Nurdan Pazarlioglu

Publications

• 0.69

  Impact points

  Molecularly imprinted polymers for some reactive dyes.

  Burcu Okutucu, Alper Akkaya, Nurdan Kasikara Pazarlioglu

  Preparative biochemistry & biotechnology. 10/2010; 40(4):366-76.

  Depending upon their structure, azo- and anthraquinonic dyes are the two major classes and together represent 90% of all organic colorants. Adsorption of dye molecules onto a sorbent can be an effective, low-cost method of color removal. Most of the techniques used for removal of dyes are of high pr... [more] Depending upon their structure, azo- and anthraquinonic dyes are the two major classes and together represent 90% of all organic colorants. Adsorption of dye molecules onto a sorbent can be an effective, low-cost method of color removal. Most of the techniques used for removal of dyes are of high production cost, and the regeneration also makes them uneconomical. There is much interest in the development of cheaper and effective newer materials for use as adsorbents. Molecular imprinting is a new kind of materials that can be alternative adsorbents. In this study, molecularly imprinted polymers of three textile dyes (Cibacron Orange P-4R, Cibacron Red P-4B, Cibacron Black PSG) were prepared. Methacrylic acid was used as a monomer for red and orange dyes and acrylamide was used for black dye. Methanol:acetonitrile was used as a porogen. The selective recognition ability of the molecularly imprinted polymers was studied by an equilibrium-adsorption batch method. The adsorption data are for Cibacron Black PSG 65% and nonimprinted polymer (NIP) 25%; Cibacron Red P-4B 72% and NIP 18%; and Cibacron Orange P-4R 45% and NIP 10%, respectively. Dye-imprinted polymers were used as a solid-phase extraction material for selective adsorption from wastewater of textile factory.
• 0.97

  Impact points

  Biodegradation of Direct Blue 15 by free and immobilized Trametes versicolor.

  Nurdan Kasikara Pazarlioglu, Alper Akkaya, Hatice Ardag Akdogan, Burcin Gungor

  Water environment research : a research publication of the Water Environment Federation. 07/2010; 82(7):579-85.

  To investigate biodegradability by Trametes versicolor, five structurally different direct azo-dyes--Direct Black 38, Direct Blue 15 (DB 15), Direct Orange 26, Direct Green 6, and Direct Yellow 12--were studied. The DB 15 was determined as the best biodegradable dye by this white-rot fungus. Laccase... [more] To investigate biodegradability by Trametes versicolor, five structurally different direct azo-dyes--Direct Black 38, Direct Blue 15 (DB 15), Direct Orange 26, Direct Green 6, and Direct Yellow 12--were studied. The DB 15 was determined as the best biodegradable dye by this white-rot fungus. Laccase and manganese peroxidase activities were monitored with the biodegradation process; it was observed that laccase played an important role in the dye degradation, while manganese peroxidase activity could not be detected. Possible degradation products also were examined by gas chromatography-mass spectrometry, but no metabolite was detected after the degradation and/or decolorization process. To enhance performance of the fungi during the degradation, Trametes versicolor cells were immobilized in alginate beads. Then, DB 15 decolorization by immobilized Trametes versicolor was studied in a small-scale packed-bed reactor. The color removal efficiency in repeated batches was found to be 98 and 93% for 50 mg/L DB 15.
• 0.69

  Impact points

  Pyranose 2-oxidase (P2O): Production from Trametes versicolor in Stirred Tank Reactor andits Partial Characterization.

  Nurdan Kasikara Pazarlioglu, Alper Akkaya, Dilek Tahsinsoy

  Preparative biochemistry & biotechnology. 02/2009; 39(1):32-45.

