Muhammed em |
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PhD scholar in Animal Breeding
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Kerala Veterinary and Animal Sciences University
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Department of Animal Breeding, Genetics & Biostatistics
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Skills (5)
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99 Questions5556 Followers
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23 Questions3301 Followers
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3 Questions17 Followers
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157 Questions12259 Followers
Education
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Sep 2010
Kerala Veterinary and Animal Sciences University
Vechur cattle, Molecular biology · PhDIndia · Thrissur -
Sep 2008–
Mar 2010Kerala Veterinary and Animal Sciences University
Molecular biology · MVScIndia · Thrissur
Other
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LanguagesEnglish
Questions and Answers (31) View all
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Answer added in Gene Expression39 English language usage: gene expression or gene transcription in describing RT-PCR results?By Felix Toka · Szkoła Główna Gospodarstwa WiejskiegoMuhammed em · Kerala Veterinary and Animal Sciences UniversityI feel gene transcription would be suitable.I think we can can also consider mRNA profile which would be more specific.I feel gene transcription would be suitable.I think we can can also consider mRNA profile which would be more specific.Following
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Answer added in Bioinformatic Tools20 Primer design for real time PCRBy Jesica Canizo · Instituto Nacional de Tecnología AgropecuariaMuhammed em · Kerala Veterinary and Animal Sciences UniversityI design the qRT PCR primer with primer 3 and test it with sequence manipulation suite(SMS).Then , Check the primer efficiency in gel. For qRTPCR, si... [more]I design the qRT PCR primer with primer 3 and test it with sequence manipulation suite(SMS).Then , Check the primer efficiency in gel. For qRTPCR, since we design primers for 100-200 bp product, we get good primers in the first step itself.Primers were working well when other conditions were optimum.In my opinion, we have to concentrate more on preparation of good quality RNA and cDNA as failure of qRTPCR were mostly associated with problems in RNA isolation or cDNA preparation.Good luck JesicaFollowing
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Answer added in mRNA7 mRNA stabilityBy Abdul Q. Sheikh · Yale UniversityMuhammed em · Kerala Veterinary and Animal Sciences UniversityI think you can try qRT PCR with that RNA. If RNA later was added what would have been the condition of Abdul'S RNA?I think you can try qRT PCR with that RNA. If RNA later was added what would have been the condition of Abdul'S RNA?Following
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Answer added in PCR3 Why should the RNA concentration be the same in different samples at the step of cDNA synthesis?By Srikanta Chowdhury · University of ChittagongMuhammed em · Kerala Veterinary and Animal Sciences UniversityThank you Ros M D for providing valuable information. I am presently doing qRT PCR and this is very useful for meThank you Ros M D for providing valuable information. I am presently doing qRT PCR and this is very useful for meFollowing
Publications (2) View all
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Article: THERMAL STRESS IN DAIRY CATTLE 12 3 4
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ABSTRACT: Crosses of temperate cattle reared in tropical ambience lapse in heat tolerance, fertility and disease resistance. However, cross breeding zebu with high yielding exotic cattle seems necessary to meet the need for enhanced composite milk production per unit in view of food security. Thermal stress is considered as the main factor responsible for reduction of milk yield in tropical climate. Any model for the study of thermal stress in dairy cattle should encompass related effect of ambient parameters of temperature, humidity, wind speed and solar radiation as the stressor and behavioural, autonomic, neuroendocrine and immunological endpoints as responses. The responses reach different response states depending on the severity of the stressor. Response measurements are at behavioural, physiological, biochemical and cellular level, which also include hormone, protein and gene expression assays. By fitting these measurements to the described model we can work out the biological and economic cost of thermal stress and the level of adaptation of the dairy animal in question. Such studies taking in to consideration the diverse nature of climatic factors is imperative for finding ameliorative measures to reduce the thermal stress experienced by the existing cattle population and for the possible genetic and management strategies for evolving and maintaining a climatically adapted dairy stock in a state like Kerala. This review analyses suitable model for climatic adaptation studies in the hot and humid climate especially in small holder production systems.JIVA. 12/2012; 10(3):45-51. -
SourceAvailable from: Muhammed em
Article: BETA CASEIN A1A2 POLYMORPHISM AND MILK YIELD IN VECHUR, KASARGODE DWARF AND CROSSBRED CATTLE* 1 2
E M Muhammed, M Stephen[show abstract] [hide abstract]
ABSTRACT: Beta Casein (â-CN) is the second most abundant protein in cow's milk that contains 209 amino acids. Among the twelve â-CN variants identified A1 and A2 are the common types and others are very rare. A1 variant has histidine at position 67 of the amino acid sequence while A2 possess proline at this position-this polymorphism has attracted much public health attention, since consumption of A1 milk is reported to cause various illness. The high producing Bos taurus cattle are found to possess more A1 allele. The present study was undertaken to assess the relationship between â-CN A1/A2 polymorphism with milk yield in Vechur, Kasargode and Crossbred cattle. The average daily milk yield was 1.27±0.05 kg in Vechur cattle and the peak yield of crossbred cattle was 10.71±1.046 kg. The average daily milk yield for A1A2 genotype in Vechur cattle was 1.52±0.08 kg and 1.14±0.04 kg for A2A2 genotype and the difference in the milk yield of the two genotypes was significantly different (p=0.05). In crossbred cattle the peak yield (kg) of A1A1 genotype was 14.64±3.181, 8.54±0.194 for A1A2 genotype and 9.09±0.125 for A2A2 genotype. The A1A1 genotype in crossbred cattle showed significantly higher average peak yield compared to other genotypes and difference between A1A2 and A2A2 genotypes were not significantly different (p=0.05). Thus it can be concluded that selection for enhancing milk production may increase the frequency of harmful A1 allele in bovine population.JIVA. 12/2012; 10(`3):5-9.