wahl, W H Hildebrand, O K Doumbo, C V Plowe, M B Sztein[show abstract] [hide abstract]
ABSTRACT: Pre-erythrocytic immunity to Plasmodium falciparum malaria is likely to be mediated by T-cell recognition of malaria epitopes presented on infected host cells via class I and II major histocompatibility complex (MHC) antigens. To test for associations of human leukocyte antigen (HLA) alleles with disease severity, we performed high-resolution typing of HLA class I and II loci and compared the distributions of alleles of HLA-A, -B, -C and -DRB1 loci in 359 Malian children of Dogon ethnicity with uncomplicated or severe malaria. We observed that alleles A*30:01 and A*33:01 had higher frequency in the group of patients with cerebral disease compared to patients with uncomplicated disease [A*30:01: gf = 0.2031 vs gf = 0.1064, odds ratio (OR) = 3.17, P = 0.004, confidence interval (CI) (1.94-5.19)] and [A*33:01: gf = 0.0781 vs gf = 0.0266, 4.21, P = 0.005, CI (1.89-9.84)], respectively. The A*30:01 and A*33:01 alleles share some sequence motifs and A*30:01 appears to have a unique peptide binding repertoire compared to other A*30 group alleles. Computer algorithms predicted malaria peptides with strong binding affinity for HLA-A*30:01 and HLA-A*33:01 but not to closely related alleles. In conclusion, we identified A*30:01 and A*33:01 as potential susceptibility factors for cerebral malaria, providing further evidence that polymorphism of MHC genes results in altered malaria susceptibility.Tissue Antigens 03/2011; 77(6):562-71. · 2.59 Impact Factor