Mira Mladen Pucarević

Publications

• 4.14

  Impact points

  Heavy metals, organics and radioactivity in soil of western Serbia.

  Goran Dugalic, Dragana Krstic, Miodrag Jelic, Dragoslav Nikezic, Biljana Milenkovic, Mira Pucarevic, Tijana Zeremski-Skoric

  Journal of hazardous materials. 05/2010; 177(1-3):697-702.

  Western Serbia is a region well-known for potato production. Concentrations of selected metals, polycyclic aromatic hydrocarbons (PAHs) and radioactivity were measured in the soil in order to evaluate the quality and characteristics. The examined soils (Luvisol and Pseudogley) showed unsuitable agro... [more] Western Serbia is a region well-known for potato production. Concentrations of selected metals, polycyclic aromatic hydrocarbons (PAHs) and radioactivity were measured in the soil in order to evaluate the quality and characteristics. The examined soils (Luvisol and Pseudogley) showed unsuitable agrochemical characteristics (acid reaction, low content of organic matter and potassium). Some samples contained Ni, Mn and Cr above the maximal permissible concentration (MPC). The average concentration of total PAHs was 1.92 mg/kg, which is larger than the maximal permissible concentration in Serbia but below the threshold values in the European Union for food production. The average radioactivity of (238)U, (226)Ra, (232)Th, (40)K and the fission product (137)Cs were 60.4+/-26.2, 33.2+/-13.4, 49.1+/-18.5, 379+/-108 and 36.4+/-23.3 Bq/kg. Enhanced radioactivity in the soils was found. The total absorbed dose rate in air above the soil at 1m height calculated for western Serbia was 73.4 nGy/h and the annual effective dose was 90 microSv, which are similar to earlier reports for the study region.

• Determination of trans fatty acids in foodstuffs by gas chromatography–mass spectrometry after simultaneous microwave-assisted extraction–esterification - Supplementary material

  KRAVIĆ SNEŽANA, SUTUROVIĆ ZVONIMIR, ŠVARC-GAJIĆ JAROSLAVA, STOJANOVIĆ ZORICA, PUCAREVIĆ MIRA

  Journal of the Serbian Chemical Society. 01/2010;

  Supplementary material

• Determination of trans fatty acids in foodstuffs by gas chromatography–mass spectrometry after simultaneous microwave-assisted extraction–esterification

  KRAVIĆ SNEŽANA, SUTUROVIĆ ZVONIMIR, ŠVARC-GAJIĆ JAROSLAVA, STOJANOVIĆ ZORICA, PUCAREVIĆ MIRA

  Journal of the Serbian Chemical Society. 01/2010;

  A sample preparation method based on the simultaneous microwave-assisted extraction–esterification (SMAEE) was developed for the determination of the fatty acid composition of foodstuffs by gas chromatography–mass spectrometry. The proposed sample preparation method was validated by comparison with ... [more] A sample preparation method based on the simultaneous microwave-assisted extraction–esterification (SMAEE) was developed for the determination of the fatty acid composition of foodstuffs by gas chromatography–mass spectrometry. The proposed sample preparation method was validated by comparison with the reference Soxhlet extraction method followed by derivatisation by ester formation and the same determination step. The fatty acid compositions and the extraction efficiencies obtained using the proposed SMAEE method and the reference method was statistically similar. The results showed that compared to the conventional method, the SMAEE method offered the advantages of short sample preparation time, low consumption of expensive organic solvents and lower energy consumption. This good agreement between results provided by both the SMAEE and the reference method demonstrates the usefulness of the former as a routine method for the treatment of food samples prior to trans fatty analysis.
• 0.51

  Impact points

  Determination of the Trans Fatty Acid Content of Serbian Shortenings by Gas Chromatography-mass Spectrometry

  Kravic Snezana Z Marjanovic Nikola J Suturovic Zvonimir J Svarc-Gajic Jaroslava V Stojanovic Zorica S Pucarevic Mira

  Acta Alimentaria. 01/2010; 39:413-423.

