Mary Grace B Saldajeno

Publications

• 0.94

  Impact points

  Interaction between the plant growth-promoting fungus Phoma sp. GS8-2 and the arbuscular mycorrhizal fungus Glomus mosseae: impact on biocontrol of soil-borne diseases, microbial population, and plant growth

  Mary Grace B. Saldajeno, Masanori Ito, Mitsuro Hyakumachi

  Australasian Plant Pathology. 01/2012;

  The influence of the interaction between Phoma sp. GS8-2 (GS8-2) and the arbuscular mycorrhizal fungus Glomus mosseae (Gm) on the development of soil-borne diseases, microbial population, and plant growth in cucumber, bentgrass and tomato plants was investigated under controlled conditions. The comp... [more] The influence of the interaction between Phoma sp. GS8-2 (GS8-2) and the arbuscular mycorrhizal fungus Glomus mosseae (Gm) on the development of soil-borne diseases, microbial population, and plant growth in cucumber, bentgrass and tomato plants was investigated under controlled conditions. The composite inoculation of GS8-2 and Gm yielded varied effects on suppression of symptoms of soil-borne pathogens depending on the host-pathogen combination involved. The GS8-2 + Gm had neutral effect in reducing damping-off disease of cucumber caused by Rhizoctonia solani AG-4 and the crown and root rot disease of tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici (Forl), but exacerbated the brown patch disease caused by R. solani AG2-2 in bentgrass. In tomato plants, Forl populations in the roots and rhizosphere were significantly reduced by GS8-2 + Gm. Results also show that the composite inoculation of GS8-2 and Gm resulted to a synergistic effect on the reduction of fungal and bacterial populations in roots and rhizosphere of the plants. Plant growth enhancement was due to the individual effect of GS8-2 or Gm but not their interaction. GS8-2 root colonization of cucumber and tomato plants decreased significantly in dual inoculated plants compared to plants inoculated with GS8-2 alone; while no significant differences were found in the Gm root colonization of the three plant species indicating that GS8-2 had no effect on Gm.

• The plant growth-promoting fungus Fusarium equiseti and the arbuscular mycorrhizal fungus Glomus mosseae stimulate plant growth and reduce severity of anthracnose and damping-off diseases in cucumber (Cucumis sativus L.) seedlings

  Saldajeno M.G.B., M. Hyakumachi

  Annals of Applied Biology. 01/2011; 159:28-40.

• Arbuscular Mycorrhizal Interactions with Rhizobacteria or Saprotrophic Fungi and its Implications to Biological Control of Plant Diseases

  M. G. B. Saldajeno, M. Hyakumachi

  01/2011: pages 187-212;

  ISBN: 978-1-61122-659-1

• Effects of Interactions of Arbuscular Mycorrhizal Fungi and Beneficial Saprophytic Mycoflora on Plant Growth and Disease Protection

  M. G. B. Saldajeno, W. A. Chandanie, M. Kubota, M. Hyakumachi

  01/2008: pages 211-226;

  ISBN: 978-1-4020-8769-1 (Print) 978-1-4020-8770-7 (Online)

• Production of antiserum of abaca mosaic potyvirus using antigen prepared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)

  Saldajeno, M.G.B., N. B. Bajet

  The Philippine Agriculturist. 01/1998; 81:77-88.

  A procedure was developed to purify abaca mosaic potyvirus (AMV) capsid proteins from field-infected abaca and to produce antiserum for AMV detection. Eight samples of abaca leaves showing varying degrees of mosaic symptoms were found to be AMV-infected in indirect ELISA using an antiserum against s... [more] A procedure was developed to purify abaca mosaic potyvirus (AMV) capsid proteins from field-infected abaca and to produce antiserum for AMV detection. Eight samples of abaca leaves showing varying degrees of mosaic symptoms were found to be AMV-infected in indirect ELISA using an antiserum against sugarcane mosaic potyvirus, strain H (SCMV-H) at 1:1000 dilution. Total protein extracts from the leaf samples were electrophoresed in 15 percent. SDS-PAGE and an AMV-specific protein of molecular weight 34.5 KD (P34) was identified. This P34 protein is likely to be the coat protein as it was the major protein band that was detected by Western blot analysis using the SCMV-H antiserum. These results provide evidence and further confirm previous results that the virus causing mosaic of abaca is serologically related to SCMV. Gel bands containing the proteins were collected, homogenized and mixed (1:1) with Freund's incomplete adjuvant and the emulsion injected to a rabbit. The antiserum collected had a homologous titer of 1/1026 when the rabbit was sacrificed. At a dilution of 1:512, the crude antiserum produced high A405 nm readings (0.194-0.468 nm) in indirect ELISA with the mosaicked abaca maintained in the greenhouse and with mosaicked corn leaves from the field. Absorbances of 0.007-0.152 nm were obtained with healthy abaca, corn and banana and with abaca with bunchy-top symptoms. The crude antiserum was able to detect its homologous antigen in western blots at a dilution of 1:10,000. These results demonstrate the applicability of SDS-PAGE to isolate and purify antigens to produce antiserum for detecting viruses in plants.