Karen Reece
PhD, Physiology, University of...
Promega · Nucleic Acid Technologies

Topics (16) View all

Education

  • Jul 2000–
    Nov 2007
    University of Wisconsin
    Physiology · PhD
    USA · Madison, WI
  • Jul 2000–
    Aug 2002
    University of Wisconsin - Madison
    Physiology · MS
    USA · Madison
  • Sep 1996–
    May 2000
    University of Wisconsin - Madison
    Biochemistry · BS
    USA · Madison

Other

Questions and Answers (27) View all

  • Answer added in DNA Extraction
    3 Quality DNA from swabs samples
    By Jim Mcmichael · Bucknell University
    Karen Reece · Promega
    It looks like your problem is less with protein contamination and more with some kind of carryover (e.g. guanidine) from your purification causing hig... [more]

Publications (2) View all

  • Article: Intramolecular interactions in the N-domain of cardiac troponin C are important determinants of calcium sensitivity of force development.
    Karen L Reece, Richard L Moss
    [show abstract] [hide abstract]
    ABSTRACT: Myocardial contraction is initiated when Ca2+ binds to site II of cardiac troponin C. This 12-residue EF-hand loop (NH2-DEDGSGTVDFDE-COOH) contains six residues (bold) that coordinate Ca2+ binding and six residues that do not appear to influence Ca2+ binding directly. We have introduced six single-cysteine substitutions (italics) within site II of cTnC to investigate whether these residues are essential for Ca2+ binding affinity in isolation and Ca2+ sensitivity of force development in single muscle fibers. Ca2+ binding properties of mutant proteins were examined in solution and after substitution into rat skinned soleus fibers. Except for the serine mutation, cysteine substitution had no effect on Ca2+ binding on cTnC in solution. However, as part of the myofilament, the threonine mutation reduced Ca2+ sensitivity while the phenylalanine mutation increased Ca2+ sensitivity. Analysis of the available crystal and NMR structures reveals specific structural mechanisms for these effects.
    Biochemistry 06/2008; 47(18):5139-46. · 3.42 Impact Factor
  • Article: Removal of contaminating calcium from buffer solutions used in calcium binding assays.
    Karen L Reece, Richard L Moss
    Analytical Biochemistry 07/2007; 365(2):274-6. · 3.00 Impact Factor

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