• Sulfonylurea tolerance of wheat genotypes in zygotic embryo culture

  Ankica Kondić-Špika, Kristina Petrović, R. Jevtić, B. Kobiljski, Mira Pucarević

  Archives of Biological Sciences. 01/2009;

  Tolerance of wheat genotypes to the sulfonylurea herbicide metsulfuron-methyl was studied using in vitro culture. Six randomly selected wheat genotypes were used for isolation. Mature embryos were inoculated onto a modified MS medium to which three concentrations of metsulfuron-methyl were added: I-... [more] Tolerance of wheat genotypes to the sulfonylurea herbicide metsulfuron-methyl was studied using in vitro culture. Six randomly selected wheat genotypes were used for isolation. Mature embryos were inoculated onto a modified MS medium to which three concentrations of metsulfuron-methyl were added: I-30 g l-1, II-60 g l-1, and III-90 g l-1. The control group of embryos was cultivated on a herbicide-free medium. After one month of cultivation, callus fresh weight was measured. There were significant differences among the genotypes regarding their tolerance to metsulfuron-methyl. The Pobeda cultivar expressed the highest and the Lr-12 genotype had the lowest level of herbicide tolerance.

• Genetic specificity of magnesium nutrition in sunflower

  Kastori Rudolf R, Marinković Radovan Z, Sekulić Petar Đ, Maksimović Ivana V, Pucarević Mira M

  Zbornik Matice Srpske za Prirodne Nauke. 01/2008;

  Magnesium content was analyzed in five of the most grown sunflower hybrids in Serbia, as well as in different populations of wild sunflower species: Helianthus agrophyllus (5), Helianthus annuus (4), Helianthus neglectus (3), Helianthus petiolaris (5), Helianthus tuberosus (5). Magnesium content in ... [more] Magnesium content was analyzed in five of the most grown sunflower hybrids in Serbia, as well as in different populations of wild sunflower species: Helianthus agrophyllus (5), Helianthus annuus (4), Helianthus neglectus (3), Helianthus petiolaris (5), Helianthus tuberosus (5). Magnesium content in the populations of wild sunflower species ranged from 317 to 824 mg/100 g DW. The highest magnesium content was found in Helianthus petiolaris and the lowest in Helianthus tuberosus. Different populations within each species differed significantly in magnesium content. The highest variation coefficient was found in Helianthus tuberosus and the lowest in Helianthus petiolaris. Magnesium concentration in hybrids was significantly different as well, and in two years it was in average between 575 and 813 mg/g DW. The results suggest that genetic variability between magnesium concentrations in wild species and hybrids of sunflower is very high. This should be taken into consideration when requirements for mineral nutrition are analyzed, as well as when wild species are included in breeding programs.
• 1.19

  Impact points

  EVALUATION OF QUALITY PARAMETERS OF TABLE BEET VARIETIES IN BABY BEET PRODUCTION

  Takács-Hájos M. Szabó L., Kastori R., Pucarević M., Zeremski-Škorić T.

  Cereal Research Communications. 01/2008; 36:1075-1079.

• Determination of polycyclic aromatic hydrocarbons in soil by gas chromatography-mass spectrometry

  Kravić Snežana Ž, Marjanović Nikola J, Pucarević Mira M, Suturović Zvonimir J, Švarc-Gajić Jaroslava V

  Acta Periodica Technologica. 01/2005;

  Polycyclic aromatic hydrocarbons (PAHs) were determined in soil samples from forty different locations in Vojvodina. The samples were Soxhlet extracted cleaned-up and analyzed using gas chromatography-mass spectrometry (GC-MS) in the selected ion monitoring mode (SIM). Each PAH was separately quanti... [more] Polycyclic aromatic hydrocarbons (PAHs) were determined in soil samples from forty different locations in Vojvodina. The samples were Soxhlet extracted cleaned-up and analyzed using gas chromatography-mass spectrometry (GC-MS) in the selected ion monitoring mode (SIM). Each PAH was separately quantified using an eight-point calibration of mixed standard solutions in the range between 0.25 and 10 μg/cm3. The sum of the 16 US Environmental Protection Agency PAHs analyzed in the soil samples varied from 0.043 to 1.362 mg/kg of dry soil.

• Polycyclic aromatic hydrocarbons and pesticides in soil of Vojvodina

  Pucarević Mira M, Sekulić Petar Đ

  Zbornik Matice Srpske za Prirodne Nauke. 01/2004;

