Article: Abd-Elmaksoud, A. S., S. M. Ebada & M. B. Shoaib, 2012. Localisation of cytokeratin and smooth muscle actin in the accessory genital glands of camels (Camelus dromedarius) during rutting and non-rutting seasons. Bulg. J. Vet. Med., 15, No 4, 213-227.[show abstract] [hide abstract]
ABSTRACT: The present study has disclosed for the first time the distribution of cytokeratin (CK) and α smooth muscle actin (αSMA) in the accessory genital glands of camel. In prostate, CK was localised in the cytoplasm of columnar cells of secretory acini and in the scanty cytoplasm of basal cells. In the ampulla of ductus deferens, the CK reaction was found in the pseudostratified columnar epithelium of mucosa and in the secretory columnar epithelium of submucosal glands. In the bulbourethral gland, CK reaction was exclusively observed in the pyramidal cells of type A and type C secretory units as well as in the lining epithelium of the duct system. αSMA was localised to the smooth muscle cells of the prostatic capsule, fibromuscular stroma and blood vessels. In the ampulla, αSMA reaction was seen in the smooth muscle of tunica muscularis, fibromuscular stroma and blood vessels. In the bulbourethral gland, α SMA was only localised to the smooth muscle cells of the capsule and blood vessels in both reproductive periods. Unexpectedly, neither the interlobular nor the intralobular connective tissue stroma of bulbourethral gland has reacted to αSMA. In conclusion, the distribution of CK and α SMA in the accessory genital glands of camels might point out to their roles in the male reproduction.Bulgarian journal of Veterinary Medicine. 12/2012; 15(4):213-224.
Article: Some anatomical studies on the muscles of the antebrachium and manus regions of Eastern Gray Kangaroo (Macropus Giganteus)Shoaib M.B[show abstract] [hide abstract]
ABSTRACT: Eastern grey kangaroo is a large herbivorous marsupial animal found in southern and eastern areas of Australia. It belongs to Family Macropodidae .The present investigation was carried out on the carcasses of eight adult eastern gray kangaroos for studying the gross anatomy of the muscles of the antebrachium and manus regions. The antibrachial muscles classified into: A-dorso-lateral group which comprise; Mm. extensor carpi radialis, extensor carpi ulnaris, extensor digitorum communis, extensor digitorum lateralis, extensor digiti I and extensor carpi obliquus. Functionally, these muscles represented the extensors for the carpal and digital joints. In addition to M. brachioradialis and supinator which located in the flexor angle of the elbow joint and serve to turn the forepaw around the long axis. B- Palmar group which comprise; Mm. flexor carpi radialis, flexor carpi ulnaris, palmaris longus, flexor digitorum superficialis and profundus. In addition to Mm. pronator teres and pronator quadratus which rotate the forearm medially. Functionally, these muscles represented the flexors of the carpal and digital joints. In conclusion, the antebrachium and manus regions in eastern gray kangaroo resemble nearly that of other macropod and carnivores in general, however, some original differences were detected between them in this study. Key word: anatomy, muscles, antebrachium and manus, eastern grey kangarooJ.Vet.Anat. 01/2009; 2(1-1):1-15.
Article: Morphology of the stomach of Eastern Gray Kangaroo (Macropus Giganteus) with special reference to its sacciform fore stomach.Shoaib M.B[show abstract] [hide abstract]
ABSTRACT: Nine adult Eastern gray kangaroos were used to study the gross anatomy of the stomach and to investigate the histological structure of the sacciform fore stomach region. The stomach of the kangaroo is an elongated curved bag that comprised three parts; small sacciform fore stomach, long tubiform fore stomach and small hind stomach. The sacciform fore stomach is represented by two small blind sacs, parietal and medial ones. The tubiform fore stomach is in the form of sacculated equine-colon like tube. It represent the main bulk of the stomach. It is characterized by the presence of taeniae and haustra. The wall of the parietal blind sac is composed of glandular gastric mucosa. However, in the medial sac, the lining epithelium was non glandular. Conclusion: The tubiform fore stomach represents the main part of the kangaroo stomach which allows thorough mixing of the food and enables kangaroo to digest the highly fibrous grasses. In addition, the presence of glandular mucosa at the parietal blind sac suggests a secretory function to this region which may increase the fermentation and digestibility of plant cell walls.6th scientific conference, Fac. Vet. Medicine, Mansoura University, Egypt. 01/2009;
Article: Fayed, M. H., Shoaib, M. B., Elnasharty, M. (2010): In situ identification of sugar residues in the stomach of three species of monkeys (Tupaiidae glis, Nycticebus cocang and Callithrix jacchus) by lectin histochemistry. Advances in Biological Research 4[show abstract] [hide abstract]
ABSTRACT: The parotid salivary gland (PSG) of the monkey was histochemically analyzed by lectin histochemistry aiming to investigate the features of the available sugar residues and their suggested biology. These lectins were used as probe and the horseradish peroxidase (HRP) as visualant. The intensity of lectin binding affinity of the secretory acini and the smaller excretory ducts (intercalated, striated and interlobular ducts) showed wide variations. The reaction of the serous acini was strong with the Wheat germ agglutinin (WGA), moderate with the Ulexus europeus agglutinin-1 (UEA-1), scanty with the Concanavalia ensiformes agglutinin (Con-A) and negative with Peanut agglutinin (PNA). The lining epithelial cells of the intralobular ducts (intercalated and striated ducts) showed strong reaction to WGA, Con-A and PNA. While those of the interlobular ducts were negative to all lectins used except the moderate reaction to PNA. The goblet cells of the interlobular duct gave strong reaction with WGA only. The present study suggested that, the nature and composition of stored glycoproteins in monkey PSG is heterogeneous. The predominant terminal sugar residues are supposed to be α-D-GlcNAc in secretory acini and α-D-glc and α-D-man in the ducts system.Advances in Biological Research. 01/2010; 4(1-4 (1): 01-09.):1-9.
Article: In situ identification of sugar residues in monkey’s salivary glands by lectin histochemistry. II. Parotid gland.[show abstract] [hide abstract]
ABSTRACT: Using lectin histochemistry, the stomach of three species of non-human primates was investigated to clarify their sugar residues' staining affinity and distribution patterns. N-acetylglucosamine and/or neuraminic acid (represented in positive reaction to WGA conjugate) were rich in all gastric regions with little differences between the deep and superficial parts of the same region. In the same time N-acetylgalactosamine (represented in positive reaction to PNA conjugate), α D-glucose and α D-mannose (represented in positive reaction to Con-A conjugate), were abundant in the deep parts of the gastric mucosa and were scanty or absent in the superficial regions of the gastric mucosa. In conclusion, there is a difference among the mucosubstances of surface and foveolar mucous cells, mucous neck cells, and gastric gland cells. This indicates heterogeneous composition of gastric mucus, or mucus molecules with variations in the degree of glycosylation of their oligosaccharide chains in the different cells which suggest that lectin binding affinity in the gastric mucosa correlated mostly to the degree of cellular differentiation.Global Veterinaria 4(1) 58-66. 01/2010;