Kishore babu Turlapati

Questions and Answers (13) View all

• Answer added in Biotechnology
  8 Tricine SDS-PAGE
  By Abheepsa Mishra · IIT Kharagpur
  Kishore Turlapati · Agharkar Research Institute

  Wash your gel plates properly and Prepare APS freshely, Check contamination your TEMED and Acylamide and Bis acrylamide. 

  Wash your gel plates properly and Prepare APS freshely, Check contamination your TEMED and Acylamide and Bis acrylamide.
  Following
• Answer added in Enzymology
  17 Is there any method to harvest intracellular enzymes without the lysis of cells?
  By Anuma Singh · Indian Institute of Technology Guwahati
  Kishore Turlapati · Agharkar Research Institute

  There is no possibility for isolation of intracellular proteins with out lysis of cells. 

  There is no possibility for isolation of intracellular proteins with out lysis of cells.
  Following
• Answer added in Chromatography
  2 How to prepare beds for working?
  By Nourhan Mohammad · Alexandria University
  Kishore Turlapati · Agharkar Research Institute

  What is the type of expression is it extracellular, ot Intracellular? What was the charge of your molecule? what was the size of your miolecule?,ple... [more]

  What is the type of expression is it extracellular, ot Intracellular? What was the charge of your molecule? what was the size of your miolecule?,please confirm these Regards Kishorebabu T
  Following
• Answer added in Chromatography
  6 Column chromatography separation of plant compounds?
  By Pradeep Shrestha · Tribhuvan University
  Kishore Turlapati · Agharkar Research Institute

  Can you eloberate what was your desired compound in your extract Kishorebabu T 

  Can you eloberate what was your desired compound in your extract Kishorebabu T
  Following
• Answer added in Protein Purification
  20 Protein purification
  By Sayem Rahman · Griffith University
  Kishore Turlapati · Agharkar Research Institute

  Dear Sayem, After solubilization, perform refolding in step dilution method, this will help you for decresing the urea concentration. Maintain less c... [more]

  Dear Sayem, After solubilization, perform refolding in step dilution method, this will help you for decresing the urea concentration. Maintain less conductivity during refolding.Then Perform concentration and dialysis. Regards
  Following