Skills (20)
Education
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Jan 1992–
Jul 1998Technische Universität Kaiserslautern
Biology · Ph.D.Germany · Kaiserslautern
Other
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LanguagesEnglish, German, Italian
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Other InterestsAfter my PhD in Biology, working as software and web developer now.
My research related websites: www.nenno.it/Beanref/, www.nenno.it/karyotypedb/, www.nenno.it/Beanseedimages/
www.nenno.it/phaseolus-polytene-chromosomes/
Publications (5) View all
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Article: Chromosomal localization and distribution of simple sequence repeats and the Arabidopsis-type telomere sequence in the genome of Cicer arietinum L.
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ABSTRACT: We used fluorescence in situ hybridization to probe the physical organization of five simple sequence repeat motifs and the Arabidopsis-type telomeric repeat in metaphase chromosomes and interphase nuclei of chickpea (Cicer arietinum L.). Hybridization signals were observed with the whole set of probes and on all chromosomes, but the distribution and intensity of signals varied depending on the motif. On root-tip metaphase chromosomes, CA and GATA repeats were mainly restricted to centromeric areas, with additional GATA signals along some chromosomes. TA, A and AAC repeats were organized in a more dispersed manner, with centromeric regions being largely excluded. In interphase nuclei of the inner integument, CA and GATA signals predominantly occurred in the heterochromatic endochromocentres, whereas the other motifs were found both in eu- and heterochromatin. The distribution of the Arabidopsis-type telomeric repeat (TTTAGGG)n on metaphase chromosomes was found to be quite exceptional. One major cluster of repeats was spread along the short arm of chromosome B, whereas a second, weaker signal occurred interstitially on chromosome A. Only faint and inconsistent hybridization signals were visualized with the same probe at the chromosomal termini.Chromosome Research 02/1998; 6(2):97-104. · 3.09 Impact Factor -
Thesis: Charakterisierung der Polytänchromosomen aus dem Embryosuspensor von Phaseolus coccineus L.
07/1998, Degree: PhD, Supervisor: PD Dr. H. Scherthan, Prof. Dr. H. J. Schmidt -
Article: Localization of different microsatellites and a minisatellite-like sequence on polytene chromosomes of Phaseolus coccineus
Annu. Rep. Bean Imp. Coop. [BIC]. 01/1996; 39:245-246. -
Article: Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment.
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ABSTRACT: This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Genome 01/1995; 37(6):1018-21. · 1.65 Impact Factor -
Article: Mapping phaseolin genes to polytene chromosomes by fluorescence in situ hybridization (FISH)
About
After my PhD in Biology, working as software and web developer now.
