Nidhi Kumar

Questions and Answers (10) View all

• Answer added in Microbial Biotechnology
  11 What is the role of calcium chloride (CaCl2) in the lysis buffer?
  By Henrique Bianchi · Universidade Estadual de Campinas
  Nidhi Kumar · The Oxford Educational Institutions

  Calcium chloride or even sodium chloride,(which is more commonly used in a lysis buffer) increases the osmoloarity(the total of solute particles per l... [more]

  Calcium chloride or even sodium chloride,(which is more commonly used in a lysis buffer) increases the osmoloarity(the total of solute particles per liter of a solution) of the buffer solution containing the cells as water can diffuse from cells(hypotonic environment) to a hypertonic environment(buffer solution), thereby aiding in faster lysis of cells.
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• Answer added in Probiotics
  7 What is the easiest way of isolating and purifying bacteriocin?
  By Josiah Lennox · University of Calabar
  Nidhi Kumar · The Oxford Educational Institutions

  Sir, you are absolutely right, I just forgot to mention that in my reply! 

  Sir, you are absolutely right, I just forgot to mention that in my reply!
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• Answer added in Probiotics
  7 What is the easiest way of isolating and purifying bacteriocin?
  By Josiah Lennox · University of Calabar
  Nidhi Kumar · The Oxford Educational Institutions

  1) Propogate the Lactobacilli MRS media for 3 days using a shaker incubator. 2) The culture media is subjected to centrifugation at 4°C for 20 min.... [more]

  1) Propogate the Lactobacilli MRS media for 3 days using a shaker incubator. 2) The culture media is subjected to centrifugation at 4°C for 20 min. 3) Preciptate the Cell Free Supernatent(CFS) using 85% ammonium sulphate. 4) Again centrifuge the cell free supernatent at 4°C for 35 min. 5) Go for the appropriate purification technique, usually ion- exchange chromatography is preferred. In case of non-lanthionine bacteriocins, ion exchange chromatogrpahy using a octyl-sepharose CL-4B (Octyl Sepharose™ CL-4B is a separation medium for hydrophobic interaction chromatography (HIC). Substances are separated on the basis of their different hydrophobicity. The octyl group is covalently coupled to a crosslinked 4% agarose matrix by ether linkage, giving a hydrophobic gel with minimal leakage and no ionic properties.) and reverse phase HPLC
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• Answer added in Chromatography
  22 Thin layer chromatography (TLC) with protein
  By Thomas Lake · The University of Sheffield
  Nidhi Kumar · The Oxford Educational Institutions

  I agree with Menaga, if it is butanol acetic acid and water, then it is mostly used in the ratio 4:1:5(by volume) 

  I agree with Menaga, if it is butanol acetic acid and water, then it is mostly used in the ratio 4:1:5(by volume)
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• Answer added in Chromatography
  22 Thin layer chromatography (TLC) with protein
  By Thomas Lake · The University of Sheffield
  Nidhi Kumar · The Oxford Educational Institutions

  Dear Tom, y don't u try with cyclodextrin instead of silica gel?. I know cyclodextrins r used in HPLC columns for seperating chiral enantiomers, 

  Dear Tom, y don't u try with cyclodextrin instead of silica gel?. I know cyclodextrins r used in HPLC columns for seperating chiral enantiomers,
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