Answer added in Agricultural Science21 How can I measure antioxidant enzyme activities?By Zhu Lulu · Northeast Agricultural UniversityNori Talamoni · University of Cordoba (Spain)Please, see our papers in BBActa 2008, J. Nutrional Biochem. 2003(Marchionatti A et al.). We measured SOD, CAT and GPX in chick intestine. We have not... [more]Please, see our papers in BBActa 2008, J. Nutrional Biochem. 2003(Marchionatti A et al.). We measured SOD, CAT and GPX in chick intestine. We have not worked with tomatoes.Following
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ABSTRACT: Calcium is essential for bone and tooth formation, achievement of optimal peak bone mass and also for regulation of physiological processes. The calcium demand depends on age, gender and different physiological processes. These requirements are higher during childhood, pregnancy and lactation. Dietary Ca2+ deficiency modifies Ca2+ homeostasis and the metabolism of calciotropic hormones and increases the efficiency of intestinal Ca2+ absorption and renal reabsorption, altering bone metabolism. The low Ca2+ diet is associated with hypertension and risk of cancer.Revista de la Facultad de Ciencias Médicas (Córdoba, Argentina) 06/2009; 66(2):66-72.
Article: Calcium and phosphorous deficiencies alter the lipid composition and fluidity of intestinal basolateral membranes.N G Tolosa de Talamoni[show abstract] [hide abstract]
ABSTRACT: Steady-state fluorescence polarization and lipid composition studies were undertaken on intestinal basolateral membranes (BLM) from chicks adapted to a calcium deficient (low Ca) or a phosphorous deficient diet (low P). The fluorescence anisotropy showed that fluidity of intestinal BLM was increased by the mineral deprivations, but the response of the membranes varied with the specific fluorophore used. The "static" component of fluidity, assessed by 1,6-diphenyl-1,3,5-hexatriene (DPH), was increased whereas the "dynamic" component, monitored with DL-12-(9-anthroyloxy)-stearic acid (12-AS), was not modified. Low P diet produced significant changes in lipid composition such as a decrease in the cholesterol content and in the sphyngomyelin (Sph) and phosphatidylserine plus phosphatidylinositol fractions (PS + PI) and increment in the phosphatidylcholine (PC) proportion. The percent of monounsaturated fatty acids was increased by the low P diet due mainly to an increase in the oleic acid fraction. Minor changes such as a decrease in the palmitic acid and increases in the 22:5n3 and 22:6n3 fatty acids were caused by Ca deficiency. The alteration of the biochemical and biophysical membrane properties of the BLM of the mineral deficient groups might play a role in the enhanced intestinal Ca and P absorption.Comparative biochemistry and physiology. Part A, Physiology 01/1997; 115(4):309-15.
Article: New perspectives in melatonin uses.[show abstract] [hide abstract]
ABSTRACT: This review summarizes the metabolism, secretion, regulation and sites of action of melatonin. An updated description of the melatonin receptors, including their signal transduction mechanisms, distribution and characterization of receptor genes, is given. Special emphasis is focused on the clinical aspects and potential uses of melatonin in the sleep-wake rhythms, in the immune function, in cancer therapy, in neuroprotection against oxidative damage and antioxidant activities in different tissues. Finally, combined effects of melatonin with other drugs are discussed.Pharmacological Research 01/2012; 65(4):437-44. · 4.44 Impact Factor
Article: Effect of retinal ablation on the expression of calbindin D28k and GABA in the chick optic tectum.[show abstract] [hide abstract]
ABSTRACT: The effect of retinal ablation on qualitative and quantitative changes of calbindin D28k and GABA expression in the contralateral optic tectum was studied in young chicks. Fifteen days old chicks had unilateral retinal ablation and after 7 or 15 days, calbindin expression was analyzed by Western blot and immunocytochemistry. Neuronal degeneration was followed by the amino-cupric silver technique. After 15 days, retinal lesions produced a significant decrease in calbindin immunostaining in the neuropil of layers 5-6 and in the somata of neurons from the layers 8 and 10 of the contralateral tectum, being this effect less marked at 7 days post-lesion. Double staining revealed that 50-60% of cells in the layers 8 and 10 were calbindin and GABA positive, 30-45% were only calbindin positive and 5-10% were only GABAergic neurons. Retinal ablation also produced a decrease in the GABA expression at either 7 or 15 days after surgery. At 7 days, dense silver staining was observed in the layers 5-6 from the optic tectum contralateral to the retinal ablation, which mainly represented neuropil that would come from processes of retinal ganglion cells. Tectal neuronal bodies were not stained with silver, although some neurons were surrounded by coarse granular silver deposits. In conclusion, most of calbindin molecules are present in neurons of the tectal GABAergic inhibitory circuitry, whose functioning apparently depends on the integrity of the visual input. A possible role of calbindin in the control of intracellular Ca2+ in neurons of this circuit when the visual transmission arrives to the optic tectum remains to be studied.European journal of histochemistry: EJH 02/2003; 47(4):365-72. · 1.69 Impact Factor
Article: Comparative immunolocalization of the plasma membrane calcium pump and calbindin D28K in chicken retina during embryonic development.[show abstract] [hide abstract]
ABSTRACT: The immunolocalization of the plasma membrane calcium pump (PMCA) was studied in 4-week-old chick retina in comparison with calbindin D28K (CaBP) immunostaining. We have demonstrated that the monoclonal anti-PMCA antibody SF10 from human erythrocyte plasma membrane cross-reacts with a Ca2+ pump epitope of the cells from the neural retina. The immunolocalization of both proteins was also studied during the embryonic development of the chicken retina. At age 4.5 days, the cells of the retina were faintly immunoreactive to PMCA and CaBP antibodies, but the lack of cellular aggregation and differentiation did not allow discrimination between the two proteins. A clear difference in the localization was seen from the tenth day of development through post-hatching with slight variation. PMCA localized mainly in the outer and inner plexiform layers, in some cells in the ganglion layer, in the nerve fiber layer and slightly in the photoreceptor cells. CaBP was intensely stained in cones, cone pedicles and some amacrine cells. The number of CaBP positive amacrine cells declined after hatching. A few ganglion cells and several nerve fibers were CaBP immunoreactive. The role of these proteins in the early stages of retinal development is unknown, but the results suggest that Ca2+ homeostasis in the retina is well regulated, probably to avoid excessive accumulation of Ca2+, which often leads to neurodegeneration.European journal of histochemistry: EJH 02/2002; 46(4):333-40. · 1.69 Impact Factor