Kumar Vel

Questions and Answers (12) View all

• Question asked in Plant Tissue Culture
  3 How to differentiate embryogenic and non embryogenic calli based on the cell structure?
  Experts can differentiate embryogenic calli from non embryogenic calli based on the morphological appearance. But for beginners it's a tough task to c... [more]
  Experts can differentiate embryogenic calli from non embryogenic calli based on the morphological appearance. But for beginners it's a tough task to carry out. Is it possible to differentiate embryogenic and non embryogenic calli based on the cell structure and the organelles which are present in it? If so kindly suggest some ideas what cells of embryogenic calli looks like?
  By Kumar Vel · National Research Centre for Banana
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• Answer added in Protein Expression
  10 How to identify the proteins of an incompleted genome sequence of an organism like Banana?
  By Kumar Vel · National Research Centre for Banana
  Kumar Vel · National Research Centre for Banana

  @Mispah Mustaffa thanks for your suggestion....as i mentioned earlierthe database for Musa AA genome is available but for BB genome its still lackin... [more]

  @Mispah Mustaffa thanks for your suggestion....as i mentioned earlierthe database for Musa AA genome is available but for BB genome its still lacking...So the one who is working on triploid genome cultivar(AAB,ABB) have to go for alternate genome database like EST translated nucleotide db...where we can find out our differentially expressed proteins for comparitive proteomic studies.In comparitive proteomics mainly we are focusing on control and experimental samples(either undergo biotic/abiotic stress).Thats y we are focusing on stress related proteins which may play various role in defense mechanism....
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• Answer added in Protein Expression
  10 How to identify the proteins of an incompleted genome sequence of an organism like Banana?
  By Kumar Vel · National Research Centre for Banana
  Kumar Vel · National Research Centre for Banana

  thanks for your reply...i posted this question a year back...now the seq for AA Genome is completed.So now its some how easy to identify the proteins ... [more]

  thanks for your reply...i posted this question a year back...now the seq for AA Genome is completed.So now its some how easy to identify the proteins through that available BANANA GENOME DATABASE from CIRAD....
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• Question asked in IEF
  1 Can I set 5500V as a maximum volt for 13cm strip instead of 8000V?
  While doing Ief in 13cm strip, up to 5500 Volt the condition is working good. Once it goes beyond 6000v,the bubbles start coming in the negative termi... [more]
  While doing Ief in 13cm strip, up to 5500 Volt the condition is working good. Once it goes beyond 6000v,the bubbles start coming in the negative terminal of the electrode point.Next time i tried by adding 10% glycerol in the re-swelling buffer in order to remove the electro osmotic flow of water current. But the problems didn't rectify, it happens again when it reaches 6000v.
  By Kumar Vel · National Research Centre for Banana
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• Answer added in Protein Analysis
  5 What is the exact reason for not attaining high voltage(8000v) while running IEF for callus protein sample? Can anyone suggest an idea?
  By Kumar Vel · National Research Centre for Banana
  Kumar Vel · National Research Centre for Banana

  Thanks Bita... for your suggestions.For desalting we are giving 80%acetone wash twice and then we are air drying the pellet.Also i will try with that... [more]

  Thanks Bita... for your suggestions.For desalting we are giving 80%acetone wash twice and then we are air drying the pellet.Also i will try with that miniamicon.
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