Mattia Gallizioli |
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MSc Medical Biotechnology
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Università Vita-Salute San Raffaele
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Department of Neurology
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Skills (11)
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528 Questions26001 Followers
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109 Questions17615 Followers
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877 Questions47592 Followers
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39 Questions89 Followers
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29 Questions689 Followers
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159 Questions2267 Followers
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22 Questions33 Followers
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3 Questions28 Followers
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0 Questions10 Followers
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2 Questions39 Followers
Research experience
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Apr 2013–
presentResearch: BBB characterisation in aged mice after stroke
Ospedale di San Raffaele Istituto di Ricovero e Cura a Carattere Scientifico · Institute of Experimental Neurology INSPE · Neuroimmunology UnitItaly · Milano -
Sep 2011–
Mar 2013Research: Trainee - BBB in ageing mice
Ospedale di San Raffaele Istituto di Ricovero e Cura a Carattere Scientifico · Neuroimmunology UnitItaly · Milano
Education
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Sep 2010–
Mar 2013Università Vita-Salute San Raffaele
Cellular and Molecular Medical Biotechnology · MScItaly · Milan -
Sep 2007–
Sep 2010Università Vita-Salute San Raffaele
Medical and Pharmaceutical Biotechnology · BScItaly · Milano
Other
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LanguagesItalian, English
Questions and Answers (5) View all
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Answer added in Immunofluorescence Microscopy2 Does anybody know a good protocol for alpha-SMA immunofluorescent staining on pericytes in brain tissue?By Mattia Gallizioli · Università Vita-Salute San RaffaeleMattia Gallizioli · Università Vita-Salute San RaffaeleI would like to see aSMA to have information on contractility of the cells. I need to do it on tissue...do you have any advice? thanksI would like to see aSMA to have information on contractility of the cells. I need to do it on tissue...do you have any advice? thanksFollowing
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Question asked in Immunofluorescence Microscopy2 Does anybody know a good protocol for alpha-SMA immunofluorescent staining on pericytes in brain tissue?I have fresh frozen brain tissue sections cut with cryostat at 12 um and 60 um PFA fixed sections. I use ms anti-SMA antibody A-2547 by Sigma but I ca... [more]I have fresh frozen brain tissue sections cut with cryostat at 12 um and 60 um PFA fixed sections. I use ms anti-SMA antibody A-2547 by Sigma but I can't get it right.By Mattia Gallizioli · Università Vita-Salute San RaffaeleFollowing
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Answer added in Neuroscience8 Is there a way to identify exactly which cell population is expressing a gene, which I can see is overexpressed, in brain tissue?By Mattia Gallizioli · Università Vita-Salute San RaffaeleMattia Gallizioli · Università Vita-Salute San Raffaeleis there any database to look up? I already have some "candidates", and I would like to spare time and money on IF and other experiments. Is it possib... [more]is there any database to look up? I already have some "candidates", and I would like to spare time and money on IF and other experiments. Is it possible? Thank you very muchFollowing
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Question asked in Neuroscience8 Is there a way to identify exactly which cell population is expressing a gene, which I can see is overexpressed, in brain tissue?I see an increase of different laminins under particular conditions in brain tissue, but I don't know where they come from.I see an increase of different laminins under particular conditions in brain tissue, but I don't know where they come from.By Mattia Gallizioli · Università Vita-Salute San RaffaeleFollowing