Mohammad Reza Bolouri

Questions and Answers (9) View all

• Answer added in Western Blot
  21 Western blot problem
  By Parveen Sarkari · Heinrich-Heine-Universität Düsseldorf
  Mohammad Bolouri · Shahid Beheshti University of Medical Sciences

  I think it may related to protein accumulation especially when your protein is cysteine rich you can use reduced agent like DTT to evaluate this 

  I think it may related to protein accumulation especially when your protein is cysteine rich you can use reduced agent like DTT to evaluate this
  Following
• Answer added in Transfection
  10 Problem transfecting 4T1 breast cancer cell line
  By Robinot Rémy · Université François Rabelais
  Mohammad Bolouri · Shahid Beheshti University of Medical Sciences

  I had done the same work on 4t1 cells.I transfected with pEGFPN1 plasmid containing EGFP as monitoring marker by lipofectamin plus reagent .The first ... [more]

  I had done the same work on 4t1 cells.I transfected with pEGFPN1 plasmid containing EGFP as monitoring marker by lipofectamin plus reagent .The first assy when I was done by FlowCytometry for GFP detection, I had 10% positive cells, but because I needed stable cell line, I used selection marker G418 and step by step outcomes became better and finally I reached 98% positive cell clone with good MFI for GFP expression.so I think if you want to increase your positive cell(if stable cell line you want) you should select your cells by selective marker like Antibiotics and if you want transiant expression its better to set different amount of lipofectamin and plasmid to reach the best I also suggest PolyEthylenimine tranfection protocol that was published by nature protocol2012 as altarnative method By best
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• Answer added in Autophagy
  2 First french meeting dedicating to autophagy
  By pierre lapaquette · Institut Pasteur International Network
  Mohammad Bolouri · Shahid Beheshti University of Medical Sciences

  Sorry! will you have english version of meeting?! 

  Sorry! will you have english version of meeting?!
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• Question asked in Autophagy
  Open Autophagy and Antigen presentation on MHC class I (from Autophagy and immunity work group)
  There is not conclusive evidences about autophagy's role in antigen presentation by MHC class I but we have conclusive evidences about cross presentat... [more]
  There is not conclusive evidences about autophagy's role in antigen presentation by MHC class I but we have conclusive evidences about cross presentation so, its reasonable that autophagy has the same role in MHC class I antigen presentation as MHC class II What are you think about this statement?
  By Mohammad Bolouri · Shahid Beheshti University of Medical Sciences
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• Answer added in Apoptosis
  18 Does anyone knows what happends regarding the apoptosis process once I put the cells in ice? does it stops? proceeds?
  By Tal Etzyoni · Tel Aviv University
  Mohammad Bolouri · Shahid Beheshti University of Medical Sciences

  The cold is the foe of apoptosis but the warm is the friends of apoptosis! 

  The cold is the foe of apoptosis but the warm is the friends of apoptosis!
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