Lluis Arola

Publications (204) View all

• Source

  Available from: Maria-Josepa Salvadó

  Dataset: 2007GN.Montagut

  Anna Arola, Montserrat Vaqué, Gerard Pujadas, Anna Maria Ardévol, Maria Cinta Bladé, Maria Teresa Blay, Juan Bautista Fernández-Larrea, Maria Josepa Salvadó, Lluís Arola
• Article: Flavanol metabolites distribute in visceral adipose depots after a long-term intake of grape seed proanthocyanidin extract in rats.

  Aida Serra, Cinta Bladé, Lluís Arola, Alba Macià, Maria-José Motilva
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  ABSTRACT: A considerable number of epidemiological investigations and intervention studies have supported an association between the intake of flavanol- and proanthocyanidin-containing foods and a decreased risk of metabolic diseases. Nonetheless, less is know about the capacity of tissues to accumulate flavanols and/or their metabolites. The main objective of the present study was to determine (n 20) plasma bioavailability and disposition in the liver, muscle, brown adipose tissue (BAT) and white adipose tissues (mesenteric and perirenal) in rats after a long-term consumption of three doses of grape seed phenolic extract (5, 25 and 50 mg/kg body weight) for 21 d in order to determine whether there is a dose-response relationship. Glucuronidated conjugates (total glucuronidated conjugates: C5 mg/kg 1·9; C25 mg/kg 6·4; C50 mg/kg 27·7 μmol/l plasma) followed by methyl glucuronidated conjugates (total methyl glucuronidated conjugates: C5 mg/kg 1·98; C25 mg/kg 4·48; C50 mg/kg 12·5 μmol/l plasma) were the main flavanol metabolites quantified in plasma, also detecting a dimer in its free form (C25 mg/kg 0·74; C50 mg/kg 0·79 μmol/l plasma). Each of the studied organs has a particular behaviour of accumulation and response to the assayed grape seed extract doses, with an exponential bioavailability-dose relationship in BAT, in which flavanols could play an important role in the reduction or prevention of obesity, modulating the functionality of that tissue.
  The British journal of nutrition 03/2013; · 3.45 Impact Factor
• Article: Bioavailability of procyanidin dimers and trimers and matrix food effects in in vitro and in vivo models - CORRIGENDUM.

  Aida Serra, Alba Macià, Maria-Paz Romero, Josep Valls, Cinta Bladé, Lluís Arola, Maria-José Motilva
  The British journal of nutrition 03/2013; · 3.45 Impact Factor
• Article: Assessment of compatibility between extraction methods for NMR- and LC/MS-based metabolomics.

  Antoni Beltran, Manuel Suarez, Miguel A Rodríguez, Maria Vinaixa, Sara Samino, Lluís Arola, Xavier Correig, Oscar Yanes
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  ABSTRACT: Because of the wide range of chemically and structurally diverse metabolites, efforts to survey the complete metabolome rely on the implementation of multiplatform approaches based on nuclear magnetic resonance (NMR) and mass spectrometry (MS). Sample preparation disparities between NMR and MS, however, may limit the analysis of the same samples by both platforms. Specifically, deuterated solvents used in NMR strategies can complicate LC/MS analysis as a result of potential mass shifts, whereas acidic solutions typically used in LC/MS methods to enhance ionization of metabolites can severely affect reproducibility of NMR measurements. These intrinsically different sample preparation requirements result in the application of different procedures for metabolite extraction, which involve additional sample and unwanted variability. To address this issue, we investigated 12 extraction protocols in liver tissue involving different aqueous/organic solvents and temperatures that may satisfy the requirements for both NMR and LC/MS simultaneously. We found that deuterium exchange did not affect LC/MS results, enabling the measurement of metabolites by NMR and, subsequently, the direct analysis of the same samples by using LC/MS with no need for solvent exchange. Moreover, our results show that the choice of solvents rather than the temperature determined the extraction efficiencies of metabolites, a combination of methanol/chloroform/water and methanol/water being the extraction methods that best complement NMR and LC/MS analysis for metabolomic studies.
  Analytical Chemistry 06/2012; 84(14):5838-44. · 5.86 Impact Factor
• Article: Detection of bioavailable peroxisome proliferator-activated receptor gamma modulators by a cell-based luciferase reporter system.

  Josep M del Bas, Sirle Laos, Antoni Caimari, Anna Crescenti, Lluís Arola
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  ABSTRACT: In vitro cell-based reporter assays are a useful tool for the discovery and characterization of nuclear receptor modulators. However, the properties of a given molecule can differ when tested in vitro and in vivo as a result of the molecule's bioavailability. In this work, we describe a methodology that allows the detection of the PPARγ (peroxisome proliferator-activated receptor gamma) agonist bezafibrate in rat serum by an in vitro cell-based reporter assay. This methodology could be adapted to the detection and characterization of bioavailable PPARγ or other nuclear receptor modulators in serum, extending the possibilities of the classical in vitro assays.
  Analytical Biochemistry 05/2012; 427(2):187-9. · 3.00 Impact Factor