Publications (184) View all
-
Article: Pulsed-field gel electrophoresis analysis of Bordetella pertussis isolates circulating in Europe in 1998 to 2009.
[show abstract] [hide abstract]
ABSTRACT: Between 1998 and 2009 Bordetella pertussis clinical isolates were collected during three periods: 1) 1998-2001 (n=102), 2) 2004-2005 (n=154), 3) and 2007-2009 (n=140) from nine countries with distinct vaccination programs: Denmark, Finland, France, Germany, The Netherlands, Norway, Poland, Sweden, and the United Kingdom. Pulsed-field gel electrophoresis (PFGE) analysis was performed according to standardized recommendations for epidemiological typing of B. pertussis. There were 81 different PFGE-profiles, five of which (BpSR3, BpSR5, BpSR10, BpSR11 and BpSR12) were observed in 61% of the 396 isolates and shown to be predominant in almost all countries. The major profile, BpSR11 showed a decreasing trend from 25%-30% in 1998-2005 to 13% in 2007-2009, and an increase was observed in BpSR3 and BPSR10 from 0% and 8% to 21% and 22% respectively. One difference noticed between these profiles is that BpSR11 contains isolates harboring fim3-2 allele and BpSR3 and BpSR10 isolates harboring fim3-1 allele. The total proportion of the five predominant profiles increased from 44% in 1998-2001, 63% in 2004-2005 to 70% in 2007-2009. In conclusion, common PFGE profiles were identified in B. pertussis populations circulating in European countries with different vaccination programs and different vaccine coverage. These prevalent isolates contain the novel pertussis toxin promoter ptxP3 allele. However, there is evidence for diversifying selection between ptxP3 strains characterized by distinct PFGE profiles. This work shows that, even within a relative short time span of 10 years, successful isolates may emerge which spread through Europe and cause large shifts in B. pertussis populations.Journal of clinical microbiology 11/2012; · 4.16 Impact Factor -
Article: Appearance of Bordetella pertussis Strains Not Expressing the Vaccine Antigen Pertactin in Finland.
[show abstract] [hide abstract]
ABSTRACT: Despite the extensive vaccinations, resurgence of pertussis has been reported in many countries (9).…Clinical and vaccine immunology: CVI 08/2012; 19(10):1703-4. · 2.37 Impact Factor -
Article: Reply to "Bordetella holmesii in Nasopharyngeal Samples from Chilean Patients with Suspected Bordetella pertussis Infection".
Journal of clinical microbiology 04/2012; 50(4):1506. · 4.16 Impact Factor -
Article: Prevalence of Bordetella pertussis and Bordetella parapertussis infections in Tunisian hospitalized infants: results of a 4-year prospective study.
[show abstract] [hide abstract]
ABSTRACT: The prevalence of pertussis in Tunisia remains undetermined essentially because of the unavailability of a basic laboratory diagnostic service. Specific diagnostic tools were applied for the first time in a Tunisian prospective study in order to get a first estimation of the prevalence of Bordetella pertussis/parapertussis infections and to evaluate their use to determine the epidemiologic characteristics of these infections in Tunisian infants. Between 2007 and 2011, a total of 626 samples from 599 infants aged <1 year with and without pertussoid cough were investigated for the presence of B. pertussis/parapertussis using culture and real-time polymerase chain reaction (PCR). The real-time PCR (RT-PCR) targets include IS481 commonly found in B. pertussis, B. bronchiseptica, and B. holmesii; IS1001 specific of B. parapertussis, in combination with the pertussis toxin promoter region gene (ptx) of B. pertussis; and the recA gene specific of B. holmesii. When possible, patients' household contacts provided nasopharyngeal aspirates (NPAs) for RT-PCR detection of B. pertussis/parapertussis or single-serum samples for anti-PT IgG quantification. All except 1 NPAs were negative by conventional culture, whereas PCR gave positive signals for 126 specimens (21%): B. pertussis, B. parapertussis, and Bordetella spp. were detected in 82%, 6%, and 4% of the samples, respectively. The simultaneous presence of B. pertussis and B. parapertussis was noted in 8% of the cases. Pertussis was reported throughout the year with a peak during the summer of the year 2009. The prevalence of Bordetella infection was 20% between 2007 and 2011. Most of these cases corresponded to patients younger than 6 months who received <3 doses of pertussis vaccine. Among the household contacts enrolled in the study, mothers seemed to be the likely source of infection. This study showed that pertussis is still prevalent in Tunisia and that the disease remains a public health problem affecting not only infants but also adults. Given this situation, sensitive and specific laboratory tests are needed to improve the accuracy of pertussis diagnosis.Diagnostic microbiology and infectious disease 02/2012; 72(4):303-17. · 2.45 Impact Factor -
Article: Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis.
[show abstract] [hide abstract]
ABSTRACT: Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS481, which is not specific for Bordetella pertussis. We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.Journal of clinical microbiology 12/2011; 49(12):4347-8. · 4.16 Impact Factor
