Nathalie Steimberg

Questions and Answers (7) View all

• Answer added in Skeletal Muscle
  6 What connexins/pannexins are expressed in skeletal muscle?
  By Nicolas Pillon · SickKids
  Nathalie Steimberg · Università degli Studi di Brescia

  I know only C2C12. They express cx43.but not cx40 and in litterature, I saw that they express also cx39 

  I know only C2C12. They express cx43.but not cx40 and in litterature, I saw that they express also cx39
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• Answer added in Skeletal Muscle
  6 What connexins/pannexins are expressed in skeletal muscle?
  By Nicolas Pillon · SickKids
  Nathalie Steimberg · Università degli Studi di Brescia

  in skeletal muscle, Cx43, Cx45 and Cx39 are expressed. The level of expression depends on several factors such as health of tissue and state of differ... [more]

  in skeletal muscle, Cx43, Cx45 and Cx39 are expressed. The level of expression depends on several factors such as health of tissue and state of differentiation (for example from myoblasts to myotubes), tissue regeneration..... You should as indicated by James Nagy & Eric Beyer perform a Pubmed research, because you will find number of articles/reviews that could help you.
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• Answer added in Liver Cancer
  5 Which protein is expressed in mouse liver, but not in human?
  By Milad Rezvani · University of California, San Francisco
  Nathalie Steimberg · Università degli Studi di Brescia

  I think as Luiz that it will be difficult to find a protein specifically expressed in mouse rather than in human. In addition to look for specie-speci... [more]

  I think as Luiz that it will be difficult to find a protein specifically expressed in mouse rather than in human. In addition to look for specie-specific antibody, and according to your experimental aim, you could look at for protein differently expressed at the quantitative level (or example connexins (cxs26 and 32)) or differently induced (ex: CYP450).
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• Answer added in Animal Cell Culture
  11 Do you routinely use antibiotics in culture media? What do you use?
  By Yong Lin · University of Virginia
  Nathalie Steimberg · Università degli Studi di Brescia

  Using antibiotics depend on your objective. Less you use better is. The choice of the antibiotics you will use, will depend on the protection you want... [more]

  Using antibiotics depend on your objective. Less you use better is. The choice of the antibiotics you will use, will depend on the protection you want to give: Wide range of action antibiotics More restricted range of action. It also depends on which kind of cells your are working primary cells from animal, human…or cell lines. Of course for cell selection it exists other types of antibiotics, but they are different and their use is more targeted. When using antibiotics for the first time on a peculiar cell type, it is better to test their toxicity on this cell type.
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• Answer added in Cell Culture
  19 I tried to recover skin fibroblast cells, which were stored in liquid Nitrogen for 2 years. But the cells are not growing. Does anyone have any suggestions?
  By Pratibha Aryal · Mahidol University
  Nathalie Steimberg · Università degli Studi di Brescia

  I believe that quickly thaw of frozen cells at 37°C is necessary, as well as slowly centrifugate to completely remove DMSO, in addition, perhaps you ... [more]

  I believe that quickly thaw of frozen cells at 37°C is necessary, as well as slowly centrifugate to completely remove DMSO, in addition, perhaps you could try : 1) 20% FBS 2) seeding cells at higher density (you can choose: 2 vials in one T75, or 1 vial in 1 T25 Sometimes after several years some cells are difficult to recover and dermal fibroblast characteristics vary from a donor to another one. I never had problem with dermal fibroblasts, perhaps you had a problem with N2? or freezing steps? before doing another isolation of cells you should ascertain that all these steps and your container are ok. by end, are you sure of your plasticware? did you change the lot number? are you alone in your lab with this problem? Good luke
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