Kate Reddington

Publications (7) View all

• Source

  Available from: Justin O'Grady

  Article: Advances in multiparametric molecular diagnostics technologies for respiratory tract infections.

  Kate Reddington, Nina Tuite, Thomas Barry, Justin O'Grady, Alimuddin Zumla
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  ABSTRACT: PURPOSE OF REVIEW: Respiratory tract infections (RTIs) are caused by a variety of bacterial, viral, fungal, and other pathogens and cause millions of deaths each year. Current standard microbiological culture-based tests are laborious and time consuming. Thus, patients are initially treated empirically, leading to inappropriate use of antibiotics. The purpose of this article is to provide clinicians and scientists with a review of recently available commercial multiparametric molecular diagnostics tests for the detection of RTIs so that they can be considered for use instead of, or in combination with, traditional culture techniques. RECENT FINDINGS: Several technologies have become commercially available for the multiparametric molecular detection of RTIs in the past decade including tests based on PCR-array, PCR-mass spectrometry, and multiplex qPCR technologies. The majority of these tests are for the detection of viruses, but more recently companies have begun to focus on detection of viruses, bacteria, and associated drug resistances in a single product to maximize the information provided to the clinician by a single test. SUMMARY: We describe the recent advances in commercial multiparametric molecular diagnostics technologies for the diagnosis of RTIs. Combining the specific and sensitive molecular detection of bacteria, viruses, fungi, and drug resistances is key if molecular methods are to replace traditional culture. The reliability of the molecular drug-resistance markers chosen, the need for the quantitative detection of some organisms, and throughput are also important considerations for new technology developers.
  Current opinion in pulmonary medicine 02/2013; · 3.08 Impact Factor
• Source

  Available from: Justin O'Grady

  Article: Evaluation of a novel Listeria enrichment broth combined with a real-time PCR diagnostics assay for the specific detection of Listeria monocytogenes in RTE pork products

  Diego G Omez, Sheila Mcguinness, Kate Reddington, Justin O'Grady, Javier Yanguela, Thomas Barry
  International Journal of Food Science & Technology 01/2013; · 1.26 Impact Factor
• Source

  Available from: Justin O'Grady

  Chapter: Molecular Diagnosis of Active Pulmonary Tuberculosis

  Anna Bateson, Kate Reddington, Justin O'Grady
  01/2013;
• Source

  Available from: Justin O'Grady

  Article: SeekTB, a two-stage multiplex real-time-PCR-based method for differentiation of the Mycobacterium tuberculosis complex.

  Kate Reddington, Alimuddin Zumla, Matthew Bates, Dick van Soolingen, Stefan Niemann, Thomas Barry, Justin O'Grady
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  ABSTRACT: Tuberculosis (TB) in humans is caused by members of the Mycobacterium tuberculosis complex (MTC). The accurate identification of the MTC member causing human infection is important because the treatment of TB caused by some MTC members requires an alteration of the standard drug regimen, it can inform whether transmission is human to human or zoonotic, and it enables accurate epidemiology studies that help improve TB control. In this study, an internally controlled two-stage multiplex real-time PCR-based method, SeekTB, was developed for the accurate identification of all members of the MTC. The method was tested against a panel of well-characterized bacterial strains (n = 180) and determined to be 100% specific for members of the MTC. Additionally, 125 Mycobacteria Growth Indicator Tube (MGIT)-positive cultures were blindly tested by using SeekTB, and the results were compared to those of the GenoType MTBC and TBc ID tests. The SeekTB and GenoType MTBC results were 100% concordant, identifying 84 of these isolates as M. tuberculosis isolates and 41 as non-MTC isolates. Nine discordant results between the molecular methods and the TBc ID culture confirmation test were observed; however, nucleotide sequencing confirmed the results obtained with GenoType MTBC and SeekTB. SeekTB is the first-described internally controlled multiplex real-time PCR diagnostic method for the accurate identification of all eight members of the MTC. This method, designed for use on cultured patient samples, is specific, sensitive, and rapid, with a turnaround time to results of approximately 1.5 to 3.5 h, depending on which, if any, member of the MTC is present.
  Journal of clinical microbiology 05/2012; 50(7):2203-6. · 4.16 Impact Factor
• Article: Culture confirmation of Listeria monocytogenes using tmRNA as a diagnostics target.

  Eoin Clancy, Barry Glynn, Kate Reddington, Terry Smith, Thomas Barry
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  ABSTRACT: 16s ribosomal RNA (rRNA) is routinely used to identify bacteria in direct detection culture confirmation assays. In some instances rRNA cannot be used as a target to distinguish between phylogenetically closely related bacteria. Here we evaluate an alternative target, transfer messenger RNA (tmRNA), for the culture confirmation of Listeria monocytogenes.
  Journal of microbiological methods 03/2012; 88(3):427-9. · 2.43 Impact Factor