  Optimization of pyranose-2-oxidase (P2O) production conditions from Trametes versicolor was carried out in shaking cultures containing glucose, malt, and yeast extracts; the optimum concentration values were found to be 1.5% glucose, 1.0% yeast extract, and 1.0% malt extract, pH 5.0, temperature, 26... [more] Optimization of pyranose-2-oxidase (P2O) production conditions from Trametes versicolor was carried out in shaking cultures containing glucose, malt, and yeast extracts; the optimum concentration values were found to be 1.5% glucose, 1.0% yeast extract, and 1.0% malt extract, pH 5.0, temperature, 26 degrees C, and agitation rate 150 rpm. For the first time, P2O production was also carried out in a stirred tank reactor (STR) with 2.2 L working volume in the optimized medium composition, and biomass, P2O activity, protein, nitrogen and glucose concentrations were also monitored besides pH and dissolved oxygen (DO). In the STR, P2O activity peaked on day 9. Partial enzyme characterization occurred and optimum pH and temperature were detected as 7.0 and 37 degrees C, respectively. K(m) value was found to be 1.009 mM.
• 0.69

  Impact points

  Manganese peroxidase production by immobilized Phanerochaete chrysosporium BKM-F-1767 in a cell bioreactor.

  Raziye Ozturk Urek, Nurdan Kasikara Pazarlioglu

  Preparative biochemistry & biotechnology. 02/2008; 38(1):1-12.

  Manganese-dependent peroxidase (MnP) production was performed in an immobilized cell bioreactor in which Phanerochaete chrysosporium BKM-F-1767 was immobilized on polystyrene foam. The immobilized cell culture yielded significantly greater MnP activity than the conventional stationary liquid culture... [more] Manganese-dependent peroxidase (MnP) production was performed in an immobilized cell bioreactor in which Phanerochaete chrysosporium BKM-F-1767 was immobilized on polystyrene foam. The immobilized cell culture yielded significantly greater MnP activity than the conventional stationary liquid culture. Cultivation was carried out in batch mode; the effect of glucose concentration was investigated and growth kinetics parameters were found as, micromax=0.59 day(-1), Ks=0.33 g/L and Kss=14.5. Batch operation led to maximum MnP (770.82 U/L) in the culture medium containing 0.05% Tween 80, 10 g/L glucose, and 174 microM Mn2+ at 37 degrees C and pH 4.5. Enzyme productivity was obtained as 110.12 U/day/L.
• 3.29

  Impact points

  Determination of phenolic acids using Trametes versicolor laccase.

  Dilek Odaci, Suna Timur, Nurdan Pazarlioglu, Maria Rita Montereali, Walter Vastarella, Roberto Pilloton, Azmi Telefoncu

  Talanta. 02/2007; 71(1):312-7.

  Two biosensors based on Trametes versicolor laccase (TvL) were developed for the determination of phenolic compounds. Commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. The systems were calibrated for three phenolic ... [more] Two biosensors based on Trametes versicolor laccase (TvL) were developed for the determination of phenolic compounds. Commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. The systems were calibrated for three phenolic acids. Linearity was obtained in the concentration range 0.1-1.0muM caffeic acid, 0.05-0.2muM ferulic acid, 2.0-14.0muM syringic acid for laccase immobilised on a commercial oxygen electrode and 2.0-30.0muM caffeic acid, 2.0-10.0muM ferulic acid, 4.0-30.0muM syringic acid for laccase immobilised on ferrocene-modified screen-printed electrodes. Furthermore, optimal pH, temperature and thermal stability studies were performed with the commercial oxygen electrode. Both electrodes were used for determination of a class of phenolic acids, achieving a cheap and fast tool and an easy to be used procedure for screening real samples of human plasma.
• 0.94

  Impact points

  Laccase biosensors based on mercury thin film electrode.

  Ulkü Anik Kirgöz, Hüseyin Tural, Suna Timur, Nurdan Pazarlioglu, Azmi Telefoncu, Roberto Pilloton

  Artificial cells, blood substitutes, and immobilization biotechnology. 02/2005; 33(4):447-56.