  The paper deals with several groups of compounds that represent the most frequent pollutants of soil in the world. The paper also reviews results of long-term studies conducted at the Institute of Field and Vegetable Crops in Novi Sad on the residues of pesticides and polycyclic aromatic hydrocarbon... [more] The paper deals with several groups of compounds that represent the most frequent pollutants of soil in the world. The paper also reviews results of long-term studies conducted at the Institute of Field and Vegetable Crops in Novi Sad on the residues of pesticides and polycyclic aromatic hydrocarbons (PAHs) in the soil of the Vojvodina Province. The analyzed samples have been found to contain residues of persistent pesticides and their metabolites lindane and its metabolites 6,20 μg/kg, alachlor 3,56 μg/kg, aldrin 2,3 μg/kg, heptachlor epoxide 0,99 μg/kg, chlordane 3,82 μg/kg, DDT and its metabolites 10,77 μg/kg, dieldrin 2,04 μg/kg, endrin 3,57 μg/kg and endrin aldehyde 1,36 μg/kg. Soil samples from Novi Sad municipality contained 53,69 μg/kg of DDT and its metabolites. The values of atrazine ranged from 0,0005 to 0,8 mg/kg. The values of PAHs were 6,64 mg/kg in industrial soil, 4,93 mg/kg in agricultural soil, and 4,55 mg/kg and 5,48 mg/kg in the Novi Sad municipality. The lowest value, 0.83 mg/kg, was found for nonagricultural/nonindustrial soils.
• 4.36

  Impact points

  Atrazine in groundwater of Vojvodina Province.

  Mira Pucarević, Radmila Sovljanski, Sanja Lazić, Nikola Marjanović

  Water research. 01/2003; 36(20):5120-6.

  The objective of this study was to investigate concentrations of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine), deethylatrazine (DEA) (2-amino-4-chloro-6-isopropylamino-1,3,5-triazine), deisopropylatrazine (DIA) (2-amino-4-chloro-6-ethylamino-1,3,5-triazine) and deethyldeisopropyl... [more] The objective of this study was to investigate concentrations of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine), deethylatrazine (DEA) (2-amino-4-chloro-6-isopropylamino-1,3,5-triazine), deisopropylatrazine (DIA) (2-amino-4-chloro-6-ethylamino-1,3,5-triazine) and deethyldeisopropylatrazine (DEIA) (6-chloro-2,4-diamino-1,3,5-triazine) in groundwaters of Vojvodina Province. A study was conducted during April 2001. Some 110 samples of groundwater were taken from near surface aquifers. The water samples were first passed through a disk containing solid matrix coated with a chemically bonded C-18 organic phase. The disk was then eluted with supercritical carbon dioxide to remove compounds from the sorbent. Finally the extract was injected into capillary gas chromatograph. Average concentrations were 0.198 microg L(-1) for atrazine, 0.116 microg L(-1) for DEA, 0.043 microg L(-1) for DIA and 0.077 microg L(-1) for DEIA.
• 0.98

  Impact points

  Effect of Brestan on Saccharomyces cerevisiae during continuous cultivation.

  R Razmovski, M Pucarević

  Folia microbiologica. 02/2002; 47(5):507-10.

  An increase in Brestan concentration in nutrient media decreased the content of protein, phosphorus, total ribonucleic acid, activity of pyruvate carboxylase and isocitrate lyase in cells of Saccharomyces cerevisiae parent strain and respiratory deficient (RD) mutant while the trehalose content incr... [more] An increase in Brestan concentration in nutrient media decreased the content of protein, phosphorus, total ribonucleic acid, activity of pyruvate carboxylase and isocitrate lyase in cells of Saccharomyces cerevisiae parent strain and respiratory deficient (RD) mutant while the trehalose content increased. The respiration quotient value for the RD mutant was higher than for the parent strain. The RD mutant lacked cytochrome aa3; cytochrome c and b contents were lower than those of the parent strain.

• Atrazine in groundwater of Vojvodina Province

  Mira Pucarević, Radmila Šovljanski, Sanja Lazić, Nikola Marjanović

  Water Research.

  The objective of this study was to investigate concentrations of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine), deethylatrazine (DEA) (2-amino-4-chloro-6-isopropylamino-1,3,5-triazine), deisopropylatrazine (DIA) (2-amino-4-chloro-6-ethylamino-1,3,5-triazine) and deethyldeisopropyl... [more] The objective of this study was to investigate concentrations of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine), deethylatrazine (DEA) (2-amino-4-chloro-6-isopropylamino-1,3,5-triazine), deisopropylatrazine (DIA) (2-amino-4-chloro-6-ethylamino-1,3,5-triazine) and deethyldeisopropylatrazine (DEIA) (6-chloro-2,4-diamino-1,3,5-triazine) in groundwaters of Vojvodina Province. A study was conducted during April 2001. Some 110 samples of groundwater were taken from near surface aquifers. The water samples were first passed through a disk containing solid matrix coated with a chemically bonded C-18 organic phase. The disk was then eluted with supercritical carbon dioxide to remove compounds from the sorbent. Finally the extract was injected into capillary gas chromatograph. Average concentrations were 0.198 μg L−1 for atrazine, 0.116 μg L−1 for DEA, 0.043 μg L−1 for DIA and 0.077 μg L−1 for DEIA.