Nagarajan Perumal

Publications (19) View all

• Article: Effect of long-term castration on serum biochemistry in rhesus monkeys.

  P Nagarajan, S Arindkar, S Singh, S S Majumdar
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  ABSTRACT: BACKGROUND: Testicular failure has an effect on normal physiology. To address this issue, an experimental non-human primate model of long-term castrated rhesus monkey was chosen for this study to evaluate the influence of castration on various biochemical parameters. METHODS: Nine castrated rhesus monkeys were evaluated for changes in body weight, serum testosterone, and serum biochemical parameters as compared to those in non-castrated macaques. RESULTS: Castration caused statistically significant changes in body weight, biochemical analytes, and testosterone levels. Body weight and testosterone levels were decreased, and there were increase in alanine aminotransferase, cholesterol, serum bilirubin, phosphorous, alkaline phosphatase, urea and a decrease in serum protein, uric acid, creatinine, and triglycerides. CONCLUSIONS: This study provided essential baseline information on biochemical variables due to the effect of castration associated with declining levels of testosterone, as data are not readily accessible from the existing body of scientific literature on non-human primates.
  Journal of Medical Primatology 03/2013; · 1.30 Impact Factor
• Article: Tamoxifen-resistant, ER-positive MAC 51 cell line with a high metastatic potential developed from a spontaneous breast cancer mouse model.

  Jerald Mahesh Kumar, Ponvijay Kombairaju, P Nagarajan, Thanumalayan Subramanian, Jedy Jose, Hullathy Subban Ganapathy, Takeo Ohsugi
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  ABSTRACT: We developed and characterized an estrogen-responsive and ER-positive murine breast cancer cell line (MAC51) from a spontaneous breast cancer animal model. These cells are overexpressed with K8, K18 and K19 proteins in an immunofluoresence assay. Upregulation of ER alpha was observed in the immunofluoresence assay, real-time PCR analysis and western blot assay. A colocalization experiment in MAC 51 showed cytoplasmic colocalization of K18 and K19 proteins with ER α. Real-time analysis of tumor samples from engrafted animals, MAC 51, metastatic liver and metastatic ovary revealed overexpression of K8 and K18 compared to the respective controls. A hormone responsive experiment in immunodeficient mice showed highly significant decreases in estrogen and tumor volume after 14 days ovariectomization. The tumorogenicity assay showed higher (3 × 10 (5)) and lower (3 × 10(4)) concentrations of MAC 51 cells that developed tumors within 2 weeks post-transplantation. Tumor morphology and histology resembled a sarcoma pattern but our spontaneous model appeared in an adenocarcinoma pattern. Metastasis to different organs occurred through hematogenous and lymphatic routes. We assessed the potency of the anticancer effect in MAC 51 cells by treating various anticancer drugs with E2, followed by studying apoptotic gene expression profiles. E2 and E2+ tamoxifen-treated cells showed upregulation of apoptotic genes caspase 1, 3, 9, P53 and Bcl-xl but the tamoxifen- and paclitaxel-treated cells did not upregulate the apoptotic genes. Tamoxifen-resistant, ER-positive and high metastatic potential cell lines from murine origin are very rare. Also, estrogen greatly induced apoptosis in this cell line, hence MAC 51 has a greater application potential to evaluate low doses of estrogen with other targeted therapeutic drugs to treat breast cancer.
  Cell and Tissue Research 08/2012; 350(2):347-60. · 3.11 Impact Factor
• Article: The Effect of Fasting on Haematology Serum Biochemistry Parameters on STZ Induced CD1 Mice and Diabetic db/db Mice

  Shailendra Arindkar, Mahesh kumar MJ, Ramesh C Juyal, Subeer S Majumdar and Nagarajan Perumal
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  ABSTRACT: http://www.omicsonline.org/2157-7609/2157-7609-3-137.digital/2157-7609-3-137.html Modest information is available on how fasting affects clinical pathology variables in (Streptozocin) STZ induced diabetes mice and db/db mice. This study was carried out to evaluate the influence of fasting on clinical pathology variables in diabetic mouse models. Seven male STZ induced diabetic CD1 mice and db/db mice were fasted for 18 hours and change in body weight (BW), hematologic and biochemical variables were evaluated. Fasting provoked significant variation in body weight, haematology and biochemical variables in diabetic animal models. The results suggested that clinical pathology variables will vary after fasting. The decision to feed or fast before blood collection for analyzing the results should be made based on fasting in animal models for diabetic research.
  J Drug Metab Toxicol. 12/2012;
• Article: Antigen Peptide Transporter 1 (TAP 1-/- ) is involved in the development of fructose-induced hepatic steatosis in mice.

  Shailendra A, Jashdeep B, Jerald MK, Barun D, Upadhyay P, Asif S, Ramesh CJ, Subeer SM, Perumal N
  Journal of Gastroenterology and Hepatology 01/2013; · 2.87 Impact Factor
• Article: Nagarajan, P., Arindkar, S., Singh, S. and Majumdar, S.S. (2013), Effect of long-term castration on serum biochemistry in rhesus monkeys. Journal of Medical Primatology. doi: 10.1111/jmp.12046

  Nagarajan, Arindkar, Singh, S.S. Majumdar
  Journal of Medical Primatology 01/2013; · 1.30 Impact Factor