  A biosensor was developed by immobilizing laccase onto mercury thin film electrode (MTFE) by means of gelatin that is then crosslinked with glutaraldehyde. Mercury thin film (MTF) was deposited onto glassy carbon electrode (GCE) and the obtained biosensor was utilized for the determination of phenol... [more] A biosensor was developed by immobilizing laccase onto mercury thin film electrode (MTFE) by means of gelatin that is then crosslinked with glutaraldehyde. Mercury thin film (MTF) was deposited onto glassy carbon electrode (GCE) and the obtained biosensor was utilized for the determination of phenolic compounds. The measurement was based on the amperometric detection of oxygen consumption in relation to analyte oxidation. The optimum experimental conditions for the biosensor were investigated and the system was calibrated for both catechol and phenol. A linear relationship between sensor responses and analyte concentrations was obtained in concentration range between 0.5 x 10(-6)-5.0 x 10(-6)M for catechol and 2.5 x 10(-6)-2.0 x 10(-6)M for phenol, respectively. Mercury thin film was also formed onto the surface of screen printed graphite electrodes and applied for the catechol detection. The linearity was observed in concentration range between 2.5 x 10(-6)-3.0 x 10(-5)M.

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• Biodecolourization of Direct Blue 15 by immobilized Phanerochaete chrysosporium

  Nurdan Kasikara Pazarlioglu, Raziye Ozturk Urek, Fulya Ergun

  Process Biochemistry.

  In vitro and in vivo biodecolourization of structurally different nine direct azo dyes by Phanerochaete chrysosporium immobilized on ZrOCl2-activated pumice was studied in stationary cultures. No lignin peroxidase activity was detected in the extracellular medium of P. chrysosporium. In order to sup... [more] In vitro and in vivo biodecolourization of structurally different nine direct azo dyes by Phanerochaete chrysosporium immobilized on ZrOCl2-activated pumice was studied in stationary cultures. No lignin peroxidase activity was detected in the extracellular medium of P. chrysosporium. In order to support dye degradation, ligninolytic culture filtrate from fungus, containing mainly manganese peroxidase, was treated with dye. Direct Blue 15 (DB15, 120 mg/l) was determined as the best decolourized dye and its decolourization by immobilized P. chrysosporium was studied in a small-scale packed-bed reactor (PBR). The colour removal efficiency in repeated batches was found as 95–100%. Kinetic analysis of enzymatic decolourization of DB15 indicate that the process is time dependent and follows first-order kinetics with respect to initial concentrations of dye. The rates of colour removal (k values) decrease to a significant extent with increasing initial concentrations of dye. In this decolourization process, it was observed that MnP played an important role while there was no obvious role for LiP and adsorption was determined as a minor mechanism in decolourizing DB15.

• Determination of phenolic acids using Trametes versicolor laccase

  Dilek Odaci, Suna Timur, Nurdan Pazarlioglu, Maria Rita Montereali, Walter Vastarella, Roberto Pilloton, Azmi Telefoncu

  Talanta.

  Two biosensors based on Trametes versicolor laccase (TvL) were developed for the determination of phenolic compounds. Commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. The systems were calibrated for three phenolic ... [more] Two biosensors based on Trametes versicolor laccase (TvL) were developed for the determination of phenolic compounds. Commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. The systems were calibrated for three phenolic acids. Linearity was obtained in the concentration range 0.1–1.0 μM caffeic acid, 0.05–0.2 μM ferulic acid, 2.0–14.0 μM syringic acid for laccase immobilised on a commercial oxygen electrode and 2.0–30.0 μM caffeic acid, 2.0–10.0 μM ferulic acid, 4.0–30.0 μM syringic acid for laccase immobilised on ferrocene-modified screen-printed electrodes. Furthermore, optimal pH, temperature and thermal stability studies were performed with the commercial oxygen electrode. Both electrodes were used for determination of a class of phenolic acids, achieving a cheap and fast tool and an easy to be used procedure for screening real samples of human plasma.