Leucio Alves |
|
|
|
Universidade Federal Rural de Pernambuco
·
Department of Veterinary Medicine
|
Publications (3) View all
-
Article: Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil).
Rafael Ramos, Carlos Ramos, Flábio Araújo, Renato Oliveira, Ingrid Souza, Danillo Pimentel, Mariana Galindo, Marilia Santana, Eduardo Rosas, Maria Faustino, Leucio Alves[show abstract] [hide abstract]
ABSTRACT: To identify DNA of the main tick-borne pathogens in dogs from Recife (Brazil), polymerase chain reactions were carried out on blood samples of dogs treated at the Veterinary Hospital of the Universidade Federal Rural de Pernambuco from March 2007 to June 2008. The detection of DNA was performed using specific primers. Amplicons were analyzed through electrophoresis and sequencing. A phylogenetic tree was constructed using the UPGMA method, revealing that the sequences were closely related to those of strains from other geographic regions. Among the 205 blood samples analyzed, 48.78% was positive for Anaplasma platys; 38.04% was positive for Ehrlichia canis; 7.31% was positive for Babesia canis vogeli; and 0.49% was positive for Hepatozoon canis and Mycoplasma haemocanis. Coinfection of two or three pathogens was found in 23.9% (49/205) of the dogs. The subspecies B. canis vogeli was identified. Infection by H. canis and M. haemocanis is reported for the first time in dogs in the state of Pernambuco (Brazil). The data indicate that the main tick-borne pathogens in dogs in this region are E. canis and/or A. platys, followed by B. canis vogeli.Parasitology Research 10/2010; 107(5):1115-20. · 2.15 Impact Factor -
Article: [Detection of antibodies against Anaplasma sp. in small ruminants from the semi-arid region against Pernambuco State, Brazil].
Rafael A N Ramos, Carlos A N Ramos, Flábio R Araújo, Elaine S P Melo, Antônio A S M Tembue, Maria A G Faustino, Leucio C Alves, Grácia M S Rosinha, Carina Elisei, Cleber O Soares[show abstract] [hide abstract]
ABSTRACT: This paper reports the detection of antibodies against Anaplasma sp. in goats and sheep from the semi-arid region from Pernambuco State, Brazil, using an enzyme-linked immunosorbent assay with recombinant MSP5 of Anaplasma marginale. Sera from 243 goats and 68 sheep from Ibimirim municipality were analyzed and frequencies of antibodies of 11.93% (29/243) and 16.17% (11/68) were found for goats and sheep, respectively. The epidemiological relevance of the findings was discussed.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria 17(2):115-7. · 0.46 Impact Factor -
Article: [Occurrence of Cryptosporidium spp. infection in antillean manatee (Trichechus manatus)].
João Carlos Gomes Borges, Leucio Câmara Alves, Jociery Einhardt Vergara-Parente, Maria Aparecida da Glória Faustino, Erilane de Castro Lima Machado[show abstract] [hide abstract]
ABSTRACT: Cryptosporidiosis is a zoonosis which can affect man and a wide range of domestic and wild animals, mainly immunodeficient individuals. The objective of this paper was reported the occurrence of a Cryptosporidium infection in Antillean manatee. After an unusual behavior of an Antillean manatee kept in captivity at the Centro Mamíferos Aquáticos, ICMBio--FMA, clinical examination and posterior fecal sampling was performed. Fecal samples were examined by the Kinyoun technique, Direct Immunofluorescence Test and also examined by 4',6'-Diamidino-2-Phenylindole (DAPI) staining. At the clinical examination, the animal showed signs of abdominal pain. The results obtained by light and fluorescence microscopy analysis showed the presence of Cryptosporidium spp. oocyst in feces of this manatee.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria 18(1):60-1. · 0.46 Impact